Hypermethylation of estrogen receptor-α gene in atheromatosis patients and its correlation with homocysteine  Yu-Shan Huang, Yan-Fang Zhi, Shu-Ren Wang  Pathophysiology  Volume 16, Issue 4, Pages 259-265 (October 2009) DOI: 10.1016/j.pathophys.2009.02.010 Copyright © 2009 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Electrophoregram of methylation status with different methylation level in ER-α gene (I) fully methylation sample, (II) half-methylation sample, and (III) non-methylation sample. U: indicated the unmethylated PCR band with primers ER-pU matching the sequences that the cytosine in giving DNA sequences were unmethylated, which would be converted to thymidine after sodium bisulfite treatment. M: indicated the methylated PCR band with primers ER-pM matching the sequences that the cytosine in given DNA sequences were methylated, which would not be influenced by sodium bisulfite treatment, remained to be cytosine. Pathophysiology 2009 16, 259-265DOI: (10.1016/j.pathophys.2009.02.010) Copyright © 2009 Elsevier Ireland Ltd Terms and Conditions

Fig. 2 The alteration of methylation status of ERα gene treated by Hcy with different concentrations and different treating time. (U and M: see Fig. 1 legend). Pathophysiology 2009 16, 259-265DOI: (10.1016/j.pathophys.2009.02.010) Copyright © 2009 Elsevier Ireland Ltd Terms and Conditions

Fig. 3 Semiquantitative RT-PCR assessment of ERα gene expression in HUSMCs treated by Hcy with different treating time **p<0.01 vs control (no drug group). Pathophysiology 2009 16, 259-265DOI: (10.1016/j.pathophys.2009.02.010) Copyright © 2009 Elsevier Ireland Ltd Terms and Conditions

Fig. 4 Effect of Hcy on cell viability: dose-dependent promoting effect of Hcy concentration on SMC proliferation. Pathophysiology 2009 16, 259-265DOI: (10.1016/j.pathophys.2009.02.010) Copyright © 2009 Elsevier Ireland Ltd Terms and Conditions