MG132 allows extensive RT of HIV-1 cDNA despite exogenous rhTRIM5α.

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MG132 allows extensive RT of HIV-1 cDNA despite exogenous rhTRIM5α. MG132 allows extensive RT of HIV-1 cDNA despite exogenous rhTRIM5α. (A) Location of DNA digest sites and probes for Southern blot analysis of HIV-1 5′ (probe 1) and 3′ (probe 2) cDNA ends. (B–D) Southern blot analysis of uninfected (−) or R7-GFPHIV-1 infected HeLa or rhTRIM5α.HA stable HeLa (Rh HA) cells without (V) or with MG132 (MG). The same blot probed for 5′ (B) or 3′ (C) HIV-1 cDNA or GAPDH for DNA loading (D) is shown. Asterisks mark residual probe 2. Although late RT products with complete ends escape rhTRIM5α restriction via MG132, 1-LTR and 2-LTR circles remain impaired. Xiaolu Wu et al. PNAS 2006;103:19:7465-7470 ©2006 by National Academy of Sciences