1- Molecular weight marker 2- BamHI 3- BamHI 4 –EcoRI 5 – Hind III

Slides:

Advertisements

Similar presentations

Molecular Biology Working with DNA. Topics  Genomic vs. Vector DNA  Purifying plasmid DNA  Restriction enzymes  Restriction maps.

Advertisements

 94 degrees C 30 seconds  64 degrees C 30 seconds  72 degrees C 1 minute  After 25 cycles finish with 72 degrees C for 5 minutes Novablue cells were.

Restriction Mapping.

Goal: To identify yeast gene products important for accurate chromosome transmission in mitosis. Importance: Errors during chromosome transmission in humans.

Gel Electrophoresis Lab

In what phase is cell #1?. Name this part of the microscope.

DNA Fingerprinting. Use of DNA to Determine Identity DNA controls production of proteins DNA controls production of proteins Results in phenotype (eye.

$100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300 $400 $500 $100 $200 $300.

What are Plasmids? Plasmids are circular pieces of bacterial DNA that often contain genes not related to basic life functions Often contain antibiotic.

SIGNS, SIGNALS AND ROADWAY MARKINGS. SECTION OBJECTIVES 1. State the meaning of the eight shapes and colors used for traffic signs 2. Describe the actions.

40 Minutes Left.

A 2 nd year college student working in the lab for the summer forgot to label the Tube of plasmid DNA that he/she was given. So being the brilliant graduate.

Signals, pavement markings, and proper turns QUIZ

Midterm Breakdown Part I Part II: Part III : 8 calculations

Volume 14, Issue 3, Pages (March 2007)

DNA analysis on patients with STS deficiency

Restriction Enzymes and Plasmid Mapping

RE Digestion Field Trip Results

ФОНД ЗА РАЗВОЈ РЕПУБЛИКЕ СРБИЈЕ

Primers 2 primers are required for exponential amplification

by Jian-Min Wang, Hong Zheng, Mila Blaivas, and Kotoku Kurachi

What are Plasmids? Plasmids are circular pieces of bacterial DNA that often contain genes not related to basic life functions Often contain antibiotic.

Skin-Specific Expression of ank-393, a Novel Ankyrin-3 Splice Variant

الفعل ورد الفعل ♠ ♠ ♠ مجلس أبوظبي للتعليم منطقة العين التعليمية

Analysis of T-Cell Clonality Using Laser Capture Microdissection and High-Resolution Microcapillary Electrophoresis Evgeny Yakirevich, Cynthia L. Jackson,

Isothermal Multiple Displacement Amplification

The Orientation of Mycobacteriophage Bxb1 Integration Is Solely Dependent on the Central Dinucleotide of attP and attB Pallavi Ghosh, Amy I Kim, Graham.

Molecular Biology Restriction enzymes.

by Milind C. Mahajan, and Sherman M. Weissman

15 seconds left 30 seconds left 3 minutes left 2 minutes left 1 minute

Silencing in Yeast rDNA Chromatin

Modulation of the expression of tissue plasminogen activator and its inhibitor by hypoxia in human peritoneal and adhesion fibroblasts Ghassan M Saed,

'III \-\- I ', I ,, - -

Volume 7, Issue 4, Pages (October 1997)

RE Digestion Field Trip Results

Short Telomeres in Yeast Are Highly Recombinogenic

What number is the arrow pointing at?

Molecular Biology Restriction enzymes.

Mass and Molar Ratios of DNA

Targeting AMPK in prostate cancerMT 63–78 (green burst) binds to the β1 subunit of AMPK, activating its enzymatic function. Targeting AMPK in prostate.

Jung-Ok Han, Sharri B Steen, David B Roth Molecular Cell

Products seen by gel electrophoresis following two rounds of the multiplex herpesvirus PCR. The material in lanes 1–15 was derived from genital swabs.

A, Western blot analysis of fetuin-A in AGA (lanes 1–5 and 10–13) and IUGR (lanes 6–9, 14, and 15) UC plasma samples. A, Western blot analysis of fetuin-A.

Volume 119, Issue 4, Pages (October 2000)

Molecular Biotechnology

Bio-Rad Biotechnology Explorer™ DNA Fingerprinting Kit

Localization of selected clones in mammalian COS-7 cells.

RNA-Independent DNA Cleavage Activities of Cas9 and Cas12a

Propagation of Dachsous-Fat Planar Cell Polarity

Frpo: A Novel Single-Stranded DNA Promoter for Transcription and for Primer RNA Synthesis of DNA Replication Hisao Masai, Ken-ichi Arai Cell Volume.

Coding Joint Formation in a Cell-Free V(D)J Recombination System

1- Molecular weight marker 2- BamHI 3- BamHI 4 –EcoRI 5 – Hind III

Expanding the Range of CRISPR/Cas9 Genome Editing in Rice

Systemic allergic reaction to coconut (Cocos nucifera) in 2 subjects with hypersensitivity to tree nut and demonstration of cross-reactivity to legumin-like.

Ruth Halaban, Elaine Cheng Journal of Investigative Dermatology

Volume 8, Issue 1, Pages (January 1998)

DNA Technology Review.

Gabriella Esposito, Giuseppe Rescigno, Francesco Salvatore

Retrospective tissue typing of the kidney donor from recipient urine

Volume 14, Issue 3, Pages (March 2007)

Molecular Biotechnology 4C x MWM

Molecular Therapy - Methods & Clinical Development

Biochemical characterization and localization of EGFRΔ768.

Antibody staining of SuUR protein.

Purified Vpr induces double-strand breaks in vitro.

Volume 103, Issue 5, Pages (November 2000)

Forensic DNA Fingerprinting: Using Restriction Enzymes

Presentation transcript:

1- Molecular weight marker 2- BamHI 3- BamHI 4 –EcoRI 5 – Hind III Mol Biotec Class 4B 7-12-2016 1 2 3 4 5 6 7 1- Molecular weight marker 2- BamHI 3- BamHI 4 –EcoRI 5 – Hind III 6- PstI 7- PstI The membrane is relatively dark, as I left the enzymatic reaction for ~20 minutes to see if any bands would became visible. There is a faint band (red arrow) in lanes 3 – Bam HI and 7 -PstI. As in the other lanes there is no signal, use the reference membrane for the analysis and discussion of results.