TPCS Product List Hepatocyts.

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TPCS Product List Hepatocyts

Hepatocytes Product Name Cat. No. Product Format Size C57BL6 Mouse Hepatocytes HEP004MC-96P Fresh 96-Well Plate (0.03M per well) HEP004MC-12P 12-Well Plate (0.4M per well) SD Rat Hepatocytes HEP005MSD-96P 96-Well Plate (0.04M per well) HEP005MSD-12P 12-Well Plate (0.5M per well)

Metabolite Rate Analysis of metabolite rate with test substrate (formation is measured in nmol/h/million cells ) using Hepatocytes isolated from C57BL/6. Substrate Metabolite Enzyme Historical data Phenacetin Acetaminophen CYP1A 5nmole/h/million cells; 3nmole/h/million cells; 4nmole/h/million cells; 8nmole/h/million cells; 9nmole/h/million cells; 2nmole/h/million cells; 14nmole/h/million cells; 20nmole/h/million cells; Hepatocytes were incubated in suspension at 37°C , 5% CO2 in culture medium with the test substrates for 1 hour. Production of metabolites was measured by LC-MS/MS. Activities are expressed as nanomole of metabolite formed/h/million cells, which is equivalent to nmole/hr/mg of protein. To convert to pmole of metabolite/min/million hepatocytes multiply the results by 16.66.