Fig. 4. miR-370 restoration suppressed EMT progression in HCC cells

Slides:



Advertisements
Similar presentations
Fig. 3. Effect of tolfenamic acid (TA) on the MAPK pathway in nasopharyngeal cancer cells. (A) Immunoblot of TA-treated cells stained with antibodies against.
Advertisements

C/EBPα inhibits hepatocellular carcinoma by reducing Notch3/Hes1/p27 cascades  Yi-Chao Shi, Hong Zhao, Chuan Yin, Xin Zeng, Qing Zhang, Wen-Ping Xu, Ji.
Astragaloside IV Enhances Cisplatin Chemosensitivity in Non-Small Cell Lung Cancer Cells Through Inhibition of B7-H3 Cell Physiol Biochem 2016;40:
From: Regulation of ADAM10 and ADAM17 by Sorafenib Inhibits Epithelial-to-Mesenchymal Transition in Epstein-Barr Virus–Infected Retinal Pigment Epithelial.
Downregulation of MicroRNA-145 Caused by Hepatitis B Virus X Protein Promotes Expression of CUL5 and Contributes to Pathogenesis of Hepatitis B Virus-Associated.
Volume 355, Issue 2, Pages (December 2014)
WNT5A is a Key Regulator of the Epithelial-Mesenchymal Transition and Cancer Stem Cell Properties in Human Gastric Carcinoma Cells Pathobiology 2013;80:
MicroRNA-101 Inhibits Growth, Proliferation and Migration and Induces Apoptosis of Breast Cancer Cells by Targeting Sex-Determining Region Y-Box 2 Cell.
From: Inhibition of miR-205 Impairs the Wound-Healing Process in Human Corneal Epithelial Cells by Targeting KIR4.1 (KCNJ10) Invest. Ophthalmol. Vis. Sci..
Med Princ Pract 2016;25(suppl 2): DOI: /
Molecular Therapy - Oncolytics
From: β-Catenin/CBP–Dependent Signaling Regulates TGF-β–Induced Epithelial to Mesenchymal Transition of Lens Epithelial Cells Invest. Ophthalmol. Vis.
Cell Physiol Biochem 2017;44:1867– DOI: /
Elevated FOXC2 Expression Promotes Invasion of HCC Cell Lines and is Associated with Poor Prognosis in Hepatocellular Carcinoma Cell Physiol Biochem 2017;44:99–109.
Volume 139, Issue 6, Pages e12 (December 2010)
A B C MDA-MB-231 OHT: h FOXM1 PLK CDC25b ERα Tubulin
Supplementary FigureS4 (Feduska et al.)
Cell Physiol Biochem 2015;36: DOI: /
Long Noncoding RNA HOTAIR Controls Cell Cycle by Functioning as a Competing Endogenous RNA in Esophageal Squamous Cell Carcinoma  Kewei Ren, Yahua Li,
a b SUM149PT Motility Invasion C V C V
Andrea L Kasinski, Frank J Slack  Molecular Therapy - Nucleic Acids 
Snail Enhances Glycolysis in the Epithelial-Mesenchymal Transition Process by Targeting FBP1 in Gastric Cancer Cell Physiol Biochem 2017;43:31–38 - DOI: /
Up-Regulation of RFC3 Promotes Triple Negative Breast Cancer Metastasis and is Associated With Poor Prognosis Via EMT  Zhen-Yu He, San-Gang Wu, Fang Peng,
SDHB deficiency promotes TGFβ-mediated invasion and metastasis of colorectal cancer through transcriptional repression complex SNAIL1-SMAD3/4  Haiyu Wang,
Inhibition of ILK restores epithelial ZO-1 and E-cadherin and inhibits fibronectin and Snail1 expression after TGF-β1 treatment. Inhibition of ILK restores.
MicroRNA-489 Plays an Anti-Metastatic Role in Human Hepatocellular Carcinoma by Targeting Matrix Metalloproteinase-7  Yixiong Lin, Jianjun Liu, Yuqi Huang,
Inhibition of ILK by QLT-0267 abrogates PAI-1 and MMP-2 induction by TGF-β1 in tubular epithelial cells. Inhibition of ILK by QLT-0267 abrogates PAI-1.
Chandra M.V. Goparaju, PhD  Journal of Thoracic Oncology 
GM-CSF induces tumor cell EMT and stemness.
Acriflavine Inhibits Acquired Drug Resistance by Blocking the Epithelial-to-Mesenchymal Transition and the Unfolded Protein Response  Jeroen Dekervel,
Volume 139, Issue 6, Pages e12 (December 2010)
Epithelial-Mesenchymal Transitions
Reduction of Sca-1 increases PPARγ expression.
Figure 5 The mechanism underlying epithelial-to-mesenchymal
Volume 143, Issue 6, Pages e5 (December 2012)
Cadherin 11, a miR-675 Target, Induces N-Cadherin Expression and Epithelial– Mesenchymal Transition in Melasma  Nan-Hyung Kim, Soo-Hyun Choi, Tae Ryong.
Volume 15, Issue 6, Pages (May 2016)
Volume 19, Issue 2, Pages (February 2011)
Estrogen Upregulates Slug to Enhance the Migration of Keratinocytes
Molecular Therapy - Nucleic Acids
Volume 19, Issue 4, Pages (April 2017)
Maxime Janin, Manel Esteller  Cell Metabolism 
Fig. 7. Altering GUCD1 expression reversed the effect of miR-370 on cell EMT process in HCC. (A) Western blot and (B) qRT-PCR results showed that GUCD1overexpression.
Volume 54, Issue 5, Pages (May 2011)
Fig. 3. miR-370 overexpression suppressed cell migration and invasion in HCC cells. (A) miR-370 expression in miR-370 overexpressed HCCLM3 cells were detected.
Fig. 1. miR-370 was downregulated, and GUCD1 was upregulated in HCC
Fig. 5. GUCD1 was a direct target of miR-370 in HCC
Fig. 6. Alteration of GUCD1 expression partially reversed the miR-370-mediated effect on HCC cell migration and invasion. (A) Western blot (up) and qRT-PCR.
A B C D eIF3e shRNA 205 clones Control shRNA 3 7 eIF3e b-actin
XIST regulated miR-29c by directly targetting in TMZ-resistant glioma cells XIST regulated miR-29c by directly targetting in TMZ-resistant glioma cells.
Overexpression of HGF decreases MET protein levels.
Volume 16, Issue 1, Pages (January 2015)
LncRNA TRERNA1 Function as an Enhancer of SNAI1 Promotes Gastric Cancer Metastasis by Regulating Epithelial-Mesenchymal Transition  Huazhang Wu, Ying.
Volume 25, Issue 3, Pages (March 2017)
A and B, CO-1686–resistant NCI-H1975 cell clones, COR1-1 and COR10-1 display a reduced dependence on EGFR signaling for survival. A and B, CO-1686–resistant.
Volume 65, Issue 4, Pages (October 2016)
USP2a-dependent miRs deregulation modulates MYC expression.
Epithelial to Mesenchymal Transition in an Epidermal Growth Factor Receptor-Mutant Lung Cancer Cell Line with Acquired Resistance to Erlotinib  Kenichi.
Fig. 4. Downregulation of KDM4C restores the integrity of DNA mismatch repair.(A) An outline describing the experimental flow to assess the effect of KDM4C.
PTK6 downregulation suppresses SNAIL and increases E-cadherin expression. PTK6 downregulation suppresses SNAIL and increases E-cadherin expression. A,
(fold of TGF-β1 response)
Targeting eIF4E increases ZEB1 protein and mRNA levels and decreases ZEB1-targeting miR-200c and miR-141 microRNAs. Targeting eIF4E increases ZEB1 protein.
a b 10A.zp85WT 10A.zp85S83μ 10A.vec 10A.z 10A.vec 10Az 10Az.p85WT
Molecular Therapy - Oncolytics
Parenchymal, nonparenchymal, and immune cells of the liver and their role in metastasis. Parenchymal, nonparenchymal, and immune cells of the liver and.
Expression of CRC stem cell markers and L1 in CRC cells.
FGFR1 is dominantly expressed in mesenchymal-like KRAS-mutant lung cancer cell lines. FGFR1 is dominantly expressed in mesenchymal-like KRAS-mutant lung.
Cell-cycle regulatory proteins were controlled by O-GlcNAc at FOXO3 S284 through MDM2. Cell-cycle regulatory proteins were controlled by O-GlcNAc at FOXO3.
PARP1 suppresses the transcription of PD-L1 in cancer cells.
Molecular Therapy - Nucleic Acids
Presentation transcript:

Fig. 4. miR-370 restoration suppressed EMT progression in HCC cells Fig. 4. miR-370 restoration suppressed EMT progression in HCC cells. (A) Western blot analysis and (B) qRT-PCR results of miR- 370 overexpression in EMT of HCCLM3 cells. (C) Western blot and (D) qRT-PCR results indicated that inhibition of miR-370 in Hep3B cells downregulated E-cadherin expression and enhanced N-cadherin and Vimentin expression. *p<0.05, †p<0.01. EMT, epithelial-mesenchymal transition; HCC, hepatocellular carcinoma. Yonsei Med J. 2019 Mar;60(3):267-276. https://doi.org/10.3349/ymj.2019.60.3.267