MSI and cutin and suberin composition analyses in reticulated and in-between smooth regions of fruit skin tissues. MSI and cutin and suberin composition.

Slides:



Advertisements
Similar presentations
Average histograms of hemoglobin oxygen saturation in the control and ischemic footpads show the distribution of hemoglobin saturation over all pixels.
Advertisements

Effect of N-WASp glutathione-S-transferase (GST) fusion proteins on force in tracheal muscle strips stimulated with 10−5 M ACh under isometric conditions.
Department of Agronomy
Schematic of the principles of mass spectrometry (MS).
Dual-Luciferase Activation Assay with Potential Targets of ZmbZIP22.
A. Williams, Y. Qian, D. Bear, C.R. Chu  Osteoarthritis and Cartilage 
Volume 100, Issue 3, Pages (February 2011)
Figure 1 Schematic representation of a typical MALDI-MSI workflow
TsNAC1 regulated the growth of T. halophila.
Reticulated regions comprise cells with heavily suberized walls.
Seed set of 2xuief, 4xaly, and 4xaly 2xuief mutants in comparison to that of Col-0. Seed set of 2xuief, 4xaly, and 4xaly 2xuief mutants in comparison to.
Expression of pathogenesis-related (PR) genes in transgenic barley in response to pathogen inoculation. Expression of pathogenesis-related (PR) genes in.
Glc uptake in roots and gene expression in barley seedlings.
Topographic Heterogeneity in Transdermal Transport Revealed by High-Speed Two- Photon Microscopy: Determination of Representative Skin Sample Sizes  Betty.
PD-1 expression on HCC-infiltrating B cells and its clinical significance. PD-1 expression on HCC-infiltrating B cells and its clinical significance. A–H,
Epitope Mapping Performance using a single peptide microarray.
Bottom-up proteomic characterization of MALDI IMS samples.
A, schematic presentation of fetuin-A domains.
Volume 6, Issue 6, Pages (November 2013)
Porphyrin Formation in Actinic Keratosis and Basal Cell Carcinoma after Topical Application of Methyl 5-Aminolevulinate  Even Angell-Petersen, Roar Sørensen,
Expression analysis of MC and JOINTLESS
Validation of ChIP-seq reads.
Structural properties of 2954 OsMADS1-bound sequences in three data sets (intergenic, gene body, and A-tract). Structural properties of 2954 OsMADS1-bound.
Quantitative RT-PCR results of relative gene expression normalized to wild-type (WT) expression levels for the LjMYB14 transgene (A) and genes specific.
Gene expression profiles in tomato pedicels.
RNA-seq results at the SAM
Identification of FD targets by pSUC2::GFP:FD ChIP-seq in Col-0 and ft-10 tsf-1 and pFD::GFP:FD ChIP-seq in fd-2. Identification of FD targets by pSUC2::GFP:FD.
Phosphorylation of FD at T282 modulates flowering time in Arabidopsis.
Expression and Histone H4 Acetylation Levels of MAPK6 and WRKY53 in the HDT701 OX and RNAi Plants after M. oryzae Inoculation. Expression and Histone H4.
Label-free pyQms quantification of metabolites and peptides.
Monitoring the acetylation profile of mitochondrial proteins in SIRT3 KO mice. Monitoring the acetylation profile of mitochondrial proteins in SIRT3 KO.
T7 transcripts increase relative to E. coli transcripts over time.
Summary of differentially expressed genes.
Mitochondria in microsporophylls had higher AOX capacity and protein expression levels than those in microsporangia. Mitochondria in microsporophylls had.
Tapetal mitochondria in wild-type (WT), drp3b, and elm1 mutants.
Mineral content analysis by SEM-EDX.
Cyclic electron flow in hpr1 mutants.
Phenotyping of three hvcen mutants (mat-c. 907, mat-c. 94, mat-c
Inactivation of BOP1, BOP2, TGA1, and TGA4 corrects pny pnf meristem arrest. Inactivation of BOP1, BOP2, TGA1, and TGA4 corrects pny pnf meristem arrest.
Mitochondrial enzymatic activities and root tip respiration in wild type (WT) and mab1-1. Mitochondrial enzymatic activities and root tip respiration in.
Two cycad AOX genes, CrAOX1 and CrAOX2, showing different expression patterns in thermogenic male cones. Two cycad AOX genes, CrAOX1 and CrAOX2, showing.
Numerical analysis of cell morphology of tapetal cells in wild type.
Chlorophyll concentration (conc
Stomatal aperture. Stomatal aperture. A, Stomata of the different lines with (+) and without (-) ABA treatment as photographed during the experiments.
Identification of Primary Target Genes of Phytochrome Signaling
Left: Baseline brain white and gray matter uptake rates of [18F]-FTHA (A) and [11C]-palmitate (B). Left: Baseline brain white and gray matter uptake rates.
Distribution and extent of expression.
Effect of berberine on white adipose tissue mass.
Pie charts (for all the mice together) and bar graph (for individual mouse) showing relative percentage of T cells and B cells present in the Raman spectroscopy–investigated.
Saccharification yields.
Flowering time, biomass, leaf number, and leaf area at flowering of wild-type (WT) and fa plants grown under short- or long-day conditions. Flowering time,
The CACTA Transposon Is Hypermethylated in U-S Plants.
TDIF-Sensitive Xylem Formation in wox4 Mutants
General schematic for MS analysis of ionized microbiological isolates and clinical material. General schematic for MS analysis of ionized microbiological.
Influence of RdDM on DCL4 Transcript Isoform Expression.
Analysis of PHO1 Expression by RT-qPCR in the pru1 Mutant, Complementation Lines (COM5 and COM7), and Wild-type Plants during Pi Starvation. Analysis of.
Natural Variation of GY1 Correlates with Mesocotyl Elongation in Rice Accessions and GY1 Suppresses the Long Mesocotyl and Coleoptile Phenotypes in Kasalath.
Differences in Wax Phenotypes between the Glaucous BL and the Glossy CASL*2BS Wheat Lines. Differences in Wax Phenotypes between the Glaucous BL and the.
Bar graph shows the preventive effect of UV on the development of vasospasm for different irradiation time periods. Bar graph shows the preventive effect.
Light-Dependent Lesions in Ufo1-1 Leaves Have Elevated Sucrose and Starch Levels. Light-Dependent Lesions in Ufo1-1 Leaves Have Elevated Sucrose and Starch.
Relative Protein Quantification of CcmK4, RbcS, and CcmM in the Carboxysome under Different CO2 Levels and Light Intensities using Confocal Microscopy.
l-2HG is elevated in RCC tumors and cell lines.
Cell Sorting of Induced SE-like Cells Using SEOR1pro:SEOR1-YFP.
Distribution of diterpenoids in Stevia leaf tissues.
ROS Generation, Defense Gene Expression, and Histone Acetylation Levels in the HDT701 OX and RNAi Plants.(A) ROS level in HDT701 RNAi plants after flg22.
NRG2 Affects Nitrate Accumulation and Uptake
Fig. 1 Spatial and cartilage depth-related distributions of deamidated proteins in human adult cartilage. Spatial and cartilage depth-related distributions.
Kros of Single grf Knockout Mutants.
PIF3 Inhibits the Binding of TCP4 to the Promoters of SAUR Genes and Represses Their Expression. PIF3 Inhibits the Binding of TCP4 to the Promoters of.
Binding of TCP4-Like Proteins to the SAUR Promoters Is Enhanced by Light Signal or the Lack of PIFs. (A) and (B) Light increases the binding of TCP4 to.
Presentation transcript:

MSI and cutin and suberin composition analyses in reticulated and in-between smooth regions of fruit skin tissues. MSI and cutin and suberin composition analyses in reticulated and in-between smooth regions of fruit skin tissues. A, Light microscopy analysis of fruit skin samples analyzed using MALDI-MSI. B, MALDI-MSI showing the spatial distribution of ferulic acid (195.065 ± 0.001 mass-to-charge ratio [m/z]) located in the reticulated areas of reticulated skin samples but absent in the smooth skin samples. Dashed white lines represent skin surface areas analyzed by MALDI-MSI. Bars in A and B = 1 mm. C, Manual dissection of reticulated regions and the smooth regions in-between reticulated ones from cv Sharlyn fruit skin. D, Total cutin and suberin contents in skin tissues dissected as shown in C. E, Levels of individual cutin and suberin monomers in skin tissues dissected as in C. Cutin and suberin profiles in D and E were analyzed by GC-MS; y axes represent relative peak areas following normalization to a C32-alkane internal standard. Data represent means ± se of three biological replicates each per sample (generated from a pool of skin tissues from four different fruit). Significance was calculated according to Student’s t test: *, P < 0.05; **, P < 0.001; and ***, P < 0.0001. Hagai Cohen et al. Plant Physiol. 2019;179:1486-1501 ©2019 by American Society of Plant Biologists