Isaac Perea-Gil et al. BTS 2016;1:

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Isaac Perea-Gil et al. BTS 2016;1:360-372 Determination of Collagen Content and Composition in the Infarct Core (A and B) Representative bright light microscopy images with sirius red staining distinguishing collagen fibers (red) and healthy myocardium (yellow/orange) from EMG-cell free and EMG-ATDPC animals, respectively. (C and D) Polarized light microscopy images with sirius red staining showing type I collagen (red/yellow) and type-III collagen (green) in the EMG-cell free and EMG-ATDPC groups, respectively. Scale bars = 50 μm. (E) Collagen percentage—expressed as collagen volume fraction (CVF [%], left y-axis)—for total collagen, type I collagen (Col I), and type III collagen (Col III); as well as the ratio between type I and III collagens (Col I/III ratio, right y-axis), for both the EMG-cell free and EMG-ATDPC groups. Results are displayed as mean ± SEM. *p = 0.016, †p = 0.002, ‡p = 0.042, §p = 0.032. Abbreviations as in Figure 1. Isaac Perea-Gil et al. BTS 2016;1:360-372 The Authors