Evaluation of a single-tube real-time PCR for detection and identification of 11 dermatophyte species in clinical material A.M.C. Bergmans, M. van der.

Slides:

Advertisements

Similar presentations

Models for the organisation of hospital infection control and prevention programmes B. Gordts Clinical Microbiology and Infection Volume 11, Pages

Advertisements

C.-S. Lee, J.-H. Lee Clinical Microbiology and Infection

P.-Y. Lévy Clinical Microbiology and Infection

Detection of intestinal protozoa in paediatric patients with gastrointestinal symptoms by multiplex real-time PCR L. Maas, J.W. Dorigo-Zetsma, C.J. de.

Validation of a four-primer real-time PCR as a diagnostic tool for single and mixed Plasmodium infections L. Cnops, J. Jacobs, M. Van Esbroeck Clinical.

R. Dumke, H. von Baum, P.C. Lück, E. Jacobs

A. Bergman, D. Heimer, N. Kondori, H. Enroth

Rapid single-nucleotide polymorphism-based identification of clonal Pseudomonas aeruginosa isolates from patients with cystic fibrosis by the use of real-time.

Detection of Panton–Valentine leukocidin gene in Staphylococcus aureus by LightCycler PCR: clinical and epidemiological aspects D. Johnsson, P. Mölling,

The agony of choice in dermatophyte diagnostics—performance of different molecular tests and culture in the detection of Trichophyton rubrum and Trichophyton.

Development of a single tube multiplex real-time PCR to detect the most clinically relevant Mucormycetes species L. Bernal-Martínez, M.J. Buitrago, M.V.

Detection of fungal DNA in lysis–centrifugation blood culture for the diagnosis of invasive candidiasis in neonatal patients L. Trovato, P. Betta, M.G.

Validation of PCR–reverse line blot, a method for rapid detection and identification of nine dermatophyte species in nail, skin and hair samples A.M.C.

Gut bacterial microbiota and obesity

Clinical significance of signal pattern of high-risk human papillomavirus using a novel fluorescence in situ hybridization assay in cervical cytology

Simultaneous single-tube PCR assay for the detection of Neisseria meningitidis, Haemophilus influenzae type b and Streptococcus pneumoniae G. Tzanakaki,

C.-S. Lee, J.-H. Lee Clinical Microbiology and Infection

A.K. Reddy, P. Garg, I. Kaur Clinical Microbiology and Infection

J.-P. Van geertruyden Clinical Microbiology and Infection

Multi-Probe Real-Time PCR Identification of Common Mycobacterium Species in Blood Culture Broth Suporn Foongladda, Suporn Pholwat, Boonchuay Eampokalap,

A comparison of three real-time PCR assays for the confirmation of Neisseria gonorrhoeae following detection of N. gonorrhoeae using Roche COBAS AMPLICOR

Detection of Panton–Valentine leukocidin gene in Staphylococcus aureus by LightCycler PCR: clinical and epidemiological aspects D. Johnsson, P. Mölling,

B.J. Kocjan, D. Bzhalava, O. Forslund, J. Dillner, M. Poljak

G. Zhao, H. Zhai, Q. Yuan, S. Sun, T. Liu, L. Xie

Validation of PCR–reverse line blot, a method for rapid detection and identification of nine dermatophyte species in nail, skin and hair samples A.M.C.

R. Cantón Clinical Microbiology and Infection

A PCR-based method to differentiate between Acinetobacter baumannii and Acinetobacter genomic species 13TU P.G. Higgins, H. Wisplinghoff, O. Krut, H.

Public health significance of invasive mosquitoes in Europe

B.J. Kocjan, D. Bzhalava, O. Forslund, J. Dillner, M. Poljak

P. Balraj, G. Vestris, D. Raoult, P. Renesto

Development and evaluation of a multiplex test for the detection of atypical bacterial DNA in community-acquired pneumonia during childhood J.A. Carrillo,

Extraction of Chlamydia pneumoniae DNA from vascular tissue for use in PCR: an evaluation of four procedures H.F. Berg, B. Maraha, A.M.C. Bergmans, A.

Evaluation of differential gene expression in susceptible and resistant clinical isolates of Klebsiella pneumoniae by DNA microarray analysis A. Doménech-Sánchez,

Rapid diagnosis of human brucellosis by SYBR Green I-based real-time PCR assay and melting curve analysis in serum samples M.I. Queipo-Ortuño, J.D. Colmenero,

How to evaluate and predict the ecologic impact of antibiotics: the pharmaceutical industry view from research and development R. Bax Clinical Microbiology.

Should standardized susceptibility testing for microbial biofilms be introduced in clinical practice? T. Coenye, D. Goeres, F. Van Bambeke, T. Bjarnsholt

Rapid single-nucleotide polymorphism-based identification of clonal Pseudomonas aeruginosa isolates from patients with cystic fibrosis by the use of real-time.

E. Galmozzi, F. Facchetti, R. Perbellini, A. Aghemo

Rapid detection of the recently emerged Bordetella pertussis strains with the ptxP3 pertussis toxin promoter allele by real-time PCR T. Kallonen, J.

Development of a real-time fluorescence resonance energy transfer PCR to identify the main pathogenic Campylobacter spp. A. Ménard, F. Dachet, V. Prouzet-Mauleon,

Clinical Microbiology and Infection

Vector control: a cornerstone in the malaria elimination campaign

A comparison of three real-time PCR assays for the confirmation of Neisseria gonorrhoeae following detection of N. gonorrhoeae using Roche COBAS AMPLICOR

Detection of human metapneumovirus and respiratory syncytial virus by duplex real-time RT-PCR assay in comparison with direct fluorescent assay P. Jokela,

Development and evaluation of a multiplex test for the detection of atypical bacterial DNA in community-acquired pneumonia during childhood J.A. Carrillo,

Clinical microbiologists facing an anthrax alert

PCR amplification and high-resolution melting curve analysis as a rapid diagnostic method for genotyping members of the Mycobacterium avium–intracellulare.

Metagenomics and probiotics

M. Ylihärsilä, E. Harju, R. Arppe, L. Hattara, J. Hölsä, P

R. Alonso, C. Muñoz, T. Peláez, E. Cercenado, M. Rodríguez-Creixems, E

T.M. File Clinical Microbiology and Infection

Update on antifungal resistance in Aspergillus and Candida

Survival of hepatitis A and E viruses in soil samples

Nosocomial listeria gastroenteritis in a newborn, confirmed by random amplification of polymorphic DNA H. Hof, R. Lampidis, J. Bensch Clinical Microbiology.

A novel fluorescence in situ hybridization test for rapid pathogen identification in positive blood cultures A. Makristathis, S. Riss, A.M. Hirschl

Real-time PCR assays compared to culture-based approaches for identification of aerobic bacteria in chronic wounds J.H. Melendez, Y.M. Frankel, A.T.

R. Slinger, I. Moldovan, N. Barrowman, F. Chan

Twelve years of fluconazole in clinical practice: global trends in species distribution and fluconazole susceptibility of bloodstream isolates of Candida

A.P. Underwood, J. Green Clinical Microbiology and Infection

H. Leblebicioglu, C. Eroglu Clinical Microbiology and Infection

Rapid identification of fungal pathogens in positive blood cultures using oligonucleotide array hybridization H-C. Hsiue, Y-T. Huang, Y-L. Kuo, C-H.

An after-hours clinical liaison blood culture service—is it worth it?

S. Alexiou-Daniel, A. Stylianakis, A. Papoutsi, I. Zorbas, A. Papa, A

High-resolution melting analysis for the detection of two erythromycin-resistant Bordetella pertussis strains carried by healthy schoolchildren in China

Detection of Bartonella henselae and Bartonella quintana by a simple and rapid procedure using broad-range PCR amplification and direct single-strand.

Usefulness of a quantitative real-time PCR assay using serum samples to discriminate between inactive, serologically positive and active human brucellosis

G.C. Schito Clinical Microbiology and Infection

Three years experience of real-time PCR for the diagnosis of Q fever

Impact of antibiotic restrictions: the patient's perspective

The future of diagnostic bacteriology

Presentation transcript:

Evaluation of a single-tube real-time PCR for detection and identification of 11 dermatophyte species in clinical material A.M.C. Bergmans, M. van der Ent, A. Klaassen, N. Böhm, G.I. Andriesse, R.G.F. Wintermans Clinical Microbiology and Infection Volume 16, Issue 6, Pages 704-710 (June 2010) DOI: 10.1111/j.1469-0691.2009.02991.x Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Multiple sequence alignments of partial internal transcribed sequence 1 regions from all dermatophyte species detected by the LightCycler real-time PCR assay. Nucleotides corresponding to the species-specific hybridization probe sets are bold and underlined. Clinical Microbiology and Infection 2010 16, 704-710DOI: (10.1111/j.1469-0691.2009.02991.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 (a) Typical melting curve analysis result in channel 670 of the LightCycler 2.0, after real-time PCR amplification of Trichophyton interdigitale, Trichophyton mentagrophytes, and Trichophyton tonsurans (DNA from cultured isolates spiked into DNA from negative clinical samples), using the species-specific hybridization probe set Tinmeto1S + Tinmeto3A. The values on the y-axis are the first negative derivative of the change in fluorescence (dFluorescence (670/back530)) divided by the change in temperature (dT). The three vertical lines show the melting temperatures (Tm) of the three dermatophyte species with the probe set. (b) Melting curve analysis result in channel 670 from clinical samples containing T. tonsurans (Tm c. 58°C) or T. interdigitale (Tm c. 65°C). Clinical Microbiology and Infection 2010 16, 704-710DOI: (10.1111/j.1469-0691.2009.02991.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions