Protein synthesis: Twenty three amino acids and counting

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Protein synthesis: Twenty three amino acids and counting Michael Ibba, Constantinos Stathopoulos, Dieter Söll Current Biology Volume 11, Issue 14, Pages R563-R565 (July 2001) DOI: 10.1016/S0960-9822(01)00344-X

Fig. 1 Schematic representation of the selection of an orthogonal tRNA–aminoacyl-tRNA pair for the in vivo site-specific incorporation of O-methyl-L-tyrosine in E. coli (see text for details). Separate selections were first applied to obtain a pool of mutant tyrosyl-tRNA synthetase variants and an orthogonal derivative of tRNATyrCUA (a variant of tRNATyr where the anticodon has been changed to generate an amber suppressor tRNA). These two components were then brought together and used to select particular tyrosyl-tRNA synthetase mutants that preferentially recognize O-methyl-l-tyrosine rather than tyrosine. TyrRS, tyrosyl-tRNA synthetase; AARS, aminoacyl-tRNA synthetase; AA, amino acid. Current Biology 2001 11, R563-R565DOI: (10.1016/S0960-9822(01)00344-X)