ImmunoCAP cellulose displays cross-reactive carbohydrate determinant (CCD) epitopes and can cause false-positive test results in patients with high anti-CCD.

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Presentation transcript:

ImmunoCAP cellulose displays cross-reactive carbohydrate determinant (CCD) epitopes and can cause false-positive test results in patients with high anti-CCD IgE antibody levels Wolfgang Hemmer, PhD, Friedrich Altmann, PhD, Friedrich Holzweber, MD, Clemens Gruber, PhD, Felix Wantke, MD, Stefan Wöhrl, MD, MSc Journal of Allergy and Clinical Immunology Volume 141, Issue 1, Pages 372-381.e3 (January 2018) DOI: 10.1016/j.jaci.2017.04.028 Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Journal of Allergy and Clinical Immunology 2018 141, 372-381 Journal of Allergy and Clinical Immunology 2018 141, 372-381.e3DOI: (10.1016/j.jaci.2017.04.028) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Correlation between IgE binding to CCDs (ImmunoCAP bromelain) and allergen-free ImmunoCAPs coupled with streptavidin (SA-CAP-1) in 52 CCD-positive sera with varying amounts of serum anti-CCD IgE antibodies (1.01-59.6 kUA/L). Journal of Allergy and Clinical Immunology 2018 141, 372-381.e3DOI: (10.1016/j.jaci.2017.04.028) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 IgE binding to allergen-free ImmunoCAPs coupled with streptavidin (SA-CAP-1) in 15 CCD-positive sera before and after serum inhibition with a CCD inhibitor (MUXF3-HSA). Journal of Allergy and Clinical Immunology 2018 141, 372-381.e3DOI: (10.1016/j.jaci.2017.04.028) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Correlation of IgE binding to recombinant allergens with IgE binding to allergen-free streptavidin-CAP (SA-CAP-1; left panel) and bromelain (right panel) in 10 sera with high anti-CCD IgE levels. Journal of Allergy and Clinical Immunology 2018 141, 372-381.e3DOI: (10.1016/j.jaci.2017.04.028) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Identification of N-glycans in processed cotton cellulose. Samples were digested with pepsin in formic acid, and N-glycans were released by PNGase A and analyzed by using MALDI-TOF MS after cation exchange purification, gel filtration, and reverse-phase solid-phase extraction. The main peak at 1211.4 corresponds to complex N-glycans of the MMXF3 type. Peaks marked by asterisks correspond to background signals derived from pepsin. Journal of Allergy and Clinical Immunology 2018 141, 372-381.e3DOI: (10.1016/j.jaci.2017.04.028) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 Dose-dependent inhibition of IgE binding to a streptavidin-coupled dummy CAP (SA-CAP-1; black lines) and bromelain (dotted lines) by a CCD blocker in 3 patients with high anti-CCD IgE levels. Journal of Allergy and Clinical Immunology 2018 141, 372-381.e3DOI: (10.1016/j.jaci.2017.04.028) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 ImmunoCAP IgE binding to Hymenoptera venoms and recombinant venom allergens before and after CCD inhibition in 7 CCD-positive patients with either bee venom allergy (black symbols, n = 2) or yellow jacket venom allergy (white symbols, n = 5) and in a CCD-positive control subject without venom hypersensitivity (gray symbols). +CCD, After CCD inhibition; −CCD, without inhibition; HBV, honeybee venom; YJV, yellow jacket venom. Journal of Allergy and Clinical Immunology 2018 141, 372-381.e3DOI: (10.1016/j.jaci.2017.04.028) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions