Vitamin D prevents articular cartilage erosion by regulating collagen II turnover through TGF-β1 in ovariectomized rats S. Li, G. Niu, Y. Wu, G. Du,

Slides:

Advertisements

Similar presentations

Pro-inflammatory stimulation of meniscus cells increases production of matrix metalloproteinases and additional catabolic factors involved in osteoarthritis.

Advertisements

B. Bai, Y. Li Osteoarthritis and Cartilage

Early genetic restoration of lubricin expression in transgenic mice mitigates chondrocyte peroxynitrite release and caspase-3 activation K.M. Larson,

Effects of pleiotrophin, a heparin-binding growth factor, on human primary and immortalized chondrocytes Dr. T. Pufe, Ph.D., G. Groth, M.B. Goldring,

Celecoxib exerts protective effects on extracellular matrix metabolism of mandibular condylar chondrocytes under excessive mechanical stress S.-C. Su,

Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is increased in osteoarthritis and regulates chondrocyte catabolic and anabolic activities

GDF5 reduces MMP13 expression in human chondrocytes via DKK1 mediated canonical Wnt signaling inhibition L. Enochson, J. Stenberg, M. Brittberg, A. Lindahl

L. J. Sandell, Ph. D. , X. Xing, M. D. , C. Franz, M. A. , S

Regulation of mesenchymal stem cell chondrogenesis by glucose through protein kinase C/transforming growth factor signaling T.-L. Tsai, P.A. Manner,

MAPKs are essential upstream signaling pathways in proteolytic cartilage degradation – divergence in pathways leading to aggrecanase and MMP-mediated.

Leptin protects rat articular chondrocytes from cytotoxicity induced by TNF-α in the presence of cyclohexamide S.W. Lee, J.H. Rho, S.Y. Lee, J.H. Kim,

Micromechanical mapping of early osteoarthritic changes in the pericellular matrix of human articular cartilage R.E. Wilusz, S. Zauscher, F. Guilak

Intra-articular injection of microRNA-140 (miRNA-140) alleviates osteoarthritis (OA) progression by modulating extracellular matrix (ECM) homeostasis.

Estrogen via estrogen receptor beta partially inhibits mandibular condylar cartilage growth J. Chen, Y. Kamiya, I. Polur, M. Xu, T. Choi, Z. Kalajzic,

Oral and topical boswellic acid attenuates mouse osteoarthritis

NF-κBp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1β-induced and TNF-α-induced chondrocytes Dr C. Lianxu, Ph.D.,

Relative contribution of matrix metalloprotease and cysteine protease activities to cytokine-stimulated articular cartilage degradation B.C. Sondergaard,

J.E. Lafont, F.-A. Poujade, M. Pasdeloup, P. Neyret, F. Mallein-Gerin

Reduced response of human meniscal cells to Osteogenic Protein 1 during osteoarthritis and pro-inflammatory stimulation K.S. Vanderman, R.F. Loeser,

Anti-apoptotic effect of transforming growth factor-β1 on human articular chondrocytes: role of protein phosphatase 2A M. Lires-Deán, B.S., B. Caramés,

Establishment of a rabbit model to study the influence of advanced glycation end products accumulation on osteoarthritis and the protective effect of.

Interaction between zonal populations of articular chondrocytes suppresses chondrocyte mineralization and this process is mediated by PTHrP J. Jiang,

K. Murata, N. Kanemura, T. Kokubun, T. Fujino, Y. Morishita, K

MicroRNA-320 regulates matrix metalloproteinase-13 expression in chondrogenesis and interleukin-1β-induced chondrocyte responses F. Meng, Z. Zhang, W.

A.J. McGregor, B.G. Amsden, S.D. Waldman Osteoarthritis and Cartilage

Pro-inflammatory stimulation of meniscus cells increases production of matrix metalloproteinases and additional catabolic factors involved in osteoarthritis.

Exaggerated inflammatory environment decreases BMP-2/ACS-induced ectopic bone mass in a rat model: implications for clinical use of BMP-2 R.-L. Huang,

Α-MSH inhibits TNF-α-induced matrix metalloproteinase-13 expression by modulating p38 kinase and nuclear factor κB signaling in human chondrosarcoma HTB-94.

Toward scaffold-based meniscus repair: effect of human serum, hyaluronic acid and TGF-ß3 on cell recruitment and re-differentiation U. Freymann, M. Endres,

TGF-β isoforms in renal fibrogenesis

Different doses of strontium ranelate and mechanical vibration modulate distinct responses in the articular cartilage of ovariectomized rats A.G.H. Mierzwa,

The use of hyperosmotic saline for chondroprotection: implications for orthopaedic surgery and cartilage repair N.M. Eltawil, S.E.M. Howie, A.H.R.W.

K. Zou, M.D., G. Liu, M.D., T. Wu, M.D., L. Du, M.D.

Interleukin-1β and interleukin-6 disturb the antioxidant enzyme system in bovine chondrocytes: a possible explanation for oxidative stress generation

Activation of Adenylyl Cyclase Reduces TGF-β Profibrotic Response in Osteoarthritic Fibroblast-Like Synoviocytes M. Qadri, R. Ostrom, K.A. Elsaid Osteoarthritis.

Oral salmon calcitonin reduces cartilage and bone pathology in an osteoarthritis rat model with increased subchondral bone turnover R.H. Nielsen, A.-C.

Oxidative stress induces expression of osteoarthritis markers procollagen IIA and 3B3(−) in adult bovine articular cartilage I.M. Khan, Ph.D., S.J. Gilbert,

Protective effects of a cathepsin K inhibitor, SB , in the canine partial medial meniscectomy model of osteoarthritis J.R. Connor, C. LePage, B.A.

Calcitonin directly attenuates collagen type II degradation by inhibition of matrix metalloproteinase expression and activity in articular chondrocytes

Characterization of pro-apoptotic and matrix-degradative gene expression following induction of osteoarthritis in mature and aged rabbits Dr. C.M. Robertson,

Joint distraction attenuates osteoarthritis by reducing secondary inflammation, cartilage degeneration and subchondral bone aberrant change Y. Chen,

Deletion of 12/15-lipoxygenase accelerates the development of aging-associated and instability-induced osteoarthritis L. Habouri, F.E. El Mansouri, Y.

Lentiviral vector-mediated shRNAs targeting a functional isoform of the leptin receptor (Ob-Rb) inhibit cartilage degeneration in a rat model of osteoarthritis

Volume 23, Issue 8, Pages (August 2015)

Effects of a metalloproteinase inhibitor on osteochondral angiogenesis, chondropathy and pain behavior in a rat model of osteoarthritis P.I. Mapp, D.A.

TGF-β is a potent inducer of Nerve Growth Factor in articular cartilage via the ALK5- Smad2/3 pathway. Potential role in OA related pain? E.N. Blaney.

T. Kurth, M. Sc. , E. Hedbom, Ph. D. , N. Shintani, Ph. D. , M

Pharmaceutical nanocarrier association with chondrocytes and cartilage explants: influence of surface modification and extracellular matrix depletion

S.D. Waldman, J. Usprech, L.E. Flynn, A.A. Khan

Loss of Frzb and Sfrp1 differentially affects joint homeostasis in instability-induced osteoarthritis S. Thysen, F.P. Luyten, R.J. Lories Osteoarthritis.

Joint instability leads to long-term alterations to knee synovium and osteoarthritis in a rabbit model C. Egloff, D.A. Hart, C. Hewitt, P. Vavken, V.

Osteoarthritis and Cartilage

Regulation of mechanical stress-induced MMP-13 and ADAMTS-5 expression by RUNX-2 transcriptional factor in SW1353 chondrocyte-like cells T. Tetsunaga,

Activation of Indian hedgehog promotes chondrocyte hypertrophy and upregulation of MMP-13 in human osteoarthritic cartilage F. Wei, J. Zhou, X. Wei,

Preliminary results of a phase II randomized study to determine the efficacy and safety of genetically engineered allogeneic human chondrocytes expressing.

Mevastatin reduces cartilage degradation in rabbit experimental osteoarthritis through inhibition of synovial inflammation Y. Akasaki, M.D., S. Matsuda,

Who should have a joint replacement? A plea for more ‘phronesis’

J.L. Huebner, J.M. Williams, M. Deberg, Y. Henrotin, V.B. Kraus

Synergistic effect of chondroitin sulfate and cyclic pressure on biochemical and morphological properties of chondrocytes from articular cartilage G.

Chondroitin sulfate modulation of matrix and inflammatory gene expression in IL-1β- stimulated chondrocytes – study in hypoxic alginate bead cultures

Pre-emptive, early, and delayed alendronate treatment in a rat model of knee osteoarthritis: effect on subchondral trabecular bone microarchitecture and.

D.J. Hunter Osteoarthritis and Cartilage

Chondrogenic progenitor cells promote vascular endothelial growth factor expression through stromal-derived factor-1 S. Wang, C. Zhou, H. Zheng, Z. Zhang,

Knee cartilage defects: association with early radiographic osteoarthritis, decreased cartilage volume, increased joint surface area and type II collagen.

Parathyroid hormone (1-34) prevents cartilage degradation and preserves subchondral bone micro-architecture in guinea pigs with spontaneous osteoarthritis

Effects of helium–neon laser on the mucopolysaccharide induction in experimental osteoarthritic cartilage Y.-S. Lin, M.Sc, Dr M.-H. Huang, M.D., Ph.D.,

Osteoarthritis year in review 2016: mechanics

The chondrocyte primary cilium

Chondro-protective effects of low intensity pulsed ultrasound

IGF-1 regulation of type II collagen and MMP-13 expression in rat endplate chondrocytes via distinct signaling pathways M. Zhang, Ph.D., Q. Zhou, M.D.,

Presentation transcript:

Vitamin D prevents articular cartilage erosion by regulating collagen II turnover through TGF-β1 in ovariectomized rats S. Li, G. Niu, Y. Wu, G. Du, C. Huang, X. Yin, Z. Liu, C. Song, H. Leng Osteoarthritis and Cartilage Volume 24, Issue 2, Pages 345-353 (February 2016) DOI: 10.1016/j.joca.2015.08.013 Copyright © 2015 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 (A) Rat body weights and (B) uterus weights for all the groups. Values are the means ± 95% CI. Osteoarthritis and Cartilage 2016 24, 345-353DOI: (10.1016/j.joca.2015.08.013) Copyright © 2015 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Changes in the serum 1α,25(OH)2D3 (A and B), 17β-estradiol (C and D), and serum C-telopeptide of type II collagen (CTX-II) (E and F) levels in all the groups (SHAM+CTL [●], OVX+CTL [■], SHAM+VDD [○], OVX+VDD [□], OVX+LOW [▲], OVX+MID [▽] and OVX+HIGH [△]). Values are the means ± 95% CI. P values are listed in Table I. Osteoarthritis and Cartilage 2016 24, 345-353DOI: (10.1016/j.joca.2015.08.013) Copyright © 2015 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Sections from part of the knees of different groups. OVX+CTL, SHAM+VDD and OVX+VDD showed a similar mode of cartilage surface erosion. Arrows point to cartilage surface erosion. The images in the red frame are higher resolution images of the arrowed area. The histogram shows the quantification results of cartilage surface erosion for the whole joint in all groups. Bars represent 100 μm. Values are the means ± 95% CI. Osteoarthritis and Cartilage 2016 24, 345-353DOI: (10.1016/j.joca.2015.08.013) Copyright © 2015 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Representative expression of transforming growth factor-β1 (TGF-β1), type II collagen (CII), matrix metalloproteinases (MMP)-9,-13 in the articular cartilage of the rats in the following groups: SHAM+CTL, OVX+CTL, SHAM+VDD, OVX+VDD and OVX+HIGH. Representative expression in the OVX+LOW and OVX+MID groups is not shown. IOD means integrated optical density. Bars represent 100 μm. Values are the means ± 95% CI. Osteoarthritis and Cartilage 2016 24, 345-353DOI: (10.1016/j.joca.2015.08.013) Copyright © 2015 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 (A) The concentrations of TGF-β1 in chondrocytes treated with different concentrations of 1α,25(OH)2D3 (0,1–100 nM), stimulated with tumor necrosis factor TNF-α (+) or without TNF-α (−). After TNF-α stimulation, 1α,25(OH)2D3 increased the chondrocyte TGF-β1 expression in a dose-dependent manner. (B) The concentrations of CTX-II in chondrocytes treated with different concentrations of 1α,25(OH)2D3 (0,1–100 nM), stimulated with TNF-α (+) or without TNF-α (−). After TNF-α stimulation, 1α,25(OH)2D3 decreased the chondrocyte CTX-II release in a dose-dependent manner. (C) The CTX-II release from the ECM of chondrocytes treated with/without 10 nM 1α,25(OH)2D3, TNF-α and TGF-β receptor inhibitor (SB505124). The effects of 1α,25(OH)2D3 on TNF-α stimulated chondrocytes were contracted by TGF-β receptor inhibitor. Values are the means ± 95% CI. Osteoarthritis and Cartilage 2016 24, 345-353DOI: (10.1016/j.joca.2015.08.013) Copyright © 2015 Osteoarthritis Research Society International Terms and Conditions