The Effects of Cyclooxygenase Isozyme Inhibition onIncisional Wound Healing in Mouse Skin Karin Müller-Decker, Wolfgang Hirschner, Friedrich Marks, Gerhard.

Slides:

Advertisements

Similar presentations

EGFR Enhances Early Healing After Cutaneous Incisional Wounding

Advertisements

Induction of Bone Morphogenetic Protein-6 in Skin Wounds

The Tumor Necrosis Factor Superfamily Molecule LIGHT Promotes Keratinocyte Activity and Skin Fibrosis Rana Herro, Ricardo Da S. Antunes, Amelia R. Aguilera,

Interleukin-22 Promotes Wound Repair in Diabetes by Improving Keratinocyte Pro- Healing Functions Simona Avitabile, Teresa Odorisio, Stefania Madonna,

Integrin α2β1 Is Required for Regulation of Murine Wound Angiogenesis but Is Dispensable for Reepithelialization Manon C. Zweers, Jeffrey M. Davidson,

Activated Kras Alters Epidermal Homeostasis of Mouse Skin, Resulting in Redundant Skin and Defective Hair Cycling Anandaroop Mukhopadhyay, Suguna R.

C-Myc activation in transgenic mouse epidermis results in mobilization of stem cells and differentiation of their progeny Isabel Arnold, Fiona M Watt

Counterregulation of Interleukin-18 mRNA and Protein Expression During Cutaneous Wound Repair in Mice Heiko Kämpfer, Heiko Mühl, Josef Pfeilschifter,

Impaired Wound Repair in Adult Endoglin Heterozygous Mice Associated with Lower NO Bioavailability Eduardo Pérez-Gómez, Mirjana Jerkic, Marta Prieto,

Ectodysplasin A Pathway Contributes to Human and Murine Skin Repair

Accelerated Wound Repair in ADAM-9 Knockout Animals

Tumor Necrosis Factor-α-Activated Human Adipose Tissue–Derived Mesenchymal Stem Cells Accelerate Cutaneous Wound Healing through Paracrine Mechanisms

Severely Impaired Wound Healing in the Collagenase-Resistant Mouse

Vitali Alexeev, Kyonggeun Yoon Journal of Investigative Dermatology

Manuela Schmidt, Danny Gutknecht, Jan C

Exploring the “Hair Growth–Wound Healing Connection”: Anagen Phase Promotes Wound Re-Epithelialization David M. Ansell, Jennifer E. Kloepper, Helen A.

Estrogen Receptor α, but not Estrogen Receptor β, is Involved in the Regulation of the Hair Follicle Cycling as well as the Thickness of Epidermis in.

Epidermal Label-Retaining Cells: Background and Recent Applications

Raija Tammi Journal of Investigative Dermatology

Cyclooxygenase-1-Coupled Prostaglandin Biosynthesis Constitutes an Essential Prerequisite for Skin Repair Heiko Kämpfer, Lutz Bräutigam, Gerd Geisslinger,

Vanilloid Receptor-1 (VR1) is Widely Expressed on Various Epithelial and Mesenchymal Cell Types of Human Skin Enikő Bodó, Ilona Kovács, Andrea Telek,

The Spatial and Temporal Expression Patterns of Integrin α9β1 and One of Its Ligands, the EIIIA Segment of Fibronectin, in Cutaneous Wound Healing Purva.

P-Selectin Glycoprotein Ligand-1 Contributes to Wound Healing Predominantly as a P- Selectin Ligand and Partly as an E-Selectin Ligand Hajime Tomita,

Label-Retaining Cells (Presumptive Stem Cells) of Mice Vibrissae Do Not Express Gap Junction Protein Connexin 43 Maja Matic, Marcia Simon Journal of.

Toll-Like Receptor 4 Has an Essential Role in Early Skin Wound Healing

Efficient Healing Takes Some Nerve: Electrical Stimulation Enhances Innervation in Cutaneous Human Wounds Elaine Emmerson Journal of Investigative Dermatology

Active Hair Growth (Anagen) is Associated with Angiogenesis

Integrin β6-Deficient Mice Show Enhanced Keratinocyte Proliferation and Retarded Hair Follicle Regression after Depilation Yanshuang Xie, Kevin J. McElwee,

Localization of Plasminogen Activator Inhibitor Type 2 (PAI-2) in Hair and Nail: Implications for Terminal Differentiation Robert M. Lavker, Barbara.

Characterization of the Progressive Skin Disease and Inflammatory Cell Infiltrate in Mice with Inhibited NF-κB Signaling Max van Hogerlinden, Barbro.

Stromelysin-2 is Upregulated During Normal Wound Repair and is Induced by Cytokines Oona Rechardt, Maarit Vaalamo, Johanna Höök-Nikanne, Ulpu Saarialho-Kere

Toshiyuki Yamamoto, Kiyoshi Nishioka

MAPKAPK-2 Signaling Is Critical for Cutaneous Wound Healing

The Loss of MCP-1 Attenuates Cutaneous Ischemia–Reperfusion Injury in a Mouse Model of Pressure Ulcer Yuki Saito, Minoru Hasegawa, Manabu Fujimoto, Takashi.

Decrease in Epidermal CD44 Expression as a Potential Mechanism for Abnormal Hyaluronate Accumulation in Superficial Dermis in Lichen Sclerosus et Atrophicus

The Neurofibromatosis Type 1 (Nf1) Tumor Suppressor is a Modifier of Carcinogen- Induced Pigmentation and Papilloma Formation in C57BL/6 Mice Radhika.

Hair Cycling and Wound Healing: To Pluck or Not to Pluck?

Epithelial Stem Cells: A Folliculocentric View

Different Consequences of β1 Integrin Deletion in Neonatal and Adult Mouse Epidermis Reveal a Context-Dependent Role of Integrins in Regulating Proliferation,

Involvement of the CXCL12/CXCR4 Pathway in the Recovery of Skin Following Burns Shani Avniel, Zaretski Arik, Alex Maly, Assa Sagie, Hanna Ben Basst, Merav.

The Function of Nitric Oxide in Wound Repair: Inhibition of Inducible Nitric Oxide- Synthase Severely Impairs Wound Reepithelialization Birgit Stallmeyer,

Regulation of MMP-2 Gene Transcription in Dermal Wounds

Epithelial Cells in the Hair Follicle Bulge do not Contribute to Epidermal Regeneration after Glucocorticoid-Induced Cutaneous Atrophy Dmitry V. Chebotaev,

Bing-Mei Zhu, Yuko Ishida, Gertraud W

Wound Healing Is Defective in Mice Lacking Tetraspanin CD151

Epidermal Stem Cells do not Communicate Through Gap Junctions

Amrit Mann, Kerstin Niekisch, Peter Schirmacher, Manfred Blessing

The Suppressor of Cytokine Signaling (SOCS)-3 Determines Keratinocyte Proliferative and Migratory Potential during Skin Repair Andreas Linke, Itamar.

UVB Irradiation Stimulates Deposition of New Elastic Fibers by Modified Epithelial Cells Surrounding the Hair Follicles and Sebaceous Glands in Mice

Molecular Imaging-Assisted Optimization of Hsp70 Expression during Laser-Induced Thermal Preconditioning for Wound Repair Enhancement Gerald J. Wilmink,

Successful Treatment of Alopecia Areata-Like Hair Loss with the Contact Sensitizer Squaric Acid Dibutylester (SADBE) in C3H/HeJ Mice P.I.A. Freyschmidt-Paul,

Wound Healing in the α2β1 Integrin-Deficient Mouse: Altered Keratinocyte Biology and Dysregulated Matrix Metalloproteinase Expression David G. Grenache,

Murine Epidermal Label-Retaining Cells Isolated by Flow Cytometry do not Express the Stem Cell Markers CD34, Sca-1, or Flk-1 Michael R. Albert, Ruth-Ann.

The Nf1 Tumor Suppressor Regulates Mouse Skin Wound Healing, Fibroblast Proliferation, and Collagen Deposited by Fibroblasts Radhika P. Atit, Maria J.

John E. Olerud, Marcia L. Usui, Deniz Seckin, Diane S. Chiu, Claire L

An Extended Epidermal Response Heals Cutaneous Wounds in the Absence of a Hair Follicle Stem Cell Contribution Abigail K. Langton, Sarah E. Herrick,

Therapy for Alopecia Areata in Mice by Stimulating the Hair Cycle with Parathyroid Hormone Agonists Linked to a Collagen-Binding Domain Ranjitha Katikaneni,

Normal Wound Healing in Mice Deficient for Fibulin-5, an Elastin Binding Protein Essential for Dermal Elastic Fiber Assembly Qian Zheng, Jiwon Choi,

Betacellulin Regulates Hair Follicle Development and Hair Cycle Induction and Enhances Angiogenesis in Wounded Skin Marlon R. Schneider, Maria Antsiferova,

Themis R. Kyriakides, Jessica W.Y. Tam, Paul Bornstein

Yunyuan Li, Edward E. Tredget, Abdi Ghaffari, Xiaoyue Lin, Ruhangiz T

Ectopic Expression of Syndecan-1 in Basal Epidermis Affects Keratinocyte Proliferation and Wound Re-Epithelialization Nkemcho Ojeh, Katri Hiilesvuo,

Comparison of Mouse Matrix Metalloproteinase 13 Expression in Free-Electron Laser and Scalpel Incisions During Wound Healing Nanjun Wu, E. Duco Jansen,

Liren Tang, Shabnam Madani, Harvey Lui, Jerry Shapiro

Bing-Mei Zhu, Yuko Ishida, Gertraud W

The Angiogenesis Inhibitor Vasostatin does not Impair Wound Healing at Tumor- Inhibiting Doses Bernhard Lange-Asschenfeldt, Paula Velasco, Michael Streit,

The Requirement for Perp in Postnatal Viability and Epithelial Integrity Reflects an Intrinsic Role in Stratified Epithelia Michelle R. Marques, Rebecca.

Modulation of IL-10, IL-12, and IFN-γ in the Epidermis of Hairless Mice by UVA (320– 400 nm) and UVB (280–320 nm) Radiation Jie Shen, Shisan Bao, Vivienne.

Keratinocyte-Derived Granulocyte-Macrophage Colony Stimulating Factor Accelerates Wound Healing: Stimulation of Keratinocyte Proliferation, Granulation.

Errata Journal of Investigative Dermatology

Presentation transcript:

The Effects of Cyclooxygenase Isozyme Inhibition onIncisional Wound Healing in Mouse Skin Karin Müller-Decker, Wolfgang Hirschner, Friedrich Marks, Gerhard Fürstenberger Journal of Investigative Dermatology Volume 119, Issue 5, Pages 1189-1195 (November 2002) DOI: 10.1046/j.1523-1747.2002.19501.x Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Gross appearance and progress of wound healing on back skin of mice fed control diet or diet supplemented with 500 p.p.m. valdecoxib or 64 p.p.m. SC-560. Photographs were taken 7 and 28 d after full thickness skin incisions were made. Journal of Investigative Dermatology 2002 119, 1189-1195DOI: (10.1046/j.1523-1747.2002.19501.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Kinetics of re-epithelialization and proliferation in full-thickness incisional skin wounds. Mice were fed control diet (A,B,G,H) or diet containing 500 p.p.m.valdecoxib (C,D,I,J) or 64 p.p.m.SC-560 (E,F,K,L) and analyzed 2 d (G–L), 3 d (A,C,E), and 7 d (B,D,F) after surgery. Specimens were stained with anti-COX-2 antibodies (A–F) or anti-Ki-67 antibodies (G–L). The wound edges are marked with arrows (G,I,K). Epidermis (e), granulation tissue (g), and eschar (es). Original magnification: × 630 (A–F, insert H), × 160 (G–L). (M) Kinetics of proliferation in interfollicular wound epidermis. Ki-67-immunopositive cells were counted in the wound center and the immediate neighboring fields. Values represent mean±SD (**p < 0.005, ***p < 0.0005) according to Student's t test, n=6 sections or 24 microscopic fields (original magnification×160)/group and time point. Journal of Investigative Dermatology 2002 119, 1189-1195DOI: (10.1046/j.1523-1747.2002.19501.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Localization of COX isozymes in incisional wounds by immunohistochemistry with goat anti-COX-1 (A–D) and goat anti-COX-2 (E–H) antibodies. Wound specimens were collected from control animals at various time points: 24 h (A,E,C,G), 7 d (D,H), and 28 d (B,F). Note COX-1 expression in suprabasal cells of interfollicular epidermis (A), dendritic cells (A, insert), endothelial cells (C), and macrophages (D). Note COX-2 expression in basal as well as suprabasal interfollicular keratinocytes (E), the outer root sheath of hair follicles and sebaceous gland epithelium (F), dendritic cells (E, insert), endothelial cells (G), as well as macrophages (H). Original magnification:×630. Journal of Investigative Dermatology 2002 119, 1189-1195DOI: (10.1046/j.1523-1747.2002.19501.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Localization of CD31-positive vessels in incisional wounds from animals fed control diet or diet supplemented with valdecoxib or SC-560. Wound specimens were collected 7 d postsurgery from animals fed control diet (A,A*), valdecoxib diet (B,B*), or SC-560 diet (C,C*), and processed for immunohistochemistry using rat anti-CD31 antibody. Original magnification: ×100 (A–C), × 630 (A*–C*). (D) Vessel density as function of time after incision. Microvessel density was determined in the wound centers as shown under A–C by counting CD31-immunopositive endothelial clusters. Values represent mean ± SD (**p < 0.05), according to Student's t test, n = 6 sections or 12 microscopic fields/group and time point. Journal of Investigative Dermatology 2002 119, 1189-1195DOI: (10.1046/j.1523-1747.2002.19501.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Collagen deposition in incisional wounds and tensile strength after treatment with COX inhibitors. Wound specimens collected 14 d postsurgery from animals fed control diet (A,A*), valdecoxib diet (B,B*), or SC-560 diet (C,C*) were stained using the Masson's trichrome procedure. Original magnification:×100 (A–C),×630 (A*–C*). Measurement of tensile strength of wounded skin was done by means of a tensiometer (D). Values represent mean±SD (n=25 stripes with five animals per group and time point). Indo, indomethacin. Journal of Investigative Dermatology 2002 119, 1189-1195DOI: (10.1046/j.1523-1747.2002.19501.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions