Volume 130, Issue 3, Pages 1019-1021 (March 2006) Correction    Gastroenterology  Volume 130, Issue 3, Pages 1019-1021 (March 2006) DOI: 10.1053/j.gastro.2006.02.001 Copyright © 2006 American Gastroenterological Association Terms and Conditions

Figure 5 The GLP-2R protein localized to VIP-positive enteric neurons (EN) and eNOS-expressing EN in the whole-mount submucosal preparation of the porcine jejunum by double immunostaining. The GLP-2R protein was immunostained by the hGLP-2R antibody (in green; A, D, and G). The enteric neurons were immunostained by antibodies against a neuron-specific marker (PGP 9.5, in red; B), a specific vasoneurotransmitter (VIP, in red; E), or eNOS protein (eNOS, in red; H). The GLP-2R protein was coexpressed (in yellow, indicated by arrows) with PGP 9.5 (C), VIP (F), and eNOS (I). Note that representative images are shown of enteric neurons within the ganglia of the submucosal plexus of the neonatal porcine jejunum. Gastroenterology 2006 130, 1019-1021DOI: (10.1053/j.gastro.2006.02.001) Copyright © 2006 American Gastroenterological Association Terms and Conditions

Figure 6 The GLP-2R protein localized to 5-HT-containing enteroendocrine cells (EC) of the human jejunum by double immunostaining. The hGLP-2R protein was expressed in EC (in yellow, indicated by arrows) scattered in villus epithelium (A) and crypt epithelium (E) and expressed in 5-HT-containing EC (I). Note also that not all EC (in red, indicated by arrowheads) were GLP-2R immunoreactive. hGLP-2R protein was immunostained by the hGLP-2R antibody (in green; B, F, and J); the EC were labeled by anti-chromogranin A (Chro A) antibody (in red; C and G); and 5-HT-containing EC were labeled by anti-5-HT antibody (in red; K). hGLP-2R protein was coexpressed with the EC-specific marker (Chro A, in yellow; D and H), specifically with a 5-HT-containing subset of EC (5-HT, in yellow; L). Nuclei (DNA) were counterstained by TOPRO-3 (in blue). White squares (in A, E, and I) are enlarged for details (as shown in B–D, F–H, and J–L; respectively). Gastroenterology 2006 130, 1019-1021DOI: (10.1053/j.gastro.2006.02.001) Copyright © 2006 American Gastroenterological Association Terms and Conditions

Figure 7 The GLP-2R protein localized to VIP-positive enteric neurons (EN) and eNOS-expressing EN of the human jejunum by double immunostaining. hGLP-2R protein was expressed in EN (in yellow; A) and coexpressed with VIP (in yellow; E) and eNOS (in yellow; I). However, not all hGLP-2R immunoreactivity was VIP positive (in green, indicated by arrows; E), and not all PGP 9.5 immunoreactivity was eNOS positive (in green; indicated by an arrow; M). hGLP-2R protein was immunostained by the hGLP-2R antibody (in green; B, F, and J). The EN were labeled by a general neuron marker (PGP 9.5; C and O) and a specific neurotransmitter (VIP; G). A subset of cells was immunostained by mouse anti-eNOS antibody (in red; K and N). hGLP-2R protein was coexpressed with the neuronal marker (PGP 9.5, in yellow; D), the neurotransmitter (VIP, in yellow; H), and the eNOS protein (in yellow; I) that was colocalized with the neuronal marker (PGP 9.5, in yellow; P). Nuclei (DNA) were counterstained by TOPRO-3 (in blue). White squares (in A, E, I, and M) are enlarged for details (as shown in B–D, F–H, J–L, and N–P; respectively). Gastroenterology 2006 130, 1019-1021DOI: (10.1053/j.gastro.2006.02.001) Copyright © 2006 American Gastroenterological Association Terms and Conditions