Next-Generation Stool DNA Testing: Expanding the Scope David A. Ahlquist  Gastroenterology  Volume 136, Issue 7, Pages 2068-2073 (June 2009) DOI: 10.1053/j.gastro.2009.04.025 Copyright © 2009 AGA Institute Terms and Conditions

Figure 1 Detection of tissue-confirmed mutations in matched stools from patients with colorectal cancer using the digital melt curve assay. (A, B) Shifted melt and difference curves showing a deletion in the APC gene detected at 0.6% mutation level. (C) Sequencing confirmed stool mutation. (D, E) Shifted melt and difference curves showing a substitution in a TP53 exon detected at 0.8% mutation level. (F) Sequencing confirmed stool mutation. From Zou et al8 (Reprinted with permission). Gastroenterology 2009 136, 2068-2073DOI: (10.1053/j.gastro.2009.04.025) Copyright © 2009 AGA Institute Terms and Conditions

Figure 2 Blinded comparison of stool tests for detection of advanced adenomas in the screening setting. Results from digital melt curve (DMC) DNA test compared with those from Hemoccult, HemoccultSENSA, and EXACT PreGenPlus DNA test. (A) Sensitivities for advanced adenomas with tissue-proven KRAS mutations (n = 27). The DMC assay detected significantly more adenomas than the other tests (P < .05 vs each other test). (B) Specificities based on stools from age- and gender-matched controls; differences were not statistically significant. From Zou et al8 (with permission). Consider changing figure labels to “PreGen Plus” instead of “EXACT.” Gastroenterology 2009 136, 2068-2073DOI: (10.1053/j.gastro.2009.04.025) Copyright © 2009 AGA Institute Terms and Conditions