Characterization of an Autoantigen Associated With Chronic Ulcerative Stomatitis: The CUSP Autoantigen is a Member of the p53 Family1 Lela A. Lee, Patrick.

Slides:

Advertisements

Similar presentations

Expression of Human Macrophage Metalloelastase (MMP-12) by Tumor Cells in Skin Cancer Erja Kerkelä, Risto Ala-aho, Leila Jeskanen, Oona Rechardt, Reidar.

Advertisements

Hair Keratin Associated Proteins: Characterization of a Second High Sulfur KAP Gene Domain on Human Chromosome 211 Michael A. Rogers, Hermelita Winter,

Characterization of the Human Hair Shaft Cuticle–Specific Keratin-Associated Protein 10 Family Hiroki Fujikawa, Atsushi Fujimoto, Muhammad Farooq, Masaaki.

Hair Keratin Associated Proteins: Characterization of a Second High Sulfur KAP Gene Domain on Human Chromosome 211 Michael A. Rogers, Hermelita Winter,

Skin-Specific Expression of ank-393, a Novel Ankyrin-3 Splice Variant

Abdi Ghaffari, Ruhangiz T. Kilani, Aziz Ghahary

Matrix Metalloproteinase 9 Expression is Coordinately Modulated by the KRE-M9 and 12-O-Tetradecanoyl-Phorbol-13-Acetate Responsive Elements Takashi Kobayashi,

Regulation of MAPK Activation, AP-1 Transcription Factor Expression and Keratinocyte Differentiation in Wounded Fetal Skin Samantha Gangnuss, Allison.

Characterization of Mouse Frizzled-3 Expression in Hair Follicle Development and Identification of the Human Homolog in Keratinocytes Betsy S. Hung,

BM-40(Osteonectin, SPARC) Is Expressed Both in the Epidermal and in the Dermal Compartment of Adult Human Skin Nicholas Hunzelmann, Martin Hafner, Sabine.

Bullous Pemphigoid Sera that Contain Antibodies to BPAg2 also Contain Antibodies to LABD97 that Recognize Epitopes Distal to the NC16A Domain Conleth.

Expression of Type XVI Collagen in Human Skin Fibroblasts: Enhanced Expression in Fibrotic Skin Diseases Atsushi Akagi, Shingo Tajima, Yutaka Nagai

Differential Expression of a Novel Gene in Response to hsp27 and Cell Differentiation in Human Keratinocytes Mojgan Hell-Pourmojib, Peter Neuner, Robert.

Retinoic Acid Inhibits Downregulation of ΔNp63α Expression During Terminal Differentiation of Human Primary Keratinocytes Casimir Bamberger, Hartwig.

Rat mesangial α-endosulfine

Rose-Anne Romano, Barbara Birkaya, Satrajit Sinha

Volume 16, Issue 5, Pages (December 2004)

A Novel Mouse Gene, Sh3yl1, is Expressed in the Anagen Hair Follicle

Multiple PKCδ Tyrosine Residues Are Required for PKCδ-Dependent Activation of Involucrin Expression—a Key Role of PKCδ-Y311 Ling Zhu, Chaya Brodie, Sivaprakasam.

Transcriptional Control of the Mouse Col7a1 Gene in Keratinocytes: Basal and Transforming Growth Factor-β Regulated Expression Michael Naso, Jouni Uitto,

Molecular Cloning and Expression of Human Keratinocyte Proline-Rich Protein (hKPRP), an Epidermal Marker Isolated from Calcium-Induced Differentiating.

Claudia D. Andl, John R. Stanley Journal of Investigative Dermatology

C-Terminus of Desmoyokin/AHNAK Protein is Responsible for its Translocation Between the Nucleus and Cytoplasm Zhuxiang Nie, Takashi Hashimoto Journal.

Novel Homozygous and Compound Heterozygous COL17A1 Mutations Associated with Junctional Epidermolysis Bullosa Michaela Floeth, Heike Schäcke, Nadja Hammami-Hauasli,

Characterization of Group X Phospholipase A2 as the Major Enzyme Secreted by Human Keratinocytes and its Regulation by the Phorbol Ester TPA Gérard Lambeau,

Paraneoplastic Pemphigus Sera React Strongly with Multiple Epitopes on the Various Regions of Envoplakin and Periplakin, Except for the C-Terminal Homologous.

Autoantibodies in a Subgroup of Patients with Linear IgA Disease React with the NC16A Domain of BP1801 Detlef Zillikens, Karin Herzele, Matthias Georgi,

H. Randolph Byers, Mina Yaar, Mark S. Eller, Nicole L

Erythropoietin stimulates proliferation of human renal carcinoma cells

Cloning and Characterization of the Expression Pattern of a Novel Splice Product MIA (Splice) of Malignant Melanoma-derived Growth-inhibiting Activity.

Olga M. Mazina, Marjorie A. Phillips, Trevor Williams, Carol A

Characterization of Kdap, A Protein Secreted by Keratinocytes

Noritaka Oyama, Keiji Iwatsuki, Yoshimi Homma, Fumio Kaneko

Naoko Kanda, Shinichi Watanabe Journal of Investigative Dermatology

Volume 15, Issue 5, Pages (November 2001)

Rapid Decay of α6 Integrin Caused by a Mis-Sense Mutation in the Propeller Domain Results in Severe Junctional Epidermolysis Bullosa with Pyloric Atresia

Functional Analysis of the Profilaggrin N-Terminal Peptide: Identification of Domains that Regulate Nuclear and Cytoplasmic Distribution David J. Pearton,

Aberrant Nuclear Trafficking of La Protein Leads to Disordered Processing of Associated Precursor tRNAs Robert V Intine, Miroslav Dundr, Tom Misteli,

Halofuginone, an Inhibitor of Type-I Collagen Synthesis and Skin Sclerosis, Blocks Transforming-Growth-Factor-β-Mediated Smad3 Activation in Fibroblasts

Characterization of the Human Hair Keratin–Associated Protein 2 (KRTAP2) Gene Family Hiroki Fujikawa, Atsushi Fujimoto, Muhammad Farooq, Masaaki Ito,

Deletion of the Cytoplasmatic Domain of BP180/Collagen XVII Causes a Phenotype with Predominant Features of Epidermolysis Bullosa Simplex Marcel Huber,

Autoantibodies to Bullous Pemphigoid Antigen 180 Induce Dermal–Epidermal Separation in Cryosections of Human Skin Cassian Sitaru, Enno Schmidt, Steffen.

Characterization of Keratinocyte Differentiation Induced by Ascorbic Acid: Protein Kinase C Involvement and Vitamin C Homeostasis1 Isabella Savini, Antonello.

Regulation of the Expression of Peptidylarginine Deiminase Type II Gene (PADI2) in Human Keratinocytes Involves Sp1 and Sp3 Transcription Factors Sijun.

Volume 6, Issue 3, Pages (September 2000)

Keratinocytes Express the CD146 (Muc18/S-Endo) Antigen in Tissue Culture and During Inflammatory Skin Diseases1 Wolfgang Weninger, Michael Rendl, Michael.

Characterization of New Members of the Human Type II Keratin Gene Family and a General Evaluation of the Keratin Gene Domain on Chromosome 12q13.13 Michael.

Bikunin, a Serine Protease Inhibitor, is Present on the Cell Boundary of Epidermis Cui Chang-Yi, Yoshinori Aragane, Akira Maeda, Piao Yu-Lan, Masae Takahashi,

Rab3a and SNARE Proteins: Potential Regulators of Melanosome Movement

The Members of the Plakin Family of Proteins Recognized by Paraneoplastic Pemphigus Antibodies Include Periplakin My G. Mahoney, Sirpa Aho, Jouni Uitto,

Analyses of the Effects That Disease-Causing Missense Mutations Have on the Structure and Function of the Winged-Helix Protein FOXC1 Ramsey A. Saleem,

Cloning of a novel gene in the human kidney homologous to rat munc13s: Its potential role in diabetic nephropathy Yong Song, Menachem Ailenberg, Mel.

Molecular Cloning and Tissue Expression of the Murine Analog to Human Stratum Corneum Chymotryptic Enzyme Assar Bäckman, Lennart Hansson Journal of.

Compound Heterozygosity for Novel Splice Site Mutations in the BPAG2/COL17A1 Gene Underlies Generalized Atrophic Benign Epidermolysis Bullosa Leena Pulkkinen,

A Novel Gene Expressed in Human Keratinocytes with Long-Term In Vitro Growth Potential is Required for Cell Growth Laure Aurelian, Cynthia C. Smith,

IgG Autoantibodies from Bullous Pemphigoid (BP) Patients Bind Antigenic Sites on Both the Extracellular and the Intracellular Domains of the BP Antigen.

Anti-Alpha-2-Macroglobulin-Like-1 Autoantibodies Are Detected Frequently and May Be Pathogenic in Paraneoplastic Pemphigus Sanae Numata, Kwesi Teye,

The Effect of LXR Activators on AP-1 Proteins in Keratinocytes

Expression of FcRn, the MHC Class I-Related Receptor for IgG, in Human Keratinocytes Karla Cauza, Gabriele Hinterhuber, Ruth Dingelmaier-Hovorka, Karin.

Lawrence M. Pfeffer, Andrzej T. Slominski

Autoantibody in Mucous Membrane Pemphigoid Binds to an Intracellular Epitope on Human β4 Integrin and Causes Basement Membrane Zone Separation in Oral.

Autoantibodies to BP180 Associated with Bullous Pemphigoid Release Interleukin-6 and Interleukin-8 from Cultured Human Keratinocytes Enno Schmidt, Stanislaus.

IgG Autoantibodies from Bullous Pemphigoid Patients Recognize Multiple Antigenic Reactive Sites Located Predominantly Within the B and C Subdomains of.

Defining the Regulatory Elements in the Proximal Promoter of ΔNp63 in Keratinocytes: Potential Roles for Sp1/Sp3, NF-Y, and p63 Rose-Anne Romano, Barbara.

Ligation of the β4 Integrin Triggers Adhesion Behavior of Human Keratinocytes by an “Inside-out” Mechanism Stefan Kippenberger, Stefan Loitsch, Jutta.

Matthias Lüftl, Martin Röcken, Gerd Plewig, Klaus Degitz

Naoko Kanda, Shinichi Watanabe Journal of Investigative Dermatology

Bcl-2 and bcl-xL Antisense Oligonucleotides Induce Apoptosis in Melanoma Cells of Different Clinical Stages Robert A. Olie, Christoph Hafner, Renzo Küttel,

Hepatocyte Growth Factor/Scatter Factor (HGF/SF) Induces Vascular Permeability Factor (VPF/VEGF) Expression by Cultured Keratinocytes Jens Gille, Mona.

Effect of Healing on the Expression of Transforming Growth Factor βs and their Receptors in Chronic Venous Leg Ulcers Allison J. Cowin, Nicholas Hatzirodos,

Presentation transcript:

Characterization of an Autoantigen Associated With Chronic Ulcerative Stomatitis: The CUSP Autoantigen is a Member of the p53 Family1 Lela A. Lee, Patrick Walsh, Cheryl A. Prater, Lih-Jen Su, Angela Marchbank, Timothy B. Egbert, Robert P. Dellavalle, Ira N. Targoff, Kenneth M. Kaufman, Tadeusz P. Chorzelski, Stephania Jablonska Journal of Investigative Dermatology Volume 113, Issue 2, Pages 146-151 (August 1999) DOI: 10.1046/j.1523-1747.1999.00651.x Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Cellular location of autoantibodies in chronic ulcerative stomatitis. Cultured normal human keratinocytes were incubated with serum from a patient with chronic ulcerative stomatitis. Antibody binding is identified using a fluoresceinated probe. There is particulate staining of keratinocyte nuclei. Scale bar: 10 μm. Journal of Investigative Dermatology 1999 113, 146-151DOI: (10.1046/j.1523-1747.1999.00651.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 CUS antibodies bind a 70 kD keratinocyte protein. (a) Immunoblot with HeLa cell extract (left side) and cultured keratinocyte extract (right side). Reactivity of cell extracts with control normal sera is shown in lanes 1 and 2. Reactivity with control dermatomyositis and lupus sera is shown in lanes 3–5. Reactivity with CUS sera is shown in lanes 6–10. A different serum is used for each lane. The CUS sera bind a protein migrating at 70 kDa (arrow) present in the keratinocyte extract but not the HeLa cell extract. (b) Immunoprecipitation of proteins from HeLa cells (left side) and cultured keratinocytes (right side). Proteins precipitated by normal sera are shown in lanes 1 and 2, and proteins precipitated by dermatomyositis and lupus sera are shown in lanes 3–5. Proteins precipitated by CUS sera are shown in lanes 6–10. The notable finding is the presence of a protein at 70 kDa precipitated from keratinocytes but not HeLa cells by CUS antibodies. Journal of Investigative Dermatology 1999 113, 146-151DOI: (10.1046/j.1523-1747.1999.00651.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Expression of CUSP protein in COS cells. Following polycationic transfection of full-length CUSP, CUSP expression was identified using CUS serum and an immunofluorescence technique. The transfected COS cells show nuclear fluorescence. Scale bar: 10 μm. Journal of Investigative Dermatology 1999 113, 146-151DOI: (10.1046/j.1523-1747.1999.00651.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Predicted amino acid sequence of CUSP and its comparison with p51A, p51B, p73α, p73β, and human p53. The cDNA sequence of CUSP is available through GenBank, accession number AF091627. The predicted amino acid sequences of p51A, p51B, p73α, p73β, and p53 were transcribed directly from GenBank. The sequence alignment was performed by the ClustalW Multiple Sequence Alignment program. The boxed areas represent the transactivation (I) domain, the conserved regions (II–V) of the sequence-specific DNA binding domain, and the oligomerization (OLIG) domain. Journal of Investigative Dermatology 1999 113, 146-151DOI: (10.1046/j.1523-1747.1999.00651.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Diagram of the relationship of CUSP to p40, p51A, p51B, and rat KET. Regions where the sequences are virtually identical are depicted as identically filled boxes. The approximate locations of the transactivation, DNA binding, and oligomerization domains are noted as TA, DNA, and OLIG, respectively. Rat KET, the originally described gene, is shown in the middle, with the human homologs of KET above and below. Rat KET and p51B are virtually identical. CUSP/p73L and p40 have a different 5′ sequence than rat KET, with p40 having a truncated 3′ region. CUSP/p73L have the same 3′ sequence as rat KET and p51B. p51A differs from p51B and rat KET at the 3′ end. Journal of Investigative Dermatology 1999 113, 146-151DOI: (10.1046/j.1523-1747.1999.00651.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 6 Expression of CUSP mRNA in cultured cells. Shown is a northern blot hybridized to a CUSP probe in the upper panel (a) and to an actin probe in the lower panel (b). The lanes contain RNA from the following cultured cellslane 1, A431 epithelial carcinoma cells; lane 2, COS monkey kidney cells; lane 3, HaCaT transformed keratinocytes; lane 4, melanoma cells; and lane 5, normal human keratinocytes. The 4.7 kb CUSP transcript (lower arrow) is readily identified in the keratinocyte-derived cells. In lane 5, there is also a weak band at ≈5.9 kb (upper arrow). Journal of Investigative Dermatology 1999 113, 146-151DOI: (10.1046/j.1523-1747.1999.00651.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions