Xiuqing Wang, Zhongjie Sun, Robert Cade Kidney International

Slides:

Advertisements

Similar presentations

Human tissue kallikrein gene delivery attenuates hypertension, renal injury, and cardiac remodeling in chronic renal failure William C. Wolf, Hideaki.

Advertisements

Volume 67, Issue 2, Pages (February 2005)

Quantitative Detection and Differentiation of Human Herpesvirus 6 Subtypes in Bone Marrow Transplant Patients by Using a Single Real-Time Polymerase Chain.

Volume 65, Issue 4, Pages (April 2004)

Plasma sodium and hypertension

Volume 8, Issue 1, Pages (July 2003)

Volume 69, Issue 6, Pages (March 2006)

Down-regulation of HLA-G boosted natural killer cell-mediated cytolysis in JEG-3 cells cultured in vitro Li Li Sun, M.Sc., Yibing Han, Ph.D., Jian Hui.

Effects of suppressing intrarenal angiotensinogen on renal transforming growth factor- β1 expression in acute ureteral obstruction Gyu-Tae Shin, Wook-Hwan.

Gene Transfer Into the Donor Heart During Cold Preservation for Heart Transplantation Satoshi Gojo, Kazuo Niwaya, Shigeki Taniguchi, Kazuhiko Nishizaki,

Volume 68, Issue 2, Pages (August 2005)

Volume 59, Issue 4, Pages (April 2001)

Volume 60, Issue 6, Pages (December 2001)

Volume 59, Issue 4, Pages (April 2001)

In vivo gene gun–mediated transduction into rat heart with Epstein-Barr virus-based episomal vectors Kazuhiko Nishizaki, MD, Osam Mazda, MD, PhD, Yoshiko.

Adenovirus-mediated kallikrein gene delivery reverses salt-induced renal injury in Dahl salt-sensitive rats Julie Chao, Jenny J. Zhang, Kuei-Fu Lin,

Dieter A. Häberle, Wolfgang Biller, Takuyuki Ise, Christian J. Metz

Rat mesangial α-endosulfine

Volume 58, Issue 4, Pages (October 2000)

Thomas E. Arnold, MD, Dmitri Gnatenko, PhD, Wadie F. Bahou, MD

Transgene Expression in the Brain Stem Effected by Intramuscular Injection of Polyethylenimine/DNA Complexes Shu Wang, Nan Ma, Shujun J. Gao, Hanry Yu,

Comparison of two methods of in vivo gene transfer by electroporation

Volume 79, Issue 5, Pages (March 2011)

Volume 12, Issue 5, Pages (November 2005)

Volume 13, Issue 2, Pages (February 2006)

Suppression of early experimental osteoarthritis by in vivo delivery of the adenoviral vector-mediated NF-κBp65-specific siRNA L.X. Chen, Ph.D., L. Lin,

Volume 64, Issue 2, Pages (August 2003)

Prenatal programming of adult hypertension in the rat

Volume 54, Issue 5, Pages (November 1998)

Gene delivery to in situ veins: Differential effects of adenovirus and adeno-associated viral vectors Mohammad H. Eslami, MD, Sidhu P. Gangadharan, MD,

Volume 76, Issue 3, Pages (August 2009)

PPARα agonist fenofibrate improves diabetic nephropathy in db/db mice

Proinflammatory effects of iron sucrose in chronic kidney disease

Gene transfer of truncated IκBα prevents tubulointerstitial injury

Volume 67, Issue 2, Pages (February 2005)

Renal vascular sclerosis is associated with inherited thrombophilias

Human tissue kallikrein gene delivery attenuates hypertension, renal injury, and cardiac remodeling in chronic renal failure William C. Wolf, Hideaki.

Volume 68, Issue 6, Pages (December 2005)

Julia E. Tank, Orson W. Moe, William L. Henrich Kidney International

Hongli Lin, Xiangmei Chen, Jianzhong Wang, Zhiheng Yu

Volume 57, Issue 1, Pages (January 2000)

Cyclosporine A attenuates the natriuretic action of loop diuretics by inhibition of renal COX-2 expression Klaus Höcherl, Frieder Kees, Bernhard K. Krämer,

Volume 62, Issue 4, Pages (October 2002)

Role of endothelin and nitric oxide imbalance in the pathogenesis of hypoxia-induced arterial hypertension Zhenmin Ni, Shahrooz Bemanian, Salah D. Kivlighn,

Hideyuki Murakami, Katsutoshi Yayama, Lee Chao, Julie Chao

Volume 59, Issue 5, Pages (May 2001)

Volume 59, Issue 4, Pages (April 2001)

Adenovirus-mediated gene transfer to renal glomeruli in rodents

Klotho is a target gene of PPAR-γ

Neurogenic factors and hypertension in renal disease

Volume 56, Issue 5, Pages (November 1999)

Volume 71, Issue 9, Pages (May 2007)

Volume 62, Issue 6, Pages (December 2002)

Aldosterone in the development and progression of renal injury

Volume 75, Issue 12, Pages (June 2009)

Calcium channel blockers, either amlodipine or mibefradil, ameliorate renal injury in experimental diabetes Guorong Ma, Terri J. Allen, Mark E. Cooper,

Human myometrium and leiomyomas express gonadotropin-releasing hormone 2 and gonadotropin-releasing hormone 2 receptor Jason D. Parker, D.O., Minnie.

Volume 71, Issue 9, Pages (May 2007)

Volume 67, Issue 6, Pages (June 2005)

Cultured human glomerular mesangial cells express the C5a receptor

Volume 62, Issue 5, (November 2002)

Increased renal calcium and magnesium transporter abundance in streptozotocin- induced diabetes mellitus C-T. Lee, Y-H.H. Lien, L-W. Lai, J-B. Chen, C-R.

Volume 76, Issue 9, Pages (November 2009)

Volume 58, Issue 6, Pages (December 2000)

Volume 60, Issue 2, Pages (August 2001)

Volume 65, Issue 6, Pages (June 2004)

Volume 65, Issue 6, Pages (June 2004)

Quantitative estimation of renal blood flow by power Doppler ultrasonography in renovascular hypertensive dogs Tetsumasa Miyajima, Hiroshi Yokoyama,

Volume 53, Issue 6, Pages (June 1998)

Fang Wang, Yunfeng Wang, Jie Ding, Jiyun Yang Kidney International

Presentation transcript:

Prolonged attenuation of cold-induced hypertension by adenoviral delivery of renin antisense Xiuqing Wang, Zhongjie Sun, Robert Cade Kidney International Volume 68, Issue 2, Pages 680-687 (August 2005) DOI: 10.1111/j.1523-1755.2005.00446.x Copyright © 2005 International Society of Nephrology Terms and Conditions

Figure 1 Identification of recombinant adenovirus with rat renin antisense (prAdv.RRA) by polymerase chain reaction (PCR). (A) Lanes 1 to 8 show 312bp products, eight plasmids from eight colonies ofEscherichia coli as the templates and Adeno-X™ PCR primers set two as primers. Lane 9 shows 312bp DNA fragment as a positive control. Lane 10 shows 1kb DNA ladder. (B) Lanes 1 to 8 show 747bp products, eight plasmids (the same as the above) as the templates and rat renin-specific primers as primers. Lane 9 shows 1kb DNA ladder. Lane 10 shows 747bp DNA fragment as a positive control. Kidney International 2005 68, 680-687DOI: (10.1111/j.1523-1755.2005.00446.x) Copyright © 2005 International Society of Nephrology Terms and Conditions

Figure 2 Effect of adenoviral delivery of rat renin antisense on cold-induced elevation of blood pressure (mean ± SEM). Intravenous injections of recombinant adenovirus with rat renin antisense (rAdv.RRA) or recombinant adenovirus with LacZ (rAdv.LacZ) were carried out immediately before exposure to cold. Adv.LacZ-Cold are rats treated with rAdv.LacZ and exposed to cold; Adv.RRA-Cold are rats treated with rAdv.RRA and exposed to cold; Adv.RRA-Warm are rats treated with rAdv.RRA and kept at room temperature; Adv.LacZ-Warm are rats treated with rAdv.LacZ and kept at room temperature (N = 6). Kidney International 2005 68, 680-687DOI: (10.1111/j.1523-1755.2005.00446.x) Copyright © 2005 International Society of Nephrology Terms and Conditions

Figure 3 Effect of adenoviral delivery of rat renin antisense on plasma renin activity (PRA) (A), plasma active renin concentration (B), and total plasma renin concentration (C) (mean ± SEM). These were measured when animals were sacrificed at 5 weeks after gene delivery. Adv.LacZ-Cold are rats treated with rAdv.LacZ and exposed to cold; Adv.RRA-Cold are rats treated with rAdv.RRA and exposed to cold; Adv.RRA-Warm are rats treated with rAdv.RRA and kept at room temperature; Adv.LacZ-Warm are rats treated with rAdv.LacZ and kept at room temperature. **P < 0.001, compared with the LacZ-Warm group;++P < 0.001 compared with the LacZ-Cold group (N = 6). Kidney International 2005 68, 680-687DOI: (10.1111/j.1523-1755.2005.00446.x) Copyright © 2005 International Society of Nephrology Terms and Conditions

Figure 4 Effect of adenoviral delivery of rat renin antisense on plasma aldosterone concentration (A) and urinary aldosterone excretion (B) (mean ± SEM). Plasma aldosterone concentration was measured when animals were sacrificed at 5 weeks after gene delivery. Urinary aldosterone excretion was measured during week 3 post-gene delivery. Adv.LacZ-Cold are rats treated with rAdv.LacZ and exposed to cold; Adv.RRA-Cold are rats treated with rAdv.RRA and exposed to cold; Adv.RRA-Warm are rats treated with rAdv.RRA and kept at room temperature; Adv.LacZ-Warm are rats treated with rAdv.LacZ and kept at room temperature. **P < 0.001, compared with the LacZ-Warm group;++P < 0.001 compared with the LacZ-Cold group (N = 6). Kidney International 2005 68, 680-687DOI: (10.1111/j.1523-1755.2005.00446.x) Copyright © 2005 International Society of Nephrology Terms and Conditions

Figure 5 X-gal staining of LacZ in superior mesenteric arteries. X-gal staining was carried out when animals were sacrificed at 5 weeks after gene delivery. Left, X-gal staining of LacZ in a mesenteric artery from a rat treated with recombinant adenovirus with LacZ (rAdv.LacZ). Arrows indicate the LacZ expression (blue staining) in a mesenteric artery. Right, negative X-gal staining in a mesenteric artery from a rat treated with recombinant adenovirus with rat renin antisense (rAdv.RRA), indicating no LacZ expression (original magnification ×10). Kidney International 2005 68, 680-687DOI: (10.1111/j.1523-1755.2005.00446.x) Copyright © 2005 International Society of Nephrology Terms and Conditions