PH-Dependent Conformation, Dynamics, and Aromatic Interaction of the Gating Tryptophan Residue of the Influenza M2 Proton Channel from Solid-State NMR 

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Presentation transcript:

pH-Dependent Conformation, Dynamics, and Aromatic Interaction of the Gating Tryptophan Residue of the Influenza M2 Proton Channel from Solid-State NMR  Jonathan K. Williams, Yuan Zhang, Klaus Schmidt-Rohr, Mei Hong  Biophysical Journal  Volume 104, Issue 8, Pages 1698-1708 (April 2013) DOI: 10.1016/j.bpj.2013.02.054 Copyright © 2013 Biophysical Society Terms and Conditions

Figure 1 Several Trp41 rotamers that have been proposed in the literature using various experimental techniques (Table 1). Population percentages for α-helices from the Penultimate Rotamer Library are shown (42). (Left column) Side view of two of the four helices; both His37 and Trp41 side chains are depicted. Note the shortest distances between these two residues are between two adjacent helices rather than from the same helix. (Right column) Top view of the four helices and the Trp41 sidechains. (a) t90 rotamer (χ1 = 180°, χ2 = +90°). (b) t-105 rotamer (χ1 = 180°, χ2 = −105°). (c) m95 rotamer (χ1 = −60°, χ2 = +95°). Biophysical Journal 2013 104, 1698-1708DOI: (10.1016/j.bpj.2013.02.054) Copyright © 2013 Biophysical Society Terms and Conditions

Figure 2 Trp41 chemical shifts at pH 4.5 and 8.5 in LW-M2TM bound to the VM+ membrane. (a) Aromatic region of the 1D 13C cross-polarization spectra at pH 4.5 and 8.5. (b) Aliphatic region of the 1D 13C DQF spectra at the two pH conditions. The lipid peaks were suppressed by the double-quantum filter. Note the chemical shift changes of Trp41 and Leu40 Cα and Cβ peaks, indicating pH-induced small conformation changes of the helix backbone. (c) 2D 13C-13C DQF spectrum of LW-M2TM at pH 8.5. (d) 2D 15N-13C correlation spectrum of the peptide at high pH. (e) Summary of Trp41 13C and 15N chemical shifts at high pH (red) and low pH (green). (Black) High-pH chemical shifts of M2(22–62) in DOPC/DOPE bilayers (38) for sites that have >0.5 ppm chemical shift difference from the M2TM values. (Bold) Chemical shifts that differ by >0.5 ppm between high and low pH. All 13C chemical shifts are reported on the tetramethylsilane scale. Biophysical Journal 2013 104, 1698-1708DOI: (10.1016/j.bpj.2013.02.054) Copyright © 2013 Biophysical Society Terms and Conditions

Figure 3 13C-19F REDOR distance measurements of 13C-labeled His37 and 5-19F-labeled Trp41 in VM bound M2TM. (a) Approximate spatial arrangement of His37 of one helix and Trp41 of the neighboring helix. (b) Representative 13C-detected 19F-dephased REDOR control (S0, black) and dephased (S, red) spectra of the peptide at pH 8.5. The spectra were measured at 243 K under 3.3 kHz MAS. (c) Representative REDOR spectra of the peptide at pH 4.5. The spectra were measured under 3.0, 3.3, and 6.7 kHz MAS in order to detect all His37 aromatic 13C signals without overlap from the carbonyl sidebands (asterisks). (d) 13C-19F REDOR S/S0 intensity as a function of mixing time for the high-pH (red) and low-pH (green) peptides. De novo two-spin simulations are shown. Best-fit distances are highlighted for the high-pH data (red) and the low-pH data (green). Biophysical Journal 2013 104, 1698-1708DOI: (10.1016/j.bpj.2013.02.054) Copyright © 2013 Biophysical Society Terms and Conditions

Figure 4 Total RMSD between the measured and modeled Cα-F, Cγ-F, Cε1-F, Cδ2-F, and 19F-19F distances as a function of Trp41 (χ1, χ2) torsion angles. Different backbone structure models for M2TM at high (a and b) and low (c and d) pH are used for structural modeling. Only the shortest of the four possible distances in the tetramer for each two-spin combination is used to compare with the experimental data. (a) PDB:3LBW backbone structure. (b) PDB:2KQT backbone structure. (c) PDB:3C9J backbone structure. (d) PDB:2KAD backbone structure. Between the two high-pH backbone structures, consensus (χ1, χ2) results were found at (−175°, 120°). For the low-pH state, the best-fit Trp41 rotamer depends on the backbone structure. For the PDB:2KAD structure (d), the best-fit solution is (χ1, χ2) = (−155°, 135°), whereas the PDB:3C9J structure (c) shows two rotamer minima without steric conflict. (Crosses) Rotamers with steric clashes, which are not considered further (see Fig. S2). Biophysical Journal 2013 104, 1698-1708DOI: (10.1016/j.bpj.2013.02.054) Copyright © 2013 Biophysical Society Terms and Conditions

Figure 5 His37-Trp41 contacts and Trp41 side-chain conformation at high and low pH. (a) Best fits of the 13C-19F REDOR and 19F-19F CODEX data at high pH, using PDB:2KQT for the backbone structure and a Trp41 rotamer of χ1 = −175° and χ2 = +120°. (Red and black curves) Model-dependent five-spin and two-spin best-fit simulations. (b) Best fits of the 13C-19F REDOR and 19F-19F CODEX data at low pH, using PDB:2KAD as the backbone structure and a Trp41 rotamer of χ1 = −155° and χ2 = +135°. (c) His-Trp region of the high-pH structure (PDB:2KQT), with Trp41 (χ1, χ2) angles of (−175°, 120°). (d) His-Trp region of the low-pH structure (PDB:2KAD), where the Trp41 rotamer is (−155°, 135°). (Dashed lines) Relevant His-Trp 13C-19F distances. At low pH, the imidazole and indole rings approach each other more closely, due to the combined effect of the helix backbone orientation change and Trp41 (χ1, χ2) changes. Biophysical Journal 2013 104, 1698-1708DOI: (10.1016/j.bpj.2013.02.054) Copyright © 2013 Biophysical Society Terms and Conditions

Figure 6 Trp41 side-chain dynamics at high pH (red) and low pH (green) in VM+ membranes at 303 K. (a and b) Representative 13C-1H DIPSHIFT curves for (a) Cδ1-H and (b) Cζ3-H couplings. (c) Cε2-Nε1 REDOR dephasing curve. (d) Summary of all dipolar order parameters of the indole ring at high and low pH. Cδ1-H and Cζ3-H bonds show different order parameters between high (red) and low (green) pH. Most order parameters have an experimental uncertainty of ±0.05. (e) Calculated order parameters as a function of the standard deviations (σ) of the Gaussian fluctuations around the Cα-Cβ and Cβ-Cγ bonds. Only values that agree with the measured low-pH SCH are shown. Small-amplitude Gaussian fluctuations with σ1 ≈ 30° and σ2 ≈ 15° (shaded area) agree with all measured order parameters. (f) Calculated order parameters that agree with the high-pH SCH values. Only torsional motion around the χ1 axis is compatible with the experimental data, with σ1 ≈ 25° (indicated by a star). Biophysical Journal 2013 104, 1698-1708DOI: (10.1016/j.bpj.2013.02.054) Copyright © 2013 Biophysical Society Terms and Conditions

Figure 7 Trp41 conformational dynamics and aromatic interaction with His37. (a) Low-pH scenario. The measured equilibrium (χ1, χ2) rotamer of (−155°, 135°) is shown as position 2, together with two limiting rotamers based on the measured order parameters. The (−125°, 150°) rotamer (position 3) is closer to His37 whereas the (175°,120°) rotamer (position 1) is further away from His37. These angles were chosen to be one standard deviation (30° for σ1 and 15° for σ2) from the average angle. (b) High-pH scenario. The equilibrium rotamer is (−175°, 120°) (position 2), and the limiting rotamers are (−150°,120°) (position 1) and (160°,120°) (position 3), based on a σ1 of 25° and no χ2 fluctuation. Biophysical Journal 2013 104, 1698-1708DOI: (10.1016/j.bpj.2013.02.054) Copyright © 2013 Biophysical Society Terms and Conditions