AGE-Modified Collagens I and III Induce Keratinocyte Terminal Differentiation through AGE Receptor CD36: Epidermal–Dermal Interaction in Acquired Perforating.

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AGE-Modified Collagens I and III Induce Keratinocyte Terminal Differentiation through AGE Receptor CD36: Epidermal–Dermal Interaction in Acquired Perforating Dermatosis Eita Fujimoto, Takashi Kobayashi, Norihiro Fujimoto, Minoru Akiyama, Shingo Tajima, Ryoji Nagai Journal of Investigative Dermatology Volume 130, Issue 2, Pages 405-414 (February 2010) DOI: 10.1038/jid.2009.269 Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Expression of involucrin and keratin 10 in response to unmodified or advanced glycation end product (AGE)-modified extracellular matrix (ECM) substrates in cultured keratinocytes. Keratinocytes were grown on unmodified or AGE-modified collagens (col) I, III, IV, and V; fibronectin (FN); or laminin (LN) for 12, 24, and 48hours. RNA was isolated from the cells, and real-time PCR was performed that was directed to the involucrin and keratin 10 mRNAs. Values are expressed as the ratio of AGE-modified substrates (closed columns) relative to the unmodified substrates (open columns). Numbers are mean±SD (n=6). *Statistical significance at P<0.05. Journal of Investigative Dermatology 2010 130, 405-414DOI: (10.1038/jid.2009.269) Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Expression of advanced glycation end product (AGE) receptors in response to AGE-modified collagens (col) I and III or fibronectin (FN) substrates in cultured keratinocytes. Keratinocytes were cultured on unmodified or AGE-modified collagens I and III or on FN substrate for 12, 24, and 48hours. Relative mRNA levels of CD36, galectin-3 (Gal-3), receptor for advanced glycation end product (RAGE), and class B scavenger receptor type I (SR-BI) were determined and presented as described in Figure 1. Numbers are mean±SD (n=6). *Statistical significance at P<0.05. Journal of Investigative Dermatology 2010 130, 405-414DOI: (10.1038/jid.2009.269) Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Effect of neutralizing advanced glycation end product (AGE) receptor antibodies on glycated collagen-induced involucrin expression. Keratinocytes were cultured on unmodified or AGE-modified collagen I substrates for 48hours in the presence or absence of anti-CD36 or anti-RAGE neutralizing antibody at 10 or 25μgml−1. (a) RNA was isolated and the involucrin mRNA level was determined by real-time PCR. Values are mean±SD (n=6); *Indicates statistical significance at P<0.05. (b) Keratinocytes were incubated on AGE-modified collagen I for 0 hour (A) or 48 hours without neutralizing Abs (B) or with neutralizing Abs for CD36 (C) or RAGE (D). Cells were immunoreacted with anti-involucrin antibody (left). Involucrin-positive cells were counted from the observation of six random, nonoverlapping fields. Values are mean±SD (n=6) (right). Bar = 50μm. *Statistical significance at P<0.05. RAGE, receptor for advanced glycation end product. Journal of Investigative Dermatology 2010 130, 405-414DOI: (10.1038/jid.2009.269) Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Expression of advanced glycation end product (AGE) receptors during suspension culture–induced terminal differentiation. Keratinocytes were trypsinized and suspended for 0, 24, and 48hours in 1.5% methylcellulose. (a) RNA was extracted from the cell pellet. AGE receptor and involucrin mRNA levels were determined by real-time PCR. (b) Cells were placed on slide glasses, fixed with acetone, and incubated with the anti-involucrin (red) and anti-CD36 (green) antibodies. Bound antibody was visualized with fluorescence-conjugated secondary antibodies. Bars = 50μm. Gal-3, galectin-3; RAGE, receptor for advanced glycation end product; SR-BI, class B scavenger receptor type I. Journal of Investigative Dermatology 2010 130, 405-414DOI: (10.1038/jid.2009.269) Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Expression of matrix metalloproteinase-2 (MMP-2) and MMP-9 by cultured keratinocytes grown on advanced glycation end product (AGE)-modified collagens I, III, and IV and fibronectin (FN) substrates. (a) Keratinocytes were cultured on unmodified and AGE-modified collagens (col) (I, III, and IV) and FN for 12, 24, and 48hours. RNA was isolated, and the MMP-2 and MMP-9 mRNA levels were determined by real-time PCR. (b) Keratinocytes were cultured on unmodified and AGE-modified collagen I for 12, 24, and 48hours. Cultured media were recovered from the cells. Proteins were precipitated with 10% trichloroacetic acid. MMP-2 and MMP-9 levels were measured with one-step sandwich enzyme-linked immunoassay using an mAb kit (left and middle). The proteins were resolved on 7.5% SDS-PAGE containing 0.5% gelatin. Gelatin zymography was performed at 35°C for 3hours (right). Journal of Investigative Dermatology 2010 130, 405-414DOI: (10.1038/jid.2009.269) Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 6 Immunostaining of the lesional skin obtained from patients with acquired perforating dermatosis using antibodies for the advanced glycation end product (AGE) receptors (CD36, SR-A, and RAGE) and matrix metalloproteinase 9 (MMP-9). Normal skin corresponding to case 8 in Table 1 (upper panel) and lesional skin of patients corresponding to cases 1 and 2 in Table 1 (middle and lower panels) were fixed with paraffin, sliced at 5-μm thickness, and incubated with antibodies (1:200) for CD36, macrophage class A scavenger receptor (SR-A), receptor for advanced glycation end product (RAGE), and MMP-9 overnight at 4°C. The sections were incubated with anti-mouse, -rabbit, or -goat Ig antibody at 1:600 for 1hour. The antigen–antibody complex was visualized by the avidin–biotin complex. Bars=200μm. D, dermis; E, epidermis; M, extruded material. Journal of Investigative Dermatology 2010 130, 405-414DOI: (10.1038/jid.2009.269) Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 7 Summary of the molecular mechanism of transepidermal elimination (TEE). Hypothesis of the development of TEE of advanced glycation end product (AGE)-modified collagen in patients with acquired perforating dermatosis associated with diabetes mellitus and renal insufficiency. BMZ, basement membrane zone. Journal of Investigative Dermatology 2010 130, 405-414DOI: (10.1038/jid.2009.269) Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions