Satish Pradhan Dnyanasadhana College, Thane. Department of Chemistry S Satish Pradhan Dnyanasadhana College, Thane. Department of Chemistry S.Y.B.Sc. Analytical Chemistry Sem-IV Performance Characteristics of an Analytical method Dr.G.R.Bhagure

Limit of quantification, Performance Characteristics of an Analytical method Accuracy, Precision, Sensitivity, Specificity, Selectivity, Robustness, Ruggedness, Linearity range, Limit of quantification, Limit of Detection, Signal to Noise ratio.

3.1.3 Performance Characteristics of an Analytical method Accuracy, Precision, Sensitivity, Specificity, Selectivity, Robustness, Ruggedness, Linearity range, Limit of quantification, Limit of Detection, Signal to Noise ratio.

Performance Characteristics of an Analytical method The criteria that are used to judge a technique and more often applied to applied to compare two different analytical method

Performance characteristics of analytical Method : 1 Accuracy: It is defined as closeness of the observed value with the true value. 2 Precision: It is the agreement between Individual observations of same set. 3 Limit of detection(LOD): Minimum amount of concentration of a component that can be detected with a given degree of confidence. 4 Limit of Quantification (LOQ): Minimum amount of concentration of a component that can be estimated with a given degree of confidence is termed as LOQ.

Precision and Accuracy Measurements that are close to the “correct” value are accurate. Measurements which are close to each other are precise. Measurements can be accurate and precise; precise but inaccurate; neither accurate nor precise.

Performance characteristics of analytical Method : 5 Limit of Linearity (LOL) : It is defined as maximum concentration range up to which instrument is produces linear response. 6 Sensitivity: It is a measure of ability of method to discriminate between two small concentration differences in the analyte. 7 Measurement of Sensitivity: It is measured in terms of slope of the calibration curve. If the slope is greater sensitivity of the method is high and vise-versa.

Performance characteristics of analytical Method : 8 Selectivity: It is defined as degree to which the method is free from interferences from other components present in the matrix. OR An analytical method is selective if its signal is a function of only the amount of analyte present in the sample. In the presence of an interferent, 9 Dynamic range: It is the concentration range from limit of Quantification (LOQ) to Limit of Linearity (LOL).

Performance characteristics of analytical Method : 10 Selectivity: It is defined as degree to which the method is free from interferences from other components present in the matrix. 11 Dynamic range: It is the concentration range from limit of Quantification (LOQ) to Limit of Linearity (LOL).

Performance characteristics of analytical Method : 12 Robustness: When a method is relatively free from chemical interferences, it can be applied to the determination of analyte's in a wide variety of sample matrices. Such methods are considered robust. 13 Ruggedness:It is the degree of reproducibility of test results of an analytical methods obtained by the analysis of same sample under variety of conditions. 14 Signal to Noise ratio: The ratio of the signal’s intensity to the average intensity of the surrounding noise.

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