IL-11 expression is increased in severe asthma: Association with epithelial cells and eosinophils Eleanor Minshall, PhDa, Jamila Chakir, PhDb, Michel.

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IL-11 expression is increased in severe asthma: Association with epithelial cells and eosinophils Eleanor Minshall, PhDa, Jamila Chakir, PhDb, Michel Laviolette, MDb, Sophie Molet, PhDa, Zhou Zhu, MDc, Ron Olivenstein, MDd, Jack A. Elias, MDc, Qutayba Hamid, MD, PhDa Journal of Allergy and Clinical Immunology Volume 105, Issue 2, Pages 232-238 (February 2000) DOI: 10.1016/S0091-6749(00)90070-8 Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 1 ISH with digoxigenin for IL-11 mRNA in bronchial biopsy specimens from (A) patient with severe asthma and (B) nonasthmatic control subject. Note presence of dark brown/purple staining indicative of IL-11 mRNA within airways epithelium and associated with individual cells in subepithelium. C, Immunostaining for IL-11 in individual with severe asthma. D, Primary antibody isotype control for IL-11 immunoreactivity. E, Colocalization of IL-11 immunoreactivity and eosinophil specific marker (MBP) in individual with severe asthma. IL-11 immunoreactivity is shown as brown coloration that localizes to MBP-positive cells (red staining). F , Collagen (type III) immunoreactivity, as determined by avidin-biotin peroxidase method, in individual with severe asthma is shown as brown staining. Journal of Allergy and Clinical Immunology 2000 105, 232-238DOI: (10.1016/S0091-6749(00)90070-8) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 2 IL-11 mRNA expression within (A) subepithelium and (B) epithelial cell layer of subjects with mild, moderate, and severe asthma and of nonasthmatic control subjects. There was significant increase in numbers of cells expressing IL-11 mRNA in moderate (n = 10) and severe (n = 9) asthma compared with mild asthma (n = 13) and nonasthmatic control subjects (n = 9, asterisk, P < .001 compared with controls and mild asthmatics). Within submucosa, patients with severe asthma had significantly increased numbers of IL-11 mRNA-positive cells compared with those with moderate asthma (pound sign, P < .05). Journal of Allergy and Clinical Immunology 2000 105, 232-238DOI: (10.1016/S0091-6749(00)90070-8) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 3 Correlational relationships between percent predicted FEV1 values and numbers of cells expressing IL-11 mRNA within (A) subepithelium and (B) epithelial cell layer. There were significant correlations between numbers of IL-11 mRNA-positive cells in both airway epithelium and subepithelial regions and this index of pulmonary function (P < .05). Journal of Allergy and Clinical Immunology 2000 105, 232-238DOI: (10.1016/S0091-6749(00)90070-8) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 4 Immunoreactivity for collagens type I and type III in bronchial biopsy specimens from patients with mild, moderate, and severe asthma compared with nonasthmatic control subjects. There was a significant increase in collagen types I and III immunoreactivity in the airways of moderate (n = 10) and severe (n = 9) asthmatics compared with mild asthmatics (n = 13) and nonasthmatic control subjects (n = 9). Asterisk, P < .05 compared with control subjects and those with mild asthma, except for type I collagen where increase in collagen immunoreactivity in moderate asthma was only significant in comparison to healthy control subjects; pound sign, P < .05. Journal of Allergy and Clinical Immunology 2000 105, 232-238DOI: (10.1016/S0091-6749(00)90070-8) Copyright © 2000 Mosby, Inc. Terms and Conditions