CD44-Deficient Mice Do Not Exhibit Impairment of Epidermal Langerhans Cell Migration to Lymph Nodes after Epicutaneous Sensitization with Protein Shi-Chuen.

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CD44-Deficient Mice Do Not Exhibit Impairment of Epidermal Langerhans Cell Migration to Lymph Nodes after Epicutaneous Sensitization with Protein Shi-Chuen Miaw, Jau-Shiuh Chen, Pei-Chen Hsieh, Ching-Yi Liu, Ming-Hui Chung, Pei-Chun Chen, Mei-Ling Chang, Fen-Ling Chen, John T. Kung, Li-Fang Wang Journal of Investigative Dermatology Volume 130, Issue 11, Pages 2674-2677 (November 2010) DOI: 10.1038/jid.2010.170 Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Numbers of dendritic cells (DCs) in draining lymph nodes (LNs). (a) CD44 staining of peripheral blood leucocytes of CD44+/+ (dash), CD44P256/+ (dotted), and CD44P256/P256 (solid) mice, and unstained control (shaded) are shown. (b) All CD44 exons were subjected to DNA sequencing, and a T-to-A transversion mutation within exon 2 was found (asterisk denotes premature termination). (c) Reverse transcriptase-PCR amplification between exon 1 and exon 5. (d, e, g) Draining LNs were isolated from naive mice (d) or 96hours after immunization (e, g). CD11c+ cells were gated. Results are expressed as numbers per six draining LNs per mouse. The pooled data of three to five experiments are shown. (f) LNs were isolated from CD44-deficient mice 96hours after patch application of ovalbumin (OVA) (dash) or OVA-DQ (solid). Langerin+ cells were gated. (g) Langerinhigh CD103− DCs were gated. Journal of Investigative Dermatology 2010 130, 2674-2677DOI: (10.1038/jid.2010.170) Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 T-cell responses in CD44-deficient and wild-type C57BL/6 mice. Groups of CD44-deficient or wild-type mice were immunized by (a) patch application with ovalbumin (OVA) solution or phosphate-buffered saline control for 5 successive days or (b) subcutaneous injection with OVA plus complete Freund's adjuvant on day 1. At 10 days after immunization, draining lymph nodes (LNs) were isolated and in vitro OVA reactivation cultures were done. IFN-γ, IL-5, and IL-13 levels in 48-hour supernatants were determined by ELISA. Net concentration (concentration in the absence of OVA subtracted from the concentration in the presence of OVA) is shown. Representative results from three independent experiments are shown. Journal of Investigative Dermatology 2010 130, 2674-2677DOI: (10.1038/jid.2010.170) Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions