Figure 2 PGE1 inhibits endocytosis. PGE1 inhibits endocytosis. (A) A 500-ms pulse train with depolarizations from −70 to +10mV (with 300-ms interpulse intervals) followed by ~75s of recording with the cell membrane clamped at −70mV was applied in control and PGE1-treated cells. The entire recordings are presented on the left hand side of the figure, and the pulse-stimulating parts of the traces (marked by dashed box) are shown on the expanded timescale on the right. The arrow on the left indicates the resting cell capacitances before the pulse train. Arrows indicate the experimental conditions (PGE1, black; control, gray). On the left, horizontal lines indicate the starting and minimum levels for determination of the extent of late endocytosis (vertical arrows). The extent of early endocytosis was defined as using ΔCmi values as indicated on the right. (B) A summary of the effect of PGE1 on endocytosis (∆Cm normalized as fF/pF), respectively. (C) A summary of the rates of endocytosis. The early and late phases of endocytosis are indicated as white and gray, respectively. The traces in the figure were averaged from 18 to 21 cells. *P<0.05, **P<0.01. Ying Zhao et al. J Endocrinol 2016;229:287-294 © 2016 Society for Endocrinology