Mast Cells Are Required for Full Expression of Allergen/SEB-Induced Skin Inflammation Tomoaki Ando, Kenji Matsumoto, Siavash Namiranian, Hirotaka Yamashita,

Slides:

Advertisements

Similar presentations

IL-17A as an Inducer for Th2 Immune Responses in Murine Atopic Dermatitis Models Saeko Nakajima, Akihiko Kitoh, Gyohei Egawa, Yohei Natsuaki, Satoshi.

Advertisements

The Tumor Necrosis Factor Superfamily Molecule LIGHT Promotes Keratinocyte Activity and Skin Fibrosis Rana Herro, Ricardo Da S. Antunes, Amelia R. Aguilera,

Complex 2B4 Regulation of Mast Cells and Eosinophils in Murine Allergic Inflammation Moran Elishmereni, Nanna Fyhrquist, Roopesh Singh Gangwar, Sari Lehtimäki,

Loss of Extracellular Superoxide Dismutase Induces Severe IL-23-Mediated Skin Inflammation in Mice Yun Sang Lee, In-Su Cheon, Byung-Hak Kim, Myung-Ja.

Integrin α2β1 Is Required for Regulation of Murine Wound Angiogenesis but Is Dispensable for Reepithelialization Manon C. Zweers, Jeffrey M. Davidson,

Atopic March from Atopic Dermatitis to Asthma-Like Lesions in NC/Nga Mice Is Accelerated or Aggravated by Neutralization of Stratum Corneum but Partially.

TSLP Induces Mast Cell Development and Aggravates Allergic Reactions through the Activation of MDM2 and STAT6 Na-Ra Han, Hyun-A Oh, Sun-Young Nam, Phil-Dong.

Unprocessed Interleukin-36α Regulates Psoriasis-Like Skin Inflammation in Cooperation With Interleukin-1 Katelynn A. Milora, Hangfei Fu, Ornella Dubaz,

Loss of Keratin K2 Expression Causes Aberrant Aggregation of K10, Hyperkeratosis, and Inflammation Heinz Fischer, Lutz Langbein, Julia Reichelt, Silke.

Volume 35, Issue 4, Pages (October 2011)

Erdr1 Attenuates Dermatophagoides farina Body Extract-Induced Atopic Dermatitis in NC/Nga Mice Kyung Eun Kim, Myung Jin Jung, Younkyung Houh, Tae Sung.

UVB-Induced Skin Inflammation and Cutaneous Tissue Injury Is Dependent on the MHC Class I–Like Protein, CD1d Stephan Ryser, Marlène Schuppli, Beatrice.

Α(1,3) Fucosyltransferases IV and VII Are Essential for the Initial Recruitment of Basophils in Chronic Allergic Inflammation Kazumi Saeki, Takahiro.

Critical Role for Mast Cell Stat5 Activity in Skin Inflammation

Topical ROR Inverse Agonists Suppress Inflammation in Mouse Models of Atopic Dermatitis and Acute Irritant Dermatitis Jun Dai, Min-Kyung Choo, Jin Mo.

Type I IL-1 Receptor Mediates IL-1 and Intracellular IL-1 Receptor Antagonist Effects in Skin Inflammation Gaby Palmer, Dominique Talabot-Ayer, Gürkan.

Trim32 Deficiency Enhances Th2 Immunity and Predisposes to Features of Atopic Dermatitis Yuangang Liu, Zhiping Wang, Rachel De La Torre, Ashley Barling,

Oral Administration of Poly-γ-Glutamate Ameliorates Atopic Dermatitis in Nc/Nga Mice by Suppressing Th2-Biased Immune Response and Production of IL-17A

Conditional PDK1 Ablation Promotes Epidermal and T-Cell-Mediated Dysfunctions Leading to Inflammatory Skin Disease Minjun Yu, David M. Owens, Sankar.

Induction of Thymic Stromal Lymphopoietin Expression in Keratinocytes Is Necessary for Generating an Atopic Dermatitis upon Application of the Active.

IL-17A as an Inducer for Th2 Immune Responses in Murine Atopic Dermatitis Models Saeko Nakajima, Akihiko Kitoh, Gyohei Egawa, Yohei Natsuaki, Satoshi.

IL-27 Activates Th1-Mediated Responses in Imiquimod-Induced Psoriasis-Like Skin Lesions Sayaka Shibata, Yayoi Tada, Yoshihide Asano, Koichi Yanaba, Makoto.

Holly Anderton, James A. Rickard, George A

Aspartame Attenuates 2, 4-Dinitrofluorobenzene-Induced Atopic Dermatitis–Like Clinical Symptoms in NC/Nga Mice Gun-Dong Kim, Yong Seek Park, Hyun-Jong.

Functional Beta2-Integrins Restrict Skin Inflammation In Vivo

IL-23 from Langerhans Cells Is Required for the Development of Imiquimod-Induced Psoriasis-Like Dermatitis by Induction of IL-17A-Producing γδ T Cells

Ganna Bilousova, Jiang Chen, Dennis R. Roop

Caspase-14-Deficient Mice Are More Prone to the Development of Parakeratosis Esther Hoste, Geertrui Denecker, Barbara Gilbert, Filip Van Nieuwerburgh,

Jamie Rosen, Angelo Landriscina, Allison Kutner, Brandon L

The Absence of a Microbiota Enhances TSLP Expression in Mice with Defective Skin Barrier but Does Not Affect the Severity of their Allergic Inflammation

Herpes Simplex Virus Associated Erythema Multiforme (HAEM) is Mechanistically Distinct from Drug-Induced Erythema Multiforme: Interferon-γ is Expressed.

Exposure to food allergens through inflamed skin promotes intestinal food allergy through the thymic stromal lymphopoietin–basophil axis Mario Noti,

ST2 Regulates Allergic Airway Inflammation and T-Cell Polarization in Epicutaneously Sensitized Mice Terhi Savinko, Piia Karisola, Sari Lehtimäki, Anna-Mari.

Capsiate Inhibits DNFB-Induced Atopic Dermatitis in NC/Nga Mice through Mast Cell and CD4+ T-Cell Inactivation Ji H. Lee, Yun S. Lee, Eun-Jung Lee, Ji.

Integrin β6-Deficient Mice Show Enhanced Keratinocyte Proliferation and Retarded Hair Follicle Regression after Depilation Yanshuang Xie, Kevin J. McElwee,

Aggravated TSLP-Induced Atopic Dermatitis in Mice Lacking Dicer in Adult Skin Keratinocytes Pierre Hener, Laetitia Friedmann, Daniel Metzger, Pierre.

Development of Atopic Dermatitis in Mice Transgenic for Human Apolipoprotein C1 Lex Nagelkerken, Perry Verzaal, Tonny Lagerweij, Carla Persoon-Deen, Jimmy.

Characterization of the Progressive Skin Disease and Inflammatory Cell Infiltrate in Mice with Inhibited NF-κB Signaling Max van Hogerlinden, Barbro.

Chronic Mouse Model of TMA-Induced Contact Hypersensitivity

Mast Cells Play a Key Role in Host Defense against Herpes Simplex Virus Infection through TNF-α and IL-6 Production Rui Aoki, Tatsuyoshi Kawamura, Fumi.

MAPKAPK-2 Signaling Is Critical for Cutaneous Wound Healing

Epidermal RelA Specifically Restricts Contact Allergen–Induced Inflammation and Apoptosis in Skin Snehlata Kumari, Benjamin Herzberg, Ruth Pofahl, Thomas.

IL-33 and ST2 in Atopic Dermatitis: Expression Profiles and Modulation by Triggering Factors Terhi Savinko, Sampsa Matikainen, Ulpu Saarialho-Kere, Maili.

Critical Role for Mast Cell Stat5 Activity in Skin Inflammation

Keratinocyte-Specific Deletion of the Receptor RAGE Modulates the Kinetics of Skin Inflammation In Vivo Julia S. Leibold, Astrid Riehl, Jan Hettinger,

Suppression of E-Cadherin Function Drives the Early Stages of Ras-Induced Squamous Cell Carcinoma through Upregulation of FAK and Src Addy Alt-Holland,

Chiara Giacomassi, Norzawani Buang, Guang Sheng Ling, Greg Crawford, H

Judith A. Mack, Edward V. Maytin Journal of Investigative Dermatology

The Differential Role of L-Selectin and ICAM-1 in Th1-Type and Th2-Type Contact Hypersensitivity Asako Ogawa, Ayumi Yoshizaki, Koichi Yanaba, Fumihide.

Semaphorin3A Alleviates Skin Lesions and Scratching Behavior in NC/Nga Mice, an Atopic Dermatitis Model Junko Yamaguchi, Fumio Nakamura, Michiko Aihara,

Mechanisms of Contact Sensitization Offer Insights into the Role of Barrier Defects vs. Intrinsic Immune Abnormalities as Drivers of Atopic Dermatitis

Joanna Shepherd, Matthew C. Little, Martin J.H. Nicklin

Epicutaneous Natural Rubber Latex Sensitization Induces T Helper 2-Type Dermatitis and Strong Prohevein-Specific IgE Response Maili Lehto, Minna Koivuluhta,

Bing-Mei Zhu, Yuko Ishida, Gertraud W

Increased Severity of Bleomycin-Induced Skin Fibrosis in Mice with Leukocyte-Specific Protein 1 Deficiency JianFei Wang, Haiyan Jiao, Tara L. Stewart,

Lack of Galanin Receptor 3 Alleviates Psoriasis by Altering Vascularization, Immune Cell Infiltration, and Cytokine Expression Felix Locker, Silvia Vidali,

Β-Arrestin 2 Inhibits Proinflammatory Chemokine Production and Attenuates Contact Allergic Inflammation in the Skin Evelyn Gaffal, Mira Jakobs, Nicole.

Minutes of the Board of Directors Meeting

Epidermal Inactivation of the Glucocorticoid Receptor Triggers Skin Barrier Defects and Cutaneous Inflammation Lisa M. Sevilla, Víctor Latorre, Ana Sanchis,

IL-23-Mediated Epidermal Hyperplasia Is Dependent on IL-6

Society for Investigative Dermatology 2010 Meeting Minutes

Oral Administration of Poly-γ-Glutamate Ameliorates Atopic Dermatitis in Nc/Nga Mice by Suppressing Th2-Biased Immune Response and Production of IL-17A

IgE Basement Membrane Zone Antibodies Induce Eosinophil Infiltration and Histological Blisters in Engrafted Human Skin on SCID Mice John J. Zone, Ted.

Smad3 Signal Transducer Regulates Skin Inflammation and Specific IgE Response in Murine Model of Atopic Dermatitis Minna Anthoni, Guoying Wang, Chuxia.

The Absence of uPAR Is Associated with the Progression of Dermal Fibrosis Yosuke Kanno, Aki Kaneiwa, Misato Minamida, Miho Kanno, Kanji Tomogane, Koji.

Tannic Acid and Quercetin Display a Therapeutic Effect in Atopic Dermatitis via Suppression of Angiogenesis and TARC Expression in Nc/Nga Mice Min Kyung.

Transgenic Expression of Interleukin-13 in the Skin Induces a Pruritic Dermatitis and Skin Remodeling Tao Zheng, Min H. Oh, Sun Y. Oh, John T. Schroeder,

Ascorbic Acid Deficiency Leads to Epidermal Atrophy and UVB-Induced Skin Pigmentation in SMP30/GNL Knockout Hairless Mice Yasunori Sato, Koji Y. Arai,

Par2 Inactivation Inhibits Early Production of TSLP, but Not Cutaneous Inflammation, in Netherton Syndrome Adult Mouse Model Anaïs Briot, Matthieu Lacroix,

Induction of Matrix Metalloproteinase-9 in Keratinocytes by Histamine

Presentation transcript:

Mast Cells Are Required for Full Expression of Allergen/SEB-Induced Skin Inflammation Tomoaki Ando, Kenji Matsumoto, Siavash Namiranian, Hirotaka Yamashita, Haley Glatthorn, Miho Kimura, Brandon R. Dolan, James J. Lee, Stephen J. Galli, Yuko Kawakami, Colin Jamora, Toshiaki Kawakami Journal of Investigative Dermatology Volume 133, Issue 12, Pages 2695-2705 (December 2013) DOI: 10.1038/jid.2013.250 Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 T cells, but not B cells, are indispensable for maximal skin inflammation. Dermatitis induction by epicutaneous applications of Dermatophagoides farinae extract (Der f) and Staphylococcal enterotoxin B (SEB) was performed as described in the Materials and Methods. Each symbol represents one mouse. (a) Clinical skin scores. (b) Hematoxylin and eosin (H&E) staining of naive (upper rows) and lesional (lower rows) skin tissues. Bar=200μm. (c) Thicknesses of epidermis, dermis, and total skin (epidermis+dermis) layers, as measured in H&E-stained tissues. (d) Relationships between clinical scores and skin layer thicknesses. Linear regression lines are shown. *P<0.05, **P<0.01, ***P<0.001, and ****P<0.0001. AD, atopic dermatitis; NS, not significant; WT, wild type. Journal of Investigative Dermatology 2013 133, 2695-2705DOI: (10.1038/jid.2013.250) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Eosinophils are dispensable for allergen-induced skin inflammation. (a, b) Eosinophils stained with Congo red were enumerated in the skin sections derived from experiments shown in Figure 1. Each symbol represents one mouse. There was no significant correlation between eosinophil numbers and clinical scores. (c, d) Dermatitis induction by epicutaneous applications of Dermatophagoides farinae extract and Staphylococcal enterotoxin B (SEB) was performed on eosinophil-deficient (c) PHIL and (d) ΔdblGATA mice. Clinical scores are shown. AD, atopic dermatitis; HPF, high power field; NS, not significant; WT, wild type. Journal of Investigative Dermatology 2013 133, 2695-2705DOI: (10.1038/jid.2013.250) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Mast cells are indispensable for maximal skin inflammation. (a) Mast cell–deficient KitW-sh/W-sh mice exhibited lower clinical scores than wild-type (WT) mice. The scores similar to WT mice were restored by engraftment of bone marrow–derived mast cells (BMMCs; W-sh+BMMC). (b) Hematoxylin and eosin (H&E) staining of naive and lesional skin tissues. Enlarged images of the areas indicated by rectangles are shown below. Bar=200μm. (c) Thicknesses of epidermis, dermis, and total skin layers. (d) Immunofluorescence microscopy was performed on naive and lesional skin tissues. Numbers of (e) eosinophils and (f) neutrophils before and after atopic dermatitis (AD) induction. *P<0.05, **P<0.01, and ***P<0.001. Col, collagen; Ecad, E-cadherin; HPF, high power field; K, keratin; Lor, loricrin. Journal of Investigative Dermatology 2013 133, 2695-2705DOI: (10.1038/jid.2013.250) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 FcεRI, but not tumor necrosis factor-α (TNF-α), is required for maximal skin inflammation. Dermatophagoides farinae extract/Staphylococcal enterotoxin B (Der f/SEB) induction experiments were performed on (a–e) FcεRIα−/- and (f) TNF-α−/- mice. (a, f) Clinical skin scores. (b–d) Thicknesses of epidermis, dermis, and total skin layers, and (e) inflammatory cell infiltration for these mice are also shown. (b) Hematoxylin and eosin (H&E) staining and (d) immunofluorescence microscopy were performed on naive and lesional skin tissues in FcεRIα−/- mice. *P<0.05. AD, atopic dermatitis; Col, collagen; Ecad, E-cadherin; HPF, high power field; K, keratin; Lor, loricrin; WT, wild type. Journal of Investigative Dermatology 2013 133, 2695-2705DOI: (10.1038/jid.2013.250) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 The T helper type 2 (Th2)–promoting cytokine thymic stromal lymphopoietin (TSLP) contributes to skin inflammation. (a) Expression of TSLP (red) before (upper) and after (lower) dermatitis induction with Dermatophagoides farinae extract/Staphylococcal enterotoxin B (Der f/SEB) in wild-type (WT) mice was revealed by immunofluorescence microscopy. Also shown are enlarged images of the areas indicated by rectangles as well as negative control without primary antibody. Bar=100μm. (b–f) Der f/SEB induction experiments were performed on TSLPR−/- mice. (b) Clinical skin scores, (c, d) thicknesses of epidermis, dermis, and total skin layers, and (e) inflammatory cell infiltration are shown. (c) Hematoxylin and eosin (H&E) staining and (f) immunofluorescence microscopy were performed on naive and lesional skin tissues in TSLPR−/- mice. **P<0.01 and ***P<0.001 by Student’s t-test. AD, atopic dermatitis; Col, collagen; Ecad, E-cadherin; HPF, high power field; K, keratin; Lor, loricrin. Journal of Investigative Dermatology 2013 133, 2695-2705DOI: (10.1038/jid.2013.250) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions