Decrease in Epidermal CD44 Expression as a Potential Mechanism for Abnormal Hyaluronate Accumulation in Superficial Dermis in Lichen Sclerosus et Atrophicus

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Decrease in Epidermal CD44 Expression as a Potential Mechanism for Abnormal Hyaluronate Accumulation in Superficial Dermis in Lichen Sclerosus et Atrophicus Gürkan Kaya, Eric Augsburger, Jean-Hilaire Saurat Journal of Investigative Dermatology Volume 115, Issue 6, Pages 1054-1058 (December 2000) DOI: 10.1046/j.1523-1747.2000.00194.x Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Histologic analysis of different epithelial structures in LSA patients and K5-CD44 anti-sense transgenic mice. Tissue sections of uninvolved (A, C) and involved (B, D) back skin of LSA patients and vulvar (E–H) and penile (I–L) epithelium of control littermate (E, G, I, K) and transgenic (F, H, J, L) mice stained with hematoxylin–eosin (A, B, E, F, I, J) and van Gieson–elastin (C, D, G, H, K, L). Note the epidermal atrophy (B, D) and the accumulation of a material not strongly stained in red in the superficial dermis or stroma (B, D, F, H, J, L). Scale bar: 60 μm. Journal of Investigative Dermatology 2000 115, 1054-1058DOI: (10.1046/j.1523-1747.2000.00194.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Analysis of CD44 expression by immunohistochemistry and in situ hybridization in LSA skin. (A, B) immunohistochemistry; (C, D) in situ hybridization. Paraffin-embedded serial skin sections of uninvolved (A, C) and involved (B, D) back skin of LSA patients were stained with anti-human CD44 followed by biotinylated affinity-purified goat anti-rat immunoglobulin antibody (A, B), or hybridized with a DIG-labeled anti-sense mouse CD44H cRNA probe followed by staining with an alkaline phosphatase-conjugated anti-DIG Fab fragment (C, D). Epidermis of uninvolved skin of LSA patients shows an intense immunoreactivity (A) and a strong mRNA hybridization (C), whereas the CD44 staining as well as its RNA is lost in involved epidermis (B, D). Scale bar: 60 μm. Journal of Investigative Dermatology 2000 115, 1054-1058DOI: (10.1046/j.1523-1747.2000.00194.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Alcian blue and hCD44Rg stainings of LSA skin. Alcian Blue staining (A–C) and hCD44Rg reactivity (D–F) in the uninvolved (A, D) and involved (B, C, E, F) back skin from LSA patients either without (A, B, D, E) or with (C, F) pretreatment of the section with 1.5 μg per ml of bovine testicular hyaluronidase. The strong reactivity of the involved skin superficial dermis with Alcian Blue (B) or hCD44Rg (E) is abolished following treatment of the section with hyaluronidase (C, F). Original magnification ×40. Scale bar: 60 μm. Journal of Investigative Dermatology 2000 115, 1054-1058DOI: (10.1046/j.1523-1747.2000.00194.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Alcian blue staining of vulvar and penile epithelium of K5-CD44 anti-sense mice. Alcian Blue staining (A–F) in the sections from vulva (A–C) and penis (D–F) of control littermate (A, D) and transgenic (B, C, E, F) mice either without (A, B, D, E) or with (C, F) pretreatment of the section with 1.5 μg per ml of bovine testicular hyaluronidase. The pronounced reactivity of the transgenic mouse superficial stroma with Alcian Blue (B, E) is abrogated following treatment of the section with hyaluronidase (C, F). Scale bar: 60 μm. Journal of Investigative Dermatology 2000 115, 1054-1058DOI: (10.1046/j.1523-1747.2000.00194.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions