A possible novel Francisellagenomic species isolated from blood and urine of a patient with severe illness R. Escudero, M. Elía, J.A. Sáez-Nieto, V.

Slides:

Advertisements

Similar presentations

Models for the organisation of hospital infection control and prevention programmes B. Gordts Clinical Microbiology and Infection Volume 11, Pages

Advertisements

Molecular characterisation of Haemophilus influenzae type a and untypeable strains isolated simultaneously from cerebrospinal fluid and blood: novel use.

Molecular and phenotypic characterization of invasive group B streptococcus strains from infants in Norway 2006–2007 H. Bergseng, J.E. Afset, A. Radtke,

Helicobacter pylori resistance to antibiotics in 2014 in France detected by phenotypic and genotypic methods A. Ducournau, L. Bénéjat, E. Sifré, E. Bessède,

Characteristics of Clostridium difficile strains isolated from asymptomatic individuals and from diarrheal patients S. Wongwanich, P. Pongpech, C. Dhiraputra,

Stomatococcus mucilaginosus septicemia in leukemic patients

S. M. Kotila, A. Virolainen, M. Snellman, S. Ibrahem, J. Jalava, O

L. Skjøt-Rasmussen, S. S. Olsen, L. Jakobsen, K. Ejrnæs, F. Scheutz, B

G. L. Daikos, P. Triantafyllopoulou, V. Syriopoulou, D

A. Moya, R. Gil, A. Latorre Clinical Microbiology and Infection

K. Diestra, E. Miró, C. Martí, D. Navarro, J. Cuquet, P. Coll, F

Increased conjugation frequencies in clinical Enterococcus faecium strains harbouring the enterococcal surface protein gene esp B. Lund, H. Billström,

A.K. Reddy, P. Garg, I. Kaur Clinical Microbiology and Infection

Evidence of transmission of verocytotoxin-producing Escherichia coli O111 from a cattle stable to a child E. Møller Nielsen, C. Jensen, D. Lau Baggesen

Molecular and phenotypic profiling of sorbitol-fermenting Escherichia coli O157:H− human isolates from Finland M. Eklund, M. Bielaszewska, U.-M. Nakari,

A PCR-based method to differentiate between Acinetobacter baumannii and Acinetobacter genomic species 13TU P.G. Higgins, H. Wisplinghoff, O. Krut, H.

D.S. Hansen, R. Skov, J.V. Benedí, V. Sperling, H.J. Kolmos

Risk factors and outcome for colistin-resistant Acinetobacter nosocomialis bacteraemia in patients without previous colistin exposure Y.-C. Wang, Y.-T.

L. Skjøt-Rasmussen, S. S. Olsen, L. Jakobsen, K. Ejrnæs, F. Scheutz, B

Genome-based insights into the evolution of enterococci

Phenotypic and molecular characterization of multidrug-resistant Salmonella enterica serovar Hadar in Greece, from 2007 to 2010 T. Papadopoulos, E. Petridou,

Klebsiella pneumoniae: development of a mixed population of carbapenem and tigecycline resistance during antimicrobial therapy in a kidney transplant.

Antibiotic susceptibility and genotypic characterization of Haemophilus influenzae strains isolated from nasopharyngeal specimens from children in day-care.

A cluster of Mycobacterium massiliense keratitis in foundry workers

M. Sabria, J. Alvarez, A. Dominguez, A. Pedrol, G. Sauca, L

Breast milk causing neonatal sepsis and death

Use of sequence-based typing and multiplex PCR to identify clonal lineages of outbreak strains of Acinetobacter baumannii J.F. Turton, S.N. Gabriel,

A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCCmec types I–V K. Boye, M.D. Bartels, I.S. Andersen, J.A. Møller,

Development of antimicrobial resistance in Staphylococcus lugdunensis during treatment—report of a case of bacterial arthritis, vertebral osteomyelitis.

Phenotypic and genotypic characterization of Neisseria gonorrhoeae in parts of Italy: detection of a multiresistant cluster circulating in a heterosexual.

Emergence of CTX-M-15 extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolates in Bosnia and Herzegovina A. Dedeic-Ljubovic, M. Hukic,

Panton–Valentine leukocidin-positive Staphylococcus aureus skin and soft tissue infections among children in an emergency department in Madrid, Spain

Multiple genotypes and subtypes of hepatitis B and C viruses in Belarus: similarities with Russia and western European influences C.M. Olinger, N.V.

Ciprofloxacin-resistant, CTX-M-15-producing Escherichia coli ST131 clone in extraintestinal infections in Italy M. Cerquetti, M. Giufrè, A. García-Fernández,

Ehrlichia canis phylogenetic analysis of the Borgo (Corsica) strain

S.-M. Sheu, B.-S. Sheu, C.-C. Lu, H.-B. Yang, J.-J. Wu

Metagenomics and probiotics

Neisseria meningitidis W-135 in the Basque Country, northern Spain

Detection of Wolbachia genes in a patient with non-Hodgkin's lymphoma

S. Vourli, H. Tsorlini, H. Katsifa, M. Polemis, L. S. Tzouvelekis, A

Two chromosomally located qnrB variants, qnrB6 and the new qnrB16, in Citrobacter spp. isolates causing bacteraemia J. Sanchez-Cespedes, S. Marti, S.M.

R. Alonso, C. Muñoz, T. Peláez, E. Cercenado, M. Rodríguez-Creixems, E

A. Mazón, A. Gil-Setas, L. J. Sota de la Gándara, A. Vindel, J. A

Nationwide pseudo-outbreak of Salmonella enterica ssp

J. Price, N. Claire Gordon, D. Crook, M. Llewelyn, J. Paul

Update on antifungal resistance in Aspergillus and Candida

B.J.A. Rijnders, E. van Wijngaerden, J. van Eldere, W.E. Peetermans

The dominant methicillin‐resistant Staphylococcus aureus clone from hospitals in Cape Town has an unusual genotype: ST612 M.J. Jansen van Rensburg, V.

C. Oprica, C.E. Nord Clinical Microbiology and Infection

A. McNally, F. Alhashash, M. Collins, A. Alqasim, K. Paszckiewicz, V

Human isolates of Aeromonas possess Shiga toxin genes (stx1 and stx2) highly similar to the most virulent gene variants of Escherichia coli A. Alperi,

S.B. Schwartz, K.A. Thurman, S.L. Mitchell, B.J. Wolff, J.M. Winchell

A.P. Underwood, J. Green Clinical Microbiology and Infection

Genetic relatedness between group B streptococci originating from bovine mastitis and a human group B streptococcus type V cluster displaying an identical.

M. J. Medina-Pascual, S. Valdezate, G. Carrasco, P. Villalón, N

S. Figueiredo, L. Poirel, A. Papa, V. Koulourida, P. Nordmann

Methicillin-resistant Staphylococcus saprophyticus in Sweden carries various types of staphylococcal cassette chromosome mec (SCCmec) B. Söderquist,

S. Alexiou-Daniel, A. Stylianakis, A. Papoutsi, I. Zorbas, A. Papa, A

Comparison of one-tube multiplex PCR, automated ribotyping and intergenic spacer (ITS) sequencing for rapid identification of Acinetobacter baumannii

Evolutionary relationships between sporadic and epidemic strains of healthcare- associated methicillin-resistant Staphylococcus aureus M. Hallin, O. Denis,

B. Bakhshi, M.R. Pourshafie, F. Navabakbar, A. Tavakoli

C. Novais, T.M. Coque, J.C. Sousa, L.V. Peixe

Predominance of SHV-5 β-lactamase in enteric bacteria causing community-acquired urinary tract infections in Bosnia and Herzegovina S. Uzunovic-Kamberovic,

Staphylococcus lugdunensis: clinical spectrum, antibiotic susceptibility, and phenotypic and genotypic patterns of 39 isolates C. Hellbacher, E. Törnqvist,

Emergence of Pseudomonas aeruginosa strains producing metallo-β-lactamases of the IMP-15 and VIM-2 types in Mexico F. Quinones-Falconi, M. Galicia-Velasco,

G.C. Schito Clinical Microbiology and Infection

Typing of Clostridium difficile

D.A. Robinson, M.C. Enright Clinical Microbiology and Infection

Impact of antibiotic restrictions: the patient's perspective

The future of diagnostic bacteriology

Presentation transcript:

A possible novel Francisellagenomic species isolated from blood and urine of a patient with severe illness R. Escudero, M. Elía, J.A. Sáez-Nieto, V. Menéndez, A. Toledo, G. Royo, M. Rodríguez-Vargas, M.J. Whipp, H. Gil, I. Jado, P. Anda Clinical Microbiology and Infection Volume 16, Issue 7, Pages 1026-1030 (July 2010) DOI: 10.1111/j.1469-0691.2009.03029.x Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Phenotypic and genotypic characteristics of the isolate FhSp1 as compared with other members of the genus Francisella. (a) Protein profile in 12% acrylamide gels. (b) Immunoblot analysis with the patient's serum. (c) Amplification of the SSTR9 element. (d) pulsed-field gel electrophoresis analysis of PmEI-digested genomic DNA. Arrows indicate bands cited in the text. (a) Lane 1: Francisella tularensis ssp. holarctica (LVS). Lane 2: F. tularensis ssp. tularensis (B38). Lane 3: Francisella novicida F×1. Lane 4: F. novicida F×2. Lane 5: F. novicida (U112). Lane 6: isolate FhSp1. Lane 7: Francisella philomiragia (CCUG 4992). (b) Lane 1: F. philomiragia (CCUG 4992). Lane 2: F. tularensis ssp. tularensis (B38). Lane 3: F. tularensis ssp. holarctica (LVS). Lane 4: F. novicida (U112). Lane 5: isolate FhSp1. (c) Lane 1: F. tularensis ssp. holarctica (LVS). Lane 2: F. tularensis ssp. tularensis (B38). Lane 3: F. novicida (U112). Lane 4: isolate FhSp1. Lane 5: F. novicida F×1. (d) Lane 1: F. tularensis ssp. holarctica (LVS). Lane 2: F. tularensis ssp. tularensis (B38). Lane 3: F. novicida F×1. Lane 4: F. novicida F×2. Lane 5: F. novicida (U112). Lane 6: isolate FhSp1. Lane 7: F. philomiragia (CCUG 4992). Clinical Microbiology and Infection 2010 16, 1026-1030DOI: (10.1111/j.1469-0691.2009.03029.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Phylogenetic analyses of 16S rRNA (a) and a multilocus concatenatedsequence analysis (b). In (a), the GenBank accession numbers used are indicated, and for (b), the following sequences were used: AM261086–AM261089, AM261091, AM261093, AM261097, AM261099 and EU683021 for dnaA, AM261181 to AM261183, AM261186 to AM261188, AM261194, AM261196 and EU682965 for mutS; AM261118–AM261120, AM261123, AM261125, AM261127, AM261128, AM261133 and AM261203 for prfB; AM261165, AM261167, AM261168, AM261170, AM261177–AM261179, AM261180 and EU682989 for putA; and AM261105, AM261115–AM261117, AM261200, AM261202, AM261203, AM261206 and AM261209 for tpiA. For strains WY96-3418 and FTA, the complete genome sequence was used (CP000608 and CP000803, respectively). The percentages shown are those of the identity of FhSp1 with the rest of the strains. Clinical Microbiology and Infection 2010 16, 1026-1030DOI: (10.1111/j.1469-0691.2009.03029.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions