Chlamydia trachomatis and human papillomavirus infection in Indian women with sexually transmitted diseases and cervical precancerous and cancerous lesions

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Chlamydia trachomatis and human papillomavirus infection in Indian women with sexually transmitted diseases and cervical precancerous and cancerous lesions V. Gopalkrishna, N. Aggarwal, V.L. Malhotra, R.V. Koranne, V.P. Mohan, A. Mittal, B.C. Das Clinical Microbiology and Infection Volume 6, Issue 2, Pages 88-93 (February 2000) DOI: 10.1046/j.1469-0691.2000.00024.x Copyright © 2000 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 1a Detection of Chlamydia trachomatis plasmid-specific amplification by polymerase chain reaction. Lane 1 is positive control for C. trachomatis and lanes 2-8 are the cases. Lanes 3, 4, 6 and 8 show amplification of a C. trachomatis specific 201 bp product along with 268 bp amplimer for the β-globin gene as internal control. Lanes 2, 5 and 7 are negative for chlamydia showing amplification of only β-globin gene but absence of chlamydia-specific amplimer. The extreme left lane is Hae III-digested ø×174 DNA size marker. Clinical Microbiology and Infection 2000 6, 88-93DOI: (10.1046/j.1469-0691.2000.00024.x) Copyright © 2000 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 1b Polymerase chain reaction amplification of human papillomavirus type 16 (HPV16) DNA along with β-globin gene sequences in endocervical scrapes of patients with sexually transmitted diseases. First lane is Hae III digested ø× 174 DNA size marker. Lane 1 is placental DNA showing β-globin gene (268bp) amplification. Lane 2 is Cacx DNA showing HPV16 gene (217) amplification. Lane 3-5 are endocervical scrapes showing positivity of HPV16 and β-globin gene while lane 6 is negative for HPV16. Clinical Microbiology and Infection 2000 6, 88-93DOI: (10.1046/j.1469-0691.2000.00024.x) Copyright © 2000 European Society of Clinical Infectious Diseases Terms and Conditions