Cristae junction orientation and cristae morphology analyzed by dual-axis electron tomography. Cristae junction orientation and cristae morphology analyzed.

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Cristae junction orientation and cristae morphology analyzed by dual-axis electron tomography. Cristae junction orientation and cristae morphology analyzed by dual-axis electron tomography. Primary adult human fibroblasts were grown on Aclar discs. (A and B) Single slices of the tomogram (grayscale) overlaid with reconstructions of segmented cristae membranes (green). Cristae junctions are denoted by orange spheres. Shown are a top view of a mitochondrion (A) (growth surface in plane with the tomogram) and a side view of the same mitochondrion (B). Note the lamellar cristae and the preferential orientation of the cristae junctions at the sides of the organelle, so that they are in parallel with the growth surface. (C) Magnification of a single slice of the tomogram (top view). The arrows point to the same cristae junctions as marked by the arrows in A. (Scale bar: 100 nm in A and C; 50 nm in B). Jans D C et al. PNAS 2013;110:8936-8941 ©2013 by National Academy of Sciences