MN-enriched miRNAs are depleted temporally in ALS rodent model and human patient spinal cord. MN-enriched miRNAs are depleted temporally in ALS rodent.

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MN-enriched miRNAs are depleted temporally in ALS rodent model and human patient spinal cord. MN-enriched miRNAs are depleted temporally in ALS rodent model and human patient spinal cord. A, The MN-enriched miRNAs miR-218 and miR-138 are significantly depleted in ALS mouse (SOD1G93A) model spinal cord. This depletion occurs temporally and is maximized at end stage. n = 3–4. B, Pan-neuronal enriched miRNAs are not significantly depleted in ALS mouse model spinal cord, even at end stage. n = 3–4. C, MN-enriched miR-218 is significantly depleted in ALS rat model spinal cord and more so than the pan-neuronal miRNAs miR-382 and miR-672. n = 4–5. D, miR-218 is significantly depleted in human ALS patient autopsy spinal cord compared with age-matched controls. n = 4–10. Values are expressed as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, one-way ANOVA with multiple comparisons (Dunnett's) (A), Student's unpaired, two-tailed t test (B, D), and two-way ANOVA (C). Relative expression is normalized to RNA input and endogenous miRNA control miR-24 (A, B) or U6 snRNA (C, D). Mariah L. Hoye et al. J. Neurosci. 2017;37:5574-5586 ©2017 by Society for Neuroscience