Replacement of sodium with choline in slow-cooling media improves human ovarian tissue cryopreservation Riccardo Talevi, Vincenza Barbato, Valentina.

Slides:

Advertisements

Similar presentations

Development of guideline-based quality indicators for recurrent miscarriage E. van den Boogaard, M. Goddijn, N.J. Leschot, F. van der Veen, J.A.M. Kremer,

Advertisements

Effect of androgen supplementation or modulation on ovarian stimulation outcome in poor responders: a meta-analysis Sesh Kamal Sunkara, Jyotsna Pundir,

Programmes and prospects for ovotechnology

Cancer and fertility: strategies to preserve fertility

Kay Neumann, Georg Griesinger Reproductive BioMedicine Online

Vuong Thi Ngoc Lan, Nguyen Khanh Linh, Ho Manh Tuong, P. C

Diet and endometriosis risk: A literature review

Failure mode and effects analysis of witnessing protocols for ensuring traceability during IVF Laura Rienzi, Fiorenza Bariani, Michela Dalla Zorza, Stefania.

Fresh and cryopreserved ovary transplantation and resting follicle recruitment Sherman Silber, Jorge Pineda, Kathleen Lenahan, Michael DeRosa, Jeffrey.

Low-dosage clomiphene reduces premature ovulation rates and increases transfer rates in natural-cycle IVF M von Wolff, M Nitzschke, P Stute, N Bitterlich,

Protective effect of resveratrol against oxidative damage to ovarian reserve in female Sprague–Dawley rats Pınar Özcan, Cem Fıçıcıoğlu, Özge Kızılkale.

L. L. Sun, Z. Y. Sun, P. Zhang, X. W. Zhai, J. Tang, Q. J. Pan, Q. H

Improving ovarian tissue cryopreservation for oncologic patients: slow freezing versus vitrification, effect of different procedures and devices Sonia.

Confocal laser scanning microscopy analysis of bioenergetic potential and oxidative stress in fresh and frozen-thawed human ovarian tissue from oncologic.

Successful slush nitrogen vitrification of human ovarian tissue

Claus Yding Andersen, Stine Gry Kristensen

In vitro maturation improves oocyte or embryo cryopreservation outcome in breast cancer patients undergoing ovarian stimulation for fertility preservation

Association between blastocyst morphology and outcome of single-blastocyst transfer Etienne Van den Abbeel, Basak Balaban, Søren Ziebe, Kersti Lundin,

Failure mode and effects analysis of witnessing protocols for ensuring traceability during IVF Laura Rienzi, Fiorenza Bariani, Michela Dalla Zorza, Stefania.

Effects of HMG on revascularization and follicular survival in heterotopic autotransplants of mouse ovarian tissue Yanrong Wang, Qing Chang, Jing Sun,

Silke Klocke, Claudia Tappehorn, Georg Griesinger

N-acetylcysteine improves function and follicular survival in mice ovarian grafts through inhibition of oxidative stress Monireh Mahmoodi, Malek Soleimani.

Sherman Silber Reproductive BioMedicine Online

Why are reproductive cancers more common in nulliparous women?

M.M. Francou, J. Ten, R. Bernabeu, J. De Juan

Apoptosis in normal ovaries of women with and without endometriosis

Effect of N-acetyl-cysteine after ovarian drilling in clomiphene citrate-resistant PCOS women: a pilot study A. Nasr Reproductive BioMedicine Online

Claus Yding Andersen, Sherman J

A. Arav, Z. Gavish, A. Elami, Y. Natan, A. Revel, S. Silber, R. G

Optimization of protocols for human ovarian tissue cryopreservation with sucrose, 1,2- propanediol and human serum Raffaella Fabbri, Gianandrea Pasquinelli,

Survival and growth of human preantral follicles after cryopreservation of ovarian tissue, follicle isolation and short-term xenografting Fernanda Paulini,

Lisa Cummins, Juliette Koch, Suha Kilani

The incidence of long heterochromatic polymorphism variants in infants conceived through assisted reproductive technologies Andrew Wilson, Katherine.

Follicle features in adolescent and young adult women with Hodgkin’s disease prior to chemotherapy: a preliminary report Raffaella Fabbri, Gianandrea.

N-acetylcysteine leads to greater ovarian protection than enoxaparin sodium in a rat ovarian torsion model Gulcin Sahin Ersoy, Meryem Eken, Reshef Tal,

Ronit Machtinger, Raoul Orvieto Reproductive BioMedicine Online

Preservation of human ovarian follicles within tissue frozen by vitrification in a xeno-free closed system using only ethylene glycol as a permeating.

Donor information considered important to donors, recipients and offspring: an Australian perspective I.S. Rodino, P.J. Burton, K.A. Sanders Reproductive.

Reprint of: Theoretical and experimental basis of slow freezing

Impact of vitamin D levels on ovarian reserve and ovarian response to ovarian stimulation in oocyte donors Alberta Maria Fabris, Maria Cruz, Carlos Iglesias,

V Isachenko, E Isachenko, R Kreienberg, M Woriedh, JM Weiss

Platelet-rich plasma promotes the development of isolated human primordial and primary follicles to the preantral stage Laleh Hosseini, Abolfazl Shirazi,

Stanley Paul Leibo (1937–2014): scientist, mentor and friend

Follicle recruitment determines IVF productivity rate via the number of embryos frozen and subsequent transfers James D. Stanger, John L. Yovich Reproductive.

Should we be promoting embryo transfer at blastocyst stage?

Gulcin Sahin Ersoy, Meryem Kurek Eken, Ozge Cevik, Ozlem T

Stephen C. Collins, Esther Chan Reproductive BioMedicine Online

Endometrial nerve fibre density in patients undergoing IVF: a pilot study Suzanna Wand, Ariel Weissman, Ron Sagiv, Letizia Schreiber, Mona Boaz, Eran.

W.H. Wallace, T.W. Kelsey, R.A. Anderson

Pietro Gambadauro, Ramesan Navaratnarajah

Implication of apoptosis in sperm cryoinjury

Genome-wide analysis shows no genomic predictors of ovarian response to stimulation by exogenous FSH for IVF Jeroen van Disseldorp, Lude Franke, Rene.

The Alpha Consensus Meeting on the professional status of the clinical embryologist: proceedings of an expert meeting Reproductive BioMedicine Online

Response: time-lapse systems for ART

C Almeida, M Sousa, A Barros Reproductive BioMedicine Online

Outcomes of immature oocytes collected from ovarian tissue for cryopreservation in adult and prepubertal patients Giovanna Fasano, Julie Dechène, Raffaella.

Propofol versus thiopental sodium as anaesthetic agents for oocyte retrieval: a randomized controlled trial Evangelia Goutziomitrou, Christos A. Venetis,

Nigel Pereira, Anna Voskuilen-Gonzalez, Kolbe Hancock, Jovana P

Glucose/lactate metabolism of cryopreserved intact bovine ovaries as a novel quantitative marker to assess tissue cryodamage R. Gerritse, C.C.M. Beerendonk,

Theoretical and experimental basis of slow freezing

Ovarian reserve screening before contraception?

Cryobanking of human ovarian tissue: do women still want their tissue stored beyond 5 years? Kirsten Tryde Macklon, Erik Ernst, Anders Nyboe Andersen,

Implantation of fresh and thawed–warmed embryos in single embryo transfer cycles: interpreting the initial beta-HCG Cynthia K. Sites, Peter St Marie,

Programmes and prospects for ovotechnology

Claudio Manna, Loris Nanni, Alessandra Lumini, Sebastiana Pappalardo

Yakoub Khalaf, Susan Bewley, Peter Braude

M. Franz, J. Ott, A. Watrelot, L. Küssel, H. Husslein

Fertility in cancer patients after cryopreservation of one ovary

A. Arav, Z. Gavish, A. Elami, Y. Natan, A. Revel, S. Silber, R. G

Use of progestins to inhibit spontaneous ovulation during ovarian stimulation: the beginning of a new era? Antonio La Marca, Martina Capuzzo Reproductive.

Presentation transcript:

Replacement of sodium with choline in slow-cooling media improves human ovarian tissue cryopreservation Riccardo Talevi, Vincenza Barbato, Valentina Mollo, Ilaria Fiorentino, Cristoforo De Stefano, Fabio Maria Guarino, Roberto Gualtieri Reproductive BioMedicine Online Volume 27, Issue 4, Pages 381-389 (October 2013) DOI: 10.1016/j.rbmo.2013.07.003 Copyright © 2013 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Human primordial/primary follicle grading of grade-1 (A, D, G, J), grade-2 (B, E, H, K) and grade-3 (C, F, I, L) human ovarian follicles. (A–C) Histology, haematoxylin/eosin staining. (D–L) Immunohistochemical localization of apoptotic markers: p53 (D–F), p21 (G–I) and Apaf-1 (J–L) immunohistochemical labelling. Bar=10μm. Reproductive BioMedicine Online 2013 27, 381-389DOI: (10.1016/j.rbmo.2013.07.003) Copyright © 2013 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Distribution of grade-1–3 follicles in fresh and cryopreserved ovarian tissue. C=fresh control; P-Na=PrOH-Na-based slow cooling; P-Ch=PrOH-choline-based slow cooling; D-Na=DMSO-Na-based slow cooling; D-Ch=DMSO-choline-based slow cooling. ∗P<0.05 versus fresh control; ∗∗P<0.01 versus fresh control. Reproductive BioMedicine Online 2013 27, 381-389DOI: (10.1016/j.rbmo.2013.07.003) Copyright © 2013 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Transmission electron micrographs of fresh (A, B) and cryopreserved (C–J) ovarian tissue slow cooled with PrOH-Na (C, D), PrOH-choline (E, F), DMSO-Na (G, H) and DMSO-choline (I, J). Asterisks indicate swollen mitochondria; arrowheads indicate degenerated mitochondria. Bars=10μm (A, C, E, G), 2μm (B, D, F, H, J), 3μm (I). Reproductive BioMedicine Online 2013 27, 381-389DOI: (10.1016/j.rbmo.2013.07.003) Copyright © 2013 Reproductive Healthcare Ltd. Terms and Conditions

Figure 4 Proportion of damaged mitochondria in oocytes in fresh and cryopreserved ovarian tissue. C=fresh control; P-Na=PrOH-Na-based slow cooling; P-Ch=PrOH-choline-based slow cooling; D-Na=DMSO-Na-based slow cooling; D-Ch=DMSO-choline-based slow cooling. ∗P<0.05 versus fresh control; ∗∗P<0.01 versus fresh control; ##P<0.01 versus same protocol with sodium-based media. Reproductive BioMedicine Online 2013 27, 381-389DOI: (10.1016/j.rbmo.2013.07.003) Copyright © 2013 Reproductive Healthcare Ltd. Terms and Conditions

Figure 5 Transmission electron micrographs of fresh (A, D) and cryopreserved (B, C, E, F) ovarian stroma in samples slow cooled with PrOH-Na (b), PrOH-choline (e), DMSO-Na (c) and DMSO-choline (f). Asterisks indicate intermediate stages of stromal cell necrosis. Bars=10μm (A), 2μm (B–F). Reproductive BioMedicine Online 2013 27, 381-389DOI: (10.1016/j.rbmo.2013.07.003) Copyright © 2013 Reproductive Healthcare Ltd. Terms and Conditions