Volume 123, Issue 1, Pages (July 2002)

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Volume 123, Issue 1, Pages 365-372 (July 2002) Aberrant methylation of CpG islands in intraductal papillary mucinous neoplasms of the pancreas Norihiro Sato, Takashi Ueki, Noriyoshi Fukushima, Christine A. Iacobuzio–Donahue, Ralph H. Hruban, Michael Goggins, Charles J. Yeo, John L. Cameron Gastroenterology Volume 123, Issue 1, Pages 365-372 (July 2002) DOI: 10.1053/gast.2002.34160 Copyright © 2002 American Gastroenterological Association Terms and Conditions

Fig. 1 Methylation profiles of multiple loci in IPMNs. The methylation status of 7 CpG islands in 51 IPMNs is shown. ■, methylated alleles in the neoplasm; □, unmethylated alleles in the neoplasm. Gastroenterology 2002 123, 365-372DOI: (10.1053/gast.2002.34160) Copyright © 2002 American Gastroenterological Association Terms and Conditions

Fig. 2 Methylation-specific PCR analysis of MICP25 and p16 in IPMNs. DNA samples extracted from normal pancreata (NP) or IPMNs (samples 963, 096, 921, and 040) were amplified with primers specific to the unmethylated (U) or methylated (M) templates after modification with sodium bisulfite. Gastroenterology 2002 123, 365-372DOI: (10.1053/gast.2002.34160) Copyright © 2002 American Gastroenterological Association Terms and Conditions

Fig. 3 Reverse-transcription PCR analysis of ppENK in IPMNs. Strong expression is detected in 2 IPMNs (samples 981 and 816) unmethylated at ppENK, whereas 7 IPMNs having methylated ppENK show a loss of (samples 083, 050, 921, 482, 045, and 004) or weak (sample 979) expression of ppENK mRNA. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) serves as an RNA control, and mRNA from a pancreatic cancer cell line (CFPAC1) treated with the demethylating agent 5-aza-2'-deoxycytidine (5Az-dC) serves as a positive control. Gastroenterology 2002 123, 365-372DOI: (10.1053/gast.2002.34160) Copyright © 2002 American Gastroenterological Association Terms and Conditions

Fig. 4 Immunohistochemical labeling of IPMNs with an anti-p16 monoclonal antibody. Positive nuclear labeling for p16 is shown in low-grade IPMNs with unmethylated p16 (A and B), whereas the nuclear staining is lost in high-grade IPMNs with methylated p16 (C and D). Gastroenterology 2002 123, 365-372DOI: (10.1053/gast.2002.34160) Copyright © 2002 American Gastroenterological Association Terms and Conditions