Immunoreactive secreted proteins from clones of S

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Immunoreactive secreted proteins from clones of S Immunoreactive secreted proteins from clones of S. zooepidemicus from outbreaks of pneumonia in dogs and greyhounds in different regions of the United States. Immunoreactive secreted proteins from clones of S. zooepidemicus from outbreaks of pneumonia in dogs and greyhounds in different regions of the United States. Proteins secreted in overnight cultures in THB were precipitated with 50% ammonium sulfate, dissolved in PBS, and transferred to nitrocellulose membranes following SDS-PAGE (12%). The blot was probed with convalescent-phase serum (1:200) from Angel (Pennsylvania), infected with ST-315. Lane 1, S. zooepidemicus strain 007 (Kansas); lane 2, S. zooepidemicus strain 985 (Kansas); lane 3, S. zooepidemicus strain 43 (Florida); lane 4, S. zooepidemicus strain 2201 (Texas); lane 5, S. zooepidemicus strain 2295 (Texas); lane 6, S. zooepidemicus strain 800 (New Mexico); lane 7, S. zooepidemicus strain 1150K (Nevada); lane 8, S. zooepidemicus strain 653 (Pennsylvania). Molecular masses (kDa) are shown on the left. The positions of metalloproteinase/mucinase (115 kDa) and enolase (47 kDa) are shown on the right. The positions of metalloproteinase/mucinase (115 kDa) and enolase (47 kDa) are shown on the right. Sridhar Velineni et al. Clin. Vaccine Immunol. 2014;21:1246-1252