Visualizing Gene Expression

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Figure 1. Characterization of Human U2OS Cells(A) Schematic representation of the gene expression plasmid, p3216PECMS2 . The plasmid is composed.

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Visualizing Gene Expression Steven Henikoff Cell Volume 116, Issue 5, Pages 633-634 (March 2004) DOI: 10.1016/S0092-8674(04)00209-0

Figure 1 Cytological System for Observing Dynamic Changes at an Inducible Locus (A) Cartoon of transgene structure. Lac repressor (LacR) binds and doxycycline induces rtTA, which activates transcription of the CFP-SKL/MS2 binding site chimaeric transcript; this can be visualized with MS2-YFP. (B) Cytological changes that occur at the transgene array upon induction. LacR (red) marks the locus, which begins to unfold in minutes and continues to decondense for an hour or so. Histone H3.3 (green), initially distributed uniformly throughout the nucleus (large oval), accumulates next to LacR within the transgene array and becomes depleted elsewhere, except at nucleoli. These changes at the transgene array follow the accumulation of mRNA and Pol II and accompany the loss of HP1 and H3-K9me. Cell 2004 116, 633-634DOI: (10.1016/S0092-8674(04)00209-0)