Changes in gene expression level of 40 genes of MG1655 on exposure to GSH and/or ciprofloxacin by RT-qPCR analysis. Changes in gene expression level of.

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Changes in gene expression level of 40 genes of MG1655 on exposure to GSH and/or ciprofloxacin by RT-qPCR analysis. Changes in gene expression level of 40 genes of MG1655 on exposure to GSH and/or ciprofloxacin by RT-qPCR analysis. The 40 genes are the same as those which exhibited ≥5.0-fold-altered expression in microarray analysis. Mid-logarithmic-phase bacterial cells were used for RT-qPCR analysis per the details mentioned in Materials and Methods. Data represent expression levels of different genes in the presence of (A) 10 mM GSH, (B) 3 ng of ciprofloxacin/ml, and (C) 10 mM GSH with 30 ng of ciprofloxacin/ml. Each bar represents the ratio of the expression level of that gene in the presence of the given exposure condition to the expression level under the control conditions using mdoG as an external reference gene. The bar diagram shown here represents data from three different biological replicates (n = 3). Error bars represent ± standard errors of the means (SEM). Manish Goswami, and Akkipeddi Venkat Satya Surya Narayana Rao mSystems 2018; doi:10.1128/mSystems.00001-18