Northern blot and Western blot analysis of syndecan-4 expression in TF-1 and HEL cells. Northern blot and Western blot analysis of syndecan-4 expression.

Slides:



Advertisements
Similar presentations
A study of the coregulation and tissue specificity of XGand MIC2 gene expression in eukaryotic cells by Claude Fouchet, Pierre Gane, Martine Huet, Marc.
Advertisements

Arterioscler Thromb Vasc Biol
MMP-2, MT1-MMP, and TIMP-2 are essential for the invasive capacity of human mesenchymal stem cells: differential regulation by inflammatory cytokines by.
Comparison of Caspase Activation and Subcellular Localization in HL-60 and K562 Cells Undergoing Etoposide-Induced Apoptosis by Luis M. Martins, Peter.
C-myb Transactivates the Human Cyclin A1 Promoter and Induces Cyclin A1 Gene Expression by Carsten Müller, Rong Yang, Gregory Idos, Nicola Tidow, Sven.
Antiangiogenic antithrombin down-regulates the expression of the proangiogenic heparan sulfate proteoglycan, perlecan, in endothelial cells by Weiqing.
Expression of Chemokines in GVHD Target Organs Is Influenced by Conditioning and Genetic Factors and Amplified by GVHR  Markus Y. Mapara, Corinna Leng,
The Role of Transcription Factor PU
by Martha B. Johnson, and Caroline A. Enns
Upregulation of Cortactin Expression During the Maturation of Megakaryocytes by Xi Zhan, Christian C. Haudenschild, Yangson Ni, Elizabeth Smith, and Cai.
by Mineo Iwata, Lynn Graf, Norihiro Awaya, and Beverly Torok-Storb
by Hans G. Drexler, Roderick A. F. MacLeod, and Willy G. Dirks
Lipopolysaccharide Activates Caspase-1 (Interleukin-1–Converting Enzyme) in Cultured Monocytic and Endothelial Cells by Ralf R. Schumann, Claus Belka,
Microvesicles released by PC-3 cells.
Analysis of CDCP1 as a potential prostate cancer biomarker.
HIV-1 protease inhibitors decrease proliferation and induce differentiation of human myelocytic leukemia cells by Takayuki Ikezoe, Eric S. Daar, Jun-ichi.
Cloning, expression, and functional characterization of the von Willebrand factor–cleaving protease (ADAMTS13)‏ by Barbara Plaimauer, Klaus Zimmermann,
P100: A Novel Proliferation-Associated Nuclear Protein Specifically Restricted to Cell Cycle Phases S, G2 , and M by H.J. Heidebrecht, F. Buck, J. Steinmann,
by Douglas D. Ross, Judith E. Karp, Tar T. Chen, and L. Austin Doyle
Volume 117, Issue 5, Pages (November 1999)
Hodgkin/Reed-Sternberg Cells Induce Fibroblasts to Secrete Eotaxin, a Potent Chemoattractant for T Cells and Eosinophils by Franziska Jundt, Ioannis Anagnostopoulos,
Identification of the host interaction partners of HEV proteins in the human liver and fetal brain cDNA libraries. Identification of the host interaction.
Gene expression and immunologic consequence of SPAN-Xb in myeloma and other hematologic malignancies by Zhiqing Wang, Yana Zhang, Haichao Liu, Emanuela.
by Alan E. Mast, Jason E. Stadanlick, J. Marcus Lockett, and Dennis J
Volume 60, Issue 1, Pages (July 2001)
Constitutive STAT6 activation in primary mediastinal large B-cell lymphoma by Chrystelle Guiter, Isabelle Dusanter-Fourt, Christiane Copie-Bergman, Marie-Laure.
by Xingwei Sui, Sanford B. Krantz, Min You, and Zhizhuang Zhao
Expression and regulation of MD-2s.
Lineage-Restricted Expression of Protein Kinase C Isoforms in Hematopoiesis by Alessandra Bassini, Giorgio Zauli, Giovanni Migliaccio, Anna Rita Migliaccio,
HES1 is a novel interactor of the Fanconi anemia core complex
Volume 54, Issue 1, Pages (July 1998)
Human chronic lymphocytic leukemia B cells can escape DNA damage-induced apoptosis through the nonhomologous end-joining DNA repair pathway by Ludovic.
Interferon- Activates Multiple STAT Proteins and Upregulates Proliferation-Associated IL-2R, c-myc, and pim-1 Genes in Human T Cells by Sampsa Matikainen,
by T. G. Willis, I. R. Zalcberg, L. J. A. Coignet, I. Wlodarska, M
A quantitative proteomics strategy to identify SUMO-conjugated proteins. A quantitative proteomics strategy to identify SUMO-conjugated proteins. HeLa.
Volume 118, Issue 6, Pages (June 2000)
Cell-Density-Dependent Regulation of Expression and Glycosylation of Dopachrome Tautomerase/Tyrosinase-Related Protein-2  Thomas J. Hornyak, Daniel J.
Induction of HS proteoglycan expression during early erythroid differentiation in the murine multipotent cell line FDCP-Mix A4. Induction of HS proteoglycan.
A, Western blot analysis of fetuin-A in AGA (lanes 1–5 and 10–13) and IUGR (lanes 6–9, 14, and 15) UC plasma samples. A, Western blot analysis of fetuin-A.
Inhibition of ADAMTS-7 and ADAMTS-12 degradation of cartilage oligomeric matrix protein by alpha-2-macroglobulin  Y. Luan, Ph.D., M.D., L. Kong, Ph.D.,
Western blot analysis of fetuin-A in AGA and IUGR UC plasma after digestion with glycosidases showing the elimination of the IUGR-related isoforms following.
Glucosamine sulfate modulates the levels of aggrecan and matrix metalloproteinase-3 synthesized by cultured human osteoarthritis articular chondrocytes 
Expression of σ in SCCs Expression of σ in SCCs. Shown is a magnified section of a representative 2D PAGE gel run with a lysate from an SCC.
Western blotting analysis of purified cytoplasmic membranes.
Overexpression of BTAK/Aurora-A in ovarian carcinoma.
Neutrophils and the S100A9 protein critically regulate granuloma formation by Yuya Yoshioka, Tatsuaki Mizutani, Satoshi Mizuta, Ayumi Miyamoto, Satoru.
Immunoblot analysis of GpGCS1 expression in strains K41 (mating-type plus) and K34 (mating-type minus). Immunoblot analysis of GpGCS1 expression in strains.
Regulation by Interleukin-10 and Interleukin-4 of Cyclooxygenase-2 Expression in Human Neutrophils by Hiroaki Niiro, Takeshi Otsuka, Kenji Izuhara, Kunihiro.
Targeting strategy and molecular verification of myoglobin disruption.
Expression changes of specific epigenetic-controlled tumor-related genes by the prenatal/maternal BSp treatment. qRT-PCR and Western blot analysis were.
Defective yolk sac vascularization in RapGEF2−/− mice.
FL B cells promote a prolonged CCL2 production by HD-MSCs.
Sequential effects of high glucose on mesangial cell transforming growth factor-β1 and fibronectin synthesis  Jong Hoon Oh, Hunjoo Ha, Mi Ra Yu, Hi Bahl.
Time-dependent effects of IL-6 on tyrosine phosphorylation of IRS-1.
IL-6 inhibits insulin-induced formation of p85/IRS-1 complexes.
IRS-1 and -2 expression in four mesangial cell lines from different D-NOD (A and B) and ND-NOD (C and D) mice. IRS-1 and -2 expression in four mesangial.
Expression of Prostacyclin Receptor in Human Megakaryocytes
CML-HSA treatment activates MAPK family members ERK1/2 and p38 but not JNK. Samples were taken at the indicated times after 100 μg/ml CML-HSA exposure.
Establishment of OL1 cells.
Increased electrophoretic mobility of Cx43 in the ischemic heart involves dephosphorylation at serine residues 325, 328 and/or 330. Increased electrophoretic.
Expression of human tau in GFAP/tau Tg mice.
BME treatment increases granzyme B expression in NK 3.3 cells.
Effects of visfatin on the cell proliferation and phosphorylation of ERK, Akt, and GSK-3β proteins in HCC cells. Effects of visfatin on the cell proliferation.
MiR-200c is a PI3K–AKT signaling pathway regulator in CRC
Curcumin suppresses the expression of antiapoptotic proteins in multiple myeloma cells. Curcumin suppresses the expression of antiapoptotic proteins in.
Effect of SFN on the protein expression of Nrf2, HO-1, and NQO1 in JB6-shMock and JB6-shNrf2 cells. Effect of SFN on the protein expression of Nrf2, HO-1,
Met is expressed in Her2-overexpressing cell lines and Her2 (+) breast tumors. Met is expressed in Her2-overexpressing cell lines and Her2 (+) breast tumors.
Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Expression.
Effect of bevacizumab on the proliferation of A2780 cells.
Effect of SFN on the total activity and protein expression of HDACs in JB6 P+ cells. Effect of SFN on the total activity and protein expression of HDACs.
Presentation transcript:

Northern blot and Western blot analysis of syndecan-4 expression in TF-1 and HEL cells. Northern blot and Western blot analysis of syndecan-4 expression in TF-1 and HEL cells. Northern blot analysis was performed using a digoxigenin-labeled hybridization probe for syndecan-4. A specific signal could be detected for syndecan-4 in TF-1 (A) and HEL (C) cells. GAPDH was used as a control (B and D). For Western blot analysis (E), HS proteoglycans from the supernatant of HEL cells were isolated and digested by heparinase/heparitinase (H) or chondroitinases AC/ABC (A) and submitted to SDS-PAGE on a 4% to 15% gel. The untreated sample is shown on lane N. Detection was performed using the syndecan-4 specific MoAb 8G3. The core protein of syndecan-4 is indicated by an arrowhead. It might be noted that in HEL cells a substantial amount of free core protein of syndecan-4 is detectable. Zofia Drzeniek et al. Blood 1999;93:2884-2897 ©1999 by American Society of Hematology