Neurofibromatosis Type 1 Protein and Amyloid Precursor Protein Interact in Normal Human Melanocytes and Colocalize with Melanosomes Sofie De Schepper,

Slides:

Advertisements

Similar presentations

Crucial Roles of MZF1 and Sp1 in the Transcriptional Regulation of the Peptidylarginine Deiminase Type I Gene (PADI1) in Human Keratinocytes Sijun Dong,

Advertisements

Regulation of Human Epidermal Melanocyte Biology By β-Endorphin

Staphylococcus aureus Stimulates Neutrophil Targeting Chemokine Expression in Keratinocytes through an Autocrine IL-1α Signaling Loop Florina Olaru,

Identification of miR-145 as a Key Regulator of the Pigmentary Process

Transient Bullous Dermolysis of the Newborn Associated with Compound Heterozygosity for Recessive and Dominant COL7A1 Mutations Nadja Hammami-Hauasli,

The Autoantigen in Anti-p200 Pemphigoid Is Synthesized by Keratinocytes and Fibroblasts and Is Distinct from Nidogen-2 Silke C. Hofmann, Ursula Voith,

Zhuo Li, Dieter Metze, Dorothea Nashan, Carsten Müller-Tidow, Hubert L

Role of aPKC Isoforms and Their Binding Partners Par3 and Par6 in Epidermal Barrier Formation Iris Helfrich, Annika Schmitz, Paola Zigrino, Christian.

Knockdown of Myosin Va Isoforms by RNAi as a Tool to Block Melanosome Transport in Primary Human Melanocytes Mireille Van Gele, Barbara Geusens, Anne-Marie.

Protease-Activated Receptor-2 (PAR-2) Expression in Human Fibroblasts is Regulated by Growth Factors and Extracellular Matrix Barry L. Gruber, Mary J.

Pro-opiomelanocortin-Related Peptides, Prohormone Convertases 1 and 2 and the Regulatory Peptide 7B2 are Present in Melanosomes of Human Melanocytes

Elisabeth Riedl, Yayoi Tada, Mark C. Udey

Localization of Calcineurin/NFAT in Human Skin and Psoriasis and Inhibition of Calcineurin/NFAT Activation in Human Keratinocytes by Cyclosporin A Wael.

Kinesin and Kinectin Can Associate with the Melanosomal Surface and Form a Link with Microtubules in Normal Human Melanocytes1 Garnet Vancoillie, Jo.

Fusako Sato, Gyula Soos, Charles Link, Kenzo Sato

Colocalization of Kindlin-1, Kindlin-2, and Migfilin at Keratinocyte Focal Adhesion and Relevance to the Pathophysiology of Kindler Syndrome J.E. Lai-Cheong,

Marie-Thérèse Leccia Journal of Investigative Dermatology

Analysis of Proteins with Caseinolytic Activity in a Human Stratum Corneum Extract Revealed a Yet Unidentified Cysteine Protease and Identified the So-Called.

Mitsutoshi Tominaga, Hideoki Ogawa, Kenji Takamori

Novel Homozygous and Compound Heterozygous COL17A1 Mutations Associated with Junctional Epidermolysis Bullosa Michaela Floeth, Heike Schäcke, Nadja Hammami-Hauasli,

Naohito Hatta, Craig Dixon, Amanda J. Ray, Sion R. Phillips, William J

The 130-kDa Glycoform of CD43 Functions as an E-Selectin Ligand for Activated Th1 Cells In Vitro and in Delayed-Type Hypersensitivity Reactions In Vivo

Significance of the S100A4 Protein in Psoriasis

Min Qin, Aslan Pirouz, Myung-Hwa Kim, Stephan R. Krutzik, Hermes J

Identification and Sequencing of a Putative Variant of Proopiomelanocortin in Human Epidermis and Epidermal Cells in Culture Gong Can, Zalfa Abdel-Malek,

The Vesicular Acetylcholine Transporter Is Present in Melanocytes and Keratinocytes in the Human Epidermis Souna M.A. Elwary, Bhaven Chavan, Karin U.

Sri Rajalakshmi Rudrabhatla, Christie L. Mahaffey, Mark E. Mummert

The Role of the Calcium Sensing Receptor in Regulating Intracellular Calcium Handling in Human Epidermal Keratinocytes Chia-Ling Tu, Wenhan Chang, Daniel.

H. Randolph Byers, Mina Yaar, Mark S. Eller, Nicole L

Histidine Decarboxylase Expression in Human Melanoma

Cloning and Characterization of the Expression Pattern of a Novel Splice Product MIA (Splice) of Malignant Melanoma-derived Growth-inhibiting Activity.

Olga M. Mazina, Marjorie A. Phillips, Trevor Williams, Carol A

Min Qin, Aslan Pirouz, Myung-Hwa Kim, Stephan R. Krutzik, Hermes J

Inherited Junctional Epidermolysis Bullosa in the German Pointer: Establishment of a Large Animal Model Annabelle Capt, Flavia Spirito, Eric Guaguere,

Noritaka Oyama, Keiji Iwatsuki, Yoshimi Homma, Fumio Kaneko

Naoko Kanda, Shinichi Watanabe Journal of Investigative Dermatology

Interleukin-13 Receptor in Psoriatic Keratinocytes: Overexpression of the mRNA and Underexpression of the Protein Juan C. Cancino-Díaz, Elba Reyes-Maldonado,

Expression of T-Cadherin in Basal Keratinocytes of Skin

Interactions of Human Myosin Va Isoforms, Endogenously Expressed in Human Melanocytes, Are Tightly Regulated by the Tail Domain Wendy Westbroek, Jo Lambert,

Molecular Architecture of Tight Junctions of Periderm Differs From That of the Maculae Occludentes of Epidermis Kazumasa Morita, Yoko Yoshida, Yoshiki.

Masahiro Hara, Mina Yaar, H

Amanda Helip-Wooley, Wendy Westbroek, Heidi M

Regulation of the Expression of Peptidylarginine Deiminase Type II Gene (PADI2) in Human Keratinocytes Involves Sp1 and Sp3 Transcription Factors Sijun.

Role of Cytoplasmic Dynein in Perinuclear Aggregation of Phagocytosed Melanosomes and Supranuclear Melanin Cap Formation in Human Keratinocytes H. Randolph.

Rab3a and SNARE Proteins: Potential Regulators of Melanosome Movement

Decreased Prostaglandin E2 Production by Inflammatory Cytokine and Lower Expression of EP2 Receptor Result in Increased Collagen Synthesis in Keloid.

Erythema Multiforme Associated Human Autoantibodies Against Desmoplakin I and II: Biochemical Characterization and Passive Transfer Studies Into Newborn.

Multiple Epidermal Connexins are Expressed in Different Keratinocyte Subpopulations Including Connexin 31 Wei-Li Di, Elizabeth L. Rugg, Irene M. Leigh,

The Effect of Thioredoxin on the Expression of Proopiomelanocortin-Derived Peptides, the Melanocortin 1 Receptor and Cell Survival of Normal Human Keratinocytes

Compound Heterozygosity for Novel Splice Site Mutations in the BPAG2/COL17A1 Gene Underlies Generalized Atrophic Benign Epidermolysis Bullosa Leena Pulkkinen,

A Novel Gene Expressed in Human Keratinocytes with Long-Term In Vitro Growth Potential is Required for Cell Growth Laure Aurelian, Cynthia C. Smith,

Regine Keller-Melchior, Rodney Schmidt, Michael Piepkorn

ΔNp63α Promotes Apoptosis of Human Epidermal Keratinocytes

Expression of FcRn, the MHC Class I-Related Receptor for IgG, in Human Keratinocytes Karla Cauza, Gabriele Hinterhuber, Ruth Dingelmaier-Hovorka, Karin.

M2 Muscarinic Acetylcholine Receptor (mAchR) Subtype Is Present in Human Epidermal Keratinocytes In Situ and In Vitro S.M.A. Elwary, S. Hasse, K.U. Schallreuter

Shigeru Kusuda, Cui Chang-Yi, Masae Takahashi, Tadashi Tezuka

Identification of Transglutaminase 3 Splicing Isoforms

Expression of Opsin Molecule in Cultured Murine Melanocyte

A Lack of Birbeck Granules in Langerhans Cells Is Associated with a Naturally Occurring Point Mutation in the Human Langerin Gene Pauline Verdijk, Remco.

Autoantibodies to BP180 Associated with Bullous Pemphigoid Release Interleukin-6 and Interleukin-8 from Cultured Human Keratinocytes Enno Schmidt, Stanislaus.

Requirement of Dynactin p150Glued Subunit for the Functional Integrity of the Keratinocyte Microparasol H. Randolph Byers, Sarah G. Dykstra, Sandrine.

Myosin V Colocalizes with Melanosomes and Subcortical Actin Bundles Not Associated with Stress Fibers in Human Epidermal Melanocytes Jo Lambert, Yves.

The Human Dermokine Gene: Description of Novel Isoforms with Different Tissue- Specific Expression and Subcellular Location Eve Toulza, Marie-Florence.

The Modulation of Aromatase and Estrogen Receptor Alpha in Cultured Human Dermal Papilla Cells by Dexamethasone: A Novel Mechanism for Selective Action.

Naoko Kanda, Shinichi Watanabe Journal of Investigative Dermatology

Permeability Barrier Disruption Increases the Level of Serine Palmitoyltransferase in Human Epidermis Francesca Alessandrini, Dr., Heidrun Behrendt

LEDGF/DFS70, a Major Autoantigen of Atopic Dermatitis, Is a Component of Keratohyalin Granules Kazumitsu Sugiura, Yoshinao Muro, Yuji Nishizawa, Miyako.

Redistribution of LRIG Proteins in Psoriasis

Bcl-2 and bcl-xL Antisense Oligonucleotides Induce Apoptosis in Melanoma Cells of Different Clinical Stages Robert A. Olie, Christoph Hafner, Renzo Küttel,

Matrix Metalloproteinase Inhibitor BB-3103 Unlike the Serine Proteinase Inhibitor Aprotinin Abrogates Epidermal Healing of Human Skin Wounds Ex Vivo1

Presentation transcript:

Neurofibromatosis Type 1 Protein and Amyloid Precursor Protein Interact in Normal Human Melanocytes and Colocalize with Melanosomes Sofie De Schepper, Joachim M.A. Boucneau, Wendy Westbroek, Mieke Mommaas, Jos Onderwater, Ludwine Messiaen, Jean-Marie A.D. Naeyaert, Jo L.W. Lambert Journal of Investigative Dermatology Volume 126, Issue 3, Pages 653-659 (March 2006) DOI: 10.1038/sj.jid.5700087 Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Identification of neurofibromin interacting proteins. (a) Neurofibromin in cell lysate of primary human epidermal melanocytes was immunoprecipitated with NF1 sc-67 antibody and the immunoprecipitate was analyzed by SDS-PAGE (6%) and silver stained. Lane C represents the rabbit polyclonal IgG isotype matched control, lane IP represents the neurofibromin immunoprecipitate. Molecular weight markers are shown on the left and on the right black arrows indicate the position of immunoprecipitated protein bands (p70, p110, p130, p150, p190). (b) Upper right figure: immunostaining with APP (CT15) antibody of NF1 sc-67 immunoprecipitate (left lane) compared to the total cell lysate (right lane). Lower right figure: immunoblotting with NF1 sc-67 antibody of APP (CT15) immunoprecipitate (left lane) compared to the total cell lysate (right lane). Molecular weight markers are shown in kDa. Journal of Investigative Dermatology 2006 126, 653-659DOI: (10.1038/sj.jid.5700087) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Expression of NF1 gene (product) and APP gene (product) in primary human epidermal melanocytes. (a) Detection of NF1 (left) and APP (right) mRNA expression by RT-PCR analysis in primary human epidermal melanocytes (obtained from two different donors (1 and 2)), displaying the expression of both the 751 and 770 isoforms of APP. The same samples showed the appropriate 94bp band (arrow) when analyzed with the exon 36–37 NF1 boundary primers. (b) Expression of neurofibromin and APP proteins was analyzed by SDS-PAGE (6%) followed by immunostaining with anti-neurofibromin (NF1 sc-67) and anti-APP (CT15) antibodies. Molecular weight markers are shown in kDa on the left. Note the immature and mature isoforms of APP. Journal of Investigative Dermatology 2006 126, 653-659DOI: (10.1038/sj.jid.5700087) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Subcellular distribution and localization of neurofibromin, APP and melanosomes in healthy donor (NF1+/+) and NF1 patient (NF1+/−) primary human epidermal melanocytes. Healthy donor melanocytes were cultured on coverslips for 24hours, fixed and immunostained with (a) NKI-beteb and neurofibromin and (b) NKI-beteb and APP. The overlay panels show remarkable colocalization of neurofibromin especially in the perinuclear area and of APP in the perinuclear area and the dendrite tips. NF1 patient melanocytes were cultured on coverslips for 24hours, fixed and immunostained with (c) NKI-beteb and neurofibromin and (d) NKI-beteb and APP. A clear absence of colocalization can be seen. Bars=10μm. Journal of Investigative Dermatology 2006 126, 653-659DOI: (10.1038/sj.jid.5700087) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Ultrastructural distribution and localization of neurofibromin, APP and melanosomes in healthy donor (NF1+/+) and NF1 patient (NF1+/−) primary human epidermal melanocytes. Paraformaldehyde-fixed ultrathin cryosections of healthy donor primary human epidermal melanocytes were labeled with (a) β-amyloid antibody (15nm gold) and (b) neurofibromin antibody (15nm gold). Both APP and neurofibromin showed melanosomal localization. (c) A double labeling with NKI-beteb antibody (15nm gold) and β-amyloid antibody (10nm gold) revealed complexes associated on melanosomes (asterisk). The plasmamembrane of the dendrite is marked with arrows. (d) A double labeling with NKI-beteb antibody (15nm gold) and anti-neurofibromin (10nm gold) showed several melanosome-associated colocalization complexes (plus signs). Paraformaldehyde-fixed ultrathin cryosections of NF1 patient primary human epidermal melanocytes were labeled with (e) APP antibody (15nm gold) and (f) neurofibromin antibody (15nm gold). No melanosomal localization was seen. (g) The double labeling with NKI-beteb (10nm gold) and APP (15nm gold) and with (h) NKI-beteb (10nm gold) and neurofibromin (15nm gold) only showed clear labeling of the melanosomal marker NKI-beteb but no colocalization of (g) APP or (h) neurofibromin. Bars=500nm. Journal of Investigative Dermatology 2006 126, 653-659DOI: (10.1038/sj.jid.5700087) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions