Skin CD4+ T Cells Produce Interferon-γIn Vitro in Response to Streptococcal Antigens in Chronic Plaque Psoriasis  Dean W. Brown, Barbara S. Baker, Jean-Marc Ovigne, Catherine Hardman, Anne V. Powles, Lionel Fry  Journal of Investigative Dermatology  Volume 114, Issue 3, Pages 576-580 (March 2000) DOI: 10.1046/j.1523-1747.2000.00919.x Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Strep-A-induced IFN-γ production by skin CD4+ T cells in psoriasis. A psoriatic skin TCL was stained for surface CD4 or CD8 and intracellular IFN-γ expression, and analyzed by flow cytometry. The TCL was cultured with Strep-A (A, B), or in the absence of Strep-A (C, D). Strep-A induced IFN-γ production by CD4+, but not CD8+, T cells (A2 compared with B2). These dot blots are representative of the results obtained for eight out of 10 psoriatic skin TCL. Journal of Investigative Dermatology 2000 114, 576-580DOI: (10.1046/j.1523-1747.2000.00919.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Strep-A induces IFN-γ production by CD4+ T cells in psoriatic, but not disease control, skin TCL. Skin TCL were stained as described in the caption to Figure 1. The difference in the percentage of CD4+ and CD8+ IFN-γ- producing T cells after culture with, compared to culture without, streptococcal antigens is shown for each patient (1–10 psoriasis, A-E disease controls). Journal of Investigative Dermatology 2000 114, 576-580DOI: (10.1046/j.1523-1747.2000.00919.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Minimal increase in IL-4+ T cells in response to Strep-A in psoriatic and disease control skin TCL. Skin TCL were stained for surface CD4 or CD8 and intracellular IL-4 expression and were analyzed by flow cytometry. The difference in the percentage of CD4+ and CD8+ IL-4-producing T cells after culture with, compared to culture without, streptococcal antigens is shown for psoriatic patients 5, 6, 7, 8, and 10 and disease control patients A-E. (These TCL correspond to those with the same number in Figure 2.) Journal of Investigative Dermatology 2000 114, 576-580DOI: (10.1046/j.1523-1747.2000.00919.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Minimal decrease in IL-10+ T cells in response to Strep-A in psoriatic and disease control skin TCL. Skin TCL were stained for surface CD4 or CD8 and intracellular IL-10 expression and were analyzed by flow cytometry. The difference in the percentage of CD4+ and CD8+ IL-10-producing T cells after culture with, compared to culture without, streptococcal antigens is shown for psoriatic patients 1, 2, 3, 4, and 10 and disease control patients A-D. (These TCL correspond to those with the same number in Figure 2.) Journal of Investigative Dermatology 2000 114, 576-580DOI: (10.1046/j.1523-1747.2000.00919.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions