Creating a Buzz about Macrophages: The Fly as an In Vivo Model for Studying Immune Cell Behavior  Helen Weavers, Will Wood  Developmental Cell  Volume 38, Issue 2, Pages 129-132 (July 2016) DOI: 10.1016/j.devcel.2016.07.002 Copyright © 2016 Elsevier Inc. Terms and Conditions

Figure 1 The Fly Is a Powerful In Vivo Model to Study Diverse Macrophage Functions Drosophila macrophages have been used to study multiple functions in vivo, ranging from their response to various cues (including those produced by apoptotic corpses, damaged tissue, pathogens, and transformed cells) to communicating with other tissues to help direct tissue morphogenesis or maintain stem cell niches. They also sense and respond to each other, exhibiting contact inhibition of locomotion during development. These impressive multi-tasking cells have also recently been shown to play a role in mediating dietary effects on lifespan. Developmental Cell 2016 38, 129-132DOI: (10.1016/j.devcel.2016.07.002) Copyright © 2016 Elsevier Inc. Terms and Conditions

Figure 2 All Four Fly Life Stages Offer Opportunities for Studying Macrophage Behavior All stages of Drosophila development have been used to study different aspects of macrophage behavior. Embryonic macrophages (red) are extremely long-lived cells that are derived from the embryonic head mesoderm and distribute throughout the embryo by a series of stereotypical migrations. The embryo lends itself to live imaging many aspects of macrophage behavior, from their roles during tissue morphogenesis to the response to wounds and/or infection. As development proceeds to larval stages, the embryonic macrophages populate the larva and can be recruited to wounds or tumors. A second population of macrophages (in green) develops in the larval lymph gland and is released during pupal development. These macrophages also show a strong inflammatory response to wounds made in the pupal wing disc and ultimately populate the adult, where they have recently been shown to mediate dietary effects on lifespan, as well as to maintain stem cell niches. Developmental Cell 2016 38, 129-132DOI: (10.1016/j.devcel.2016.07.002) Copyright © 2016 Elsevier Inc. Terms and Conditions