Release of tumour necrosis factor α (TNFα) by TNFα cleaving enzyme (TACE) in response to septic stimuli in vitro† Robertshaw H.J. , Brennan F.M. British.

Slides:

Advertisements

Similar presentations

Volume 94, Issue 2, Pages (March 2014)

Advertisements

Maria B. Sukkar, PhD, Shaoping Xie, PhD, Nadia M

Soluble BAFF-R produced by decidual stromal cells plays an inhibitory role in monocytes and macrophages B.P. Deng, Y. Zhang, Q.J. Wang, X.F. Xu, H. Zhang,

Sevoflurane suppresses tumour necrosis factor-α-induced inflammatory responses in small airway epithelial cells after anoxia/reoxygenation K. Watanabe,

Role of γ/δ T cells in a patient with CD4+ CD3– lymphocytosis, hypereosinophilia, and high levels of IgE Ilan Bank, MDa,d, Avner Reshef, MDb, Miriam.

by Rosemary E. Smith, Vanshree Patel, Sandra D. Seatter, Maureen R

Intravital Imaging of IL-1β Production in Skin

Volume 2, Issue 1, Pages (July 2007)

Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.

Volker Assmann, Christina Fieber, Peter Herrlich, Martin Hofmann

Volume 18, Issue 2, Pages (February 2010)

Impaired Responses of Peripheral Blood Mononuclear Cells to Staphylococcal Superantigen in Patients with Severe Atopic Dermatitis: A Role of T Cell Apoptosis

IL-10 disrupts the Brd4-docking sites to inhibit LPS-induced CXCL8 and TNF-α expression in monocytes: Implications for chronic obstructive pulmonary disease

T-cell activation and cytokine production via a bispecific single-chain antibody fragment targeted to blood-stage malaria parasites by Shigeto Yoshida,

Siglec-7 is an inhibitory receptor on human mast cells and basophils

Sphingosylphosphorylcholine is a Potent Inducer of Intercellular Adhesion Molecule-1 Expression in Human Keratinocytes Genji Imokawa, Yutaka Takagi,

Specific Lysis of Melanoma Cells by Receptor Grafted T Cells is Enhanced by Anti- Idiotypic Monoclonal Antibodies Directed to the scFv Domain of the Receptor

Cord Blood CD34+ Cells Differentiate into Dermal Dendritic Cells in Co-Culture with Cutaneous Fibroblasts or Stromal Cells Zia U.A. Mollah, Setsuya Aiba,

Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.

Volume 68, Issue 4, Pages (October 2005)

Human Keratinocytes Express Functional CD14 and Toll-Like Receptor 4

G. D'Agostino, A. Saporito, V. Cecchinato, Y. Silvestri, A. Borgeat, L

Age-Related Differences in Sensitivity of Peripheral Blood Monocytes to Lipopolysaccharide and Staphylococcus Aureus Toxin B in Atopic Dermatitis Marie.

Volume 18, Issue 5, Pages (May 2003)

Volume 87, Issue 2, Pages (February 2015)

Integrin α5β1 Activates the NLRP3 Inflammasome by Direct Interaction with a Bacterial Surface Protein Hye-Kyoung Jun, Sung-Hoon Lee, Hae-Ri Lee, Bong-Kyu.

Volume 140, Issue 1, Pages e3 (January 2011)

Isoflurane affects the cytoskeleton but not survival, proliferation, or synaptogenic properties of rat astrocytes in vitro D.J. Culley, E.K. Cotran,

Volume 73, Issue 1, Pages (January 2008)

Marcel C. Pasch Journal of Investigative Dermatology

IL-31 Induces Chemotaxis, Calcium Mobilization, Release of Reactive Oxygen Species, and CCL26 in Eosinophils, Which Are Capable to Release IL-31 Nikola.

Gangliosides GD1b, GT1b, and GQ1b Suppress the Growth of Human Melanoma by Inhibiting Interleukin-8 Production: the Inhibition of Adenylate Cyclase1

Arming Cytokine-induced Killer Cells With Chimeric Antigen Receptors: CD28 Outperforms Combined CD28–OX40 “Super-stimulation” Andreas A Hombach, Gunter.

Sri Rajalakshmi Rudrabhatla, Christie L. Mahaffey, Mark E. Mummert

Neurotensin receptor–1 and –3 complex modulates the cellular signaling of neurotensin in the HT29 cell line Stéphane Martin, Valérie Navarro, Jean Pierre.

Regulation of pentraxin-3 by antioxidants

Novel Role of the Ras Cascade in Memory B Cell Response

P38 Mitogen-activated Protein Kinase and Extracellular Signal-regulated Kinases Play Distinct Roles in the Activation of Dendritic Cells by Two Representative.

Rolipram Inhibits Polarization and Migration of Human T Lymphocytes

Volume 123, Issue 5, Pages (November 2002)

P38 Mitogen-Activated Protein Kinase Mediates Dual Role of Ultraviolet B Radiation in Induction of Maturation and Apoptosis of Monocyte-Derived Dendritic.

Reciprocal Regulation of Thymus and Activation-Regulated Chemokine/Macrophage- Derived Chemokine Production by Interleukin (IL)-4/IL-13 and Interferon-γ.

Activation and Translocation of p38 Mitogen-Activated Protein Kinase After Stimulation of Monocytes With Contact Sensitizers Pia Brand, Sibylle Plochmann,

Maria C. Lebre, Angelic M. G. van der Aar, Lisa van Baarsen, Toni M. M

Resistance of Human Melanoma Cells Against the Death Ligand TRAIL Is Reversed by Ultraviolet-B Radiation via Downregulation of FLIP Elke Zeise, Michael.

Arsenic Induces Tumor Necrosis Factor α Release and Tumor Necrosis Factor Receptor 1 Signaling in T Helper Cell Apoptosis Hsin-Su Yu, Gwo-Shing Chen

Anti-Mycotics Suppress Interleukin-4 and Interleukin-5 Production in Anti-CD3 Plus Anti- CD28-Stimulated T Cells from Patients with Atopic Dermatitis

Staphylococcal exotoxins are strong inducers of IL-22: A potential role in atopic dermatitis Margarete Niebuhr, MD, Helena Scharonow, MS, Merle Gathmann,

Human CD4+ T Lymphocytes with Remarkable Regulatory Functions on Dendritic Cells and Nickel-Specific Th1 Immune Responses Andrea Cavani, Francesca Nasorri,

IL-12 affects Dermatophagoides farinae–induced IL-4 production by T cells from pediatric patients with mite-sensitive asthma Takeshi Noma, MD, PhD, Izumi.

Αvβ6 Integrin Upregulates Matrix Metalloproteinase 9 and Promotes Migration of Normal Oral Keratinocytes Gareth J. Thomas, S. Poomsawat, Mark P. Lewis,

Ganglioside GQ1b enhances Ig production by human PBMCs

Serotonin Activates Human Monocytes and Prevents Apoptosis

Malondialdehyde oxidation of cartilage collagen by chondrocytes

Volume 42, Issue 4, Pages (April 2015)

Double-Stranded RNA-Exposed Human Keratinocytes Promote Th1 Responses by Inducing a Type-1 Polarized Phenotype in Dendritic Cells: Role of Keratinocyte-Derived.

Pablo F. Peñas, Guadalupe F. Buezo, Luis Rios, Amaro García-Díez

Volume 32, Issue 2, Pages (February 2010)

James Gailit, Mary J. Marchese, Richard R. Kew, Barry L. Gruber

David M. Essayan, MD, Charity C

Harald Renz, MD, Chaya Brodie, PhD, Katherine Bradley, BS, Donald Y. M

Blood eosinophils from atopic donors express messenger RNA for the α, β, and γ subunits of the high-affinity IgE receptor (FcϵRI) and intracellular, but.

Interleukin 6 Indirectly Induces Keratinocyte Migration

Lipopolysaccharide-promoted proliferation of endometriotic stromal cells via induction of tumor necrosis factor α and interleukin-8 expression Yumiko.

Figure 3. Functional and morphological changes in Gal-4-treated monocytes. (A, B) Human monocytes (1×105) were treated with Gal-4 (10 μg/ ml) or LPS (1.

Volume 24, Issue 12, Pages (December 2016)

Sean P. Cullen, Conor J. Kearney, Danielle M. Clancy, Seamus J. Martin

Interleukin-17 is Produced by Both Th1 and Th2 Lymphocytes, and Modulates Interferon-γ- and Interleukin-4-Induced Activation of Human Keratinocytes Cristina.

Volume 18, Issue 2, Pages (February 2010)

Activated protein C inhibits chemotaxis and interleukin-6 release by human neutrophils without affecting other neutrophil functions H.F. Galley, N.E.

Presentation transcript:

Release of tumour necrosis factor α (TNFα) by TNFα cleaving enzyme (TACE) in response to septic stimuli in vitro† Robertshaw H.J. , Brennan F.M. British Journal of Anaesthesia Volume 94, Issue 2, Pages 222-228 (February 2005) DOI: 10.1093/bja/aei021 Copyright © 2005 British Journal of Anaesthesia Terms and Conditions

Fig 1 Changes in memTNF and solTNF in LPS-stimulated monocytes. Human monocytes were plated, stimulated with LPS (10 ng ml−1) and harvested at the time points shown. (a) The cells were fixed with 0.1% paraformaldehyde (final concentration), stained for memTNF (membrane) and analysed by FACS. The data are shown as mean fluorescence intensity (MFI [1 sd]) from seven experiments (appropriate isotype control subtracted). (b) The supernatants were analysed for mean (1 sd) solTNF (soluble) (pg ml−1) by sandwich ELISA (**P<0.01 compared with unstimulated cells). British Journal of Anaesthesia 2005 94, 222-228DOI: (10.1093/bja/aei021) Copyright © 2005 British Journal of Anaesthesia Terms and Conditions

Fig 2 Accumulated memTNF assessed by WEHI assay. Human monocytes were plated and treated with LPS (10 ng ml−1), BB94 (10 μM) and cA2 (100 μg ml−1). The cells were harvested at the time points shown, fixed with 0.1% paraformaldehyde and added to a WEHI assay at a ratio of 10 treated monocytes to one WEHI cell. Data are shown as mean (1 sd) percentage cell death as compared with unstimulated cells from seven experiments, each using a different blood donor. British Journal of Anaesthesia 2005 94, 222-228DOI: (10.1093/bja/aei021) Copyright © 2005 British Journal of Anaesthesia Terms and Conditions

Fig 3 Decrease of surface TACE on LPS-stimulated monocytes. Human monocytes were plated, stimulated with LPS (10 ng ml−1) and harvested at the times shown (a). The cells were fixed with 0.1% paraformaldehyde, stained for TACE and analysed by FACS. The data are shown as MFI (1 sd) from seven experiments (appropriate isotype control subtracted). For confocal microscopy human monocytes were plated, stimulated with LPS and transferred to gelatin-coated coverslips at 24 h. These cells were fixed with 3% paraformaldehyde, blocked with 3% BSA and stained for TACE (M222) and goat anti-mouse IgG-Alexa 488 (10 μg ml−1) as a secondary antibody then visualised by confocal microscopy (b). British Journal of Anaesthesia 2005 94, 222-228DOI: (10.1093/bja/aei021) Copyright © 2005 British Journal of Anaesthesia Terms and Conditions

Fig 4 Changes in memTNF on stimulated monocytes with the addition of BB94. Human monocytes were stimulated with LPS (10 ng ml−1) and harvested at the times shown. To one group of cells BB94 (10 μM) was added at the time of harvest. The cells were fixed with 0.1% paraformaldehyde, stained for memTNF (membrane) and analysed by FACS (10 000 events counted). Data are shown as mean fluorescence intensity (MFI [1 sd]) from seven experiments (appropriate isotype control subtracted). British Journal of Anaesthesia 2005 94, 222-228DOI: (10.1093/bja/aei021) Copyright © 2005 British Journal of Anaesthesia Terms and Conditions

Fig 5 Changes in solTNF and memTNF on monocytes following LPS, zymosan or N. meningitidis stimulation. Human monocytes were stimulated with LPS (10 ng ml−1), zymosan (30 μg ml−1), or heat-inactivated N. meningitidis (106 cells well−1) and harvested at the times shown. (a) Supernatants were removed and analysed for solTNF (soluble) by sandwich ELISA. Data are shown from triplicate cultures using different blood donors and expressed as mean (1 sd) solTNF (pg ml−1). (b) Cells were fixed with 0.1% paraformaldehyde, stained for memTNF (membrane) and analysed by FACS (10 000 events counted). Data are shown as mean fluorescence intensity (MFI [1 sd]) from seven experiments (appropriate isotype control subtracted). British Journal of Anaesthesia 2005 94, 222-228DOI: (10.1093/bja/aei021) Copyright © 2005 British Journal of Anaesthesia Terms and Conditions

Fig 6 Changes in TACE surface protein and catalytic activity in monocytes following LPS, zymosan or N. meningitidis stimulation. Human monocytes were stimulated with LPS (10 ng ml−1), zymosan (30 μg ml−1) or heat-inactivated N. meningitidis (106 cells well−1) and harvested at the times shown. (a) Cells were fixed with 0.1% paraformaldehyde, stained for TACE (membrane) and analysed by FACS (10 000 events counted). (b) To one group of cells BB94 (10 μM) was added at the time of harvest. The cells were stained for memTNF and analysed by FACS (10 000 events counted). Data are shown as mean fluorescence intensity (MFI [1 sd]) from seven experiments (appropriate isotype control subtracted). British Journal of Anaesthesia 2005 94, 222-228DOI: (10.1093/bja/aei021) Copyright © 2005 British Journal of Anaesthesia Terms and Conditions