Polymerase Chain Reaction

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Presentation transcript:

Polymerase Chain Reaction

DNA

PCR DNA replication can also be performed in vitro (artificially, outside a cell). The polymerase chain reaction (PCR), a common laboratory technique, cyclically applies such artificial synthesis to amplify a specific target DNA fragment from a pool of DNA. Kary Mullis 1993

PCR

PCR

Temperate procedure

Thermocyclers Conventional PCR

Detection of genetic mutations Virus detection and Genotyping of the hepatitis virus Help in the diagnosis of certain diseases . Used in cloning and Sequencer of DNA Detection the length of DNA Technology of c DNA (complementary DNA ( Determine the desired gene from a mixture of genes Human genome project Diagnosed of cancer Organ transplantation

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