Splenic CD169+ macrophages express a unique gene profile.

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Splenic CD169+ macrophages express a unique gene profile. Splenic CD169+ macrophages express a unique gene profile. (A) PCA of 17,336 genes using each sample’s log2[fragments per kilobase of transcript per million mapped reads (FPKM) + 1]. (B) Heat map using z scores for 709 differentially expressed genes between naïve CD169+ macrophages and red pulp macrophages (F4/80+). (C) Transcript expression (FPKM) of paired-end sample sequencing represented as normalized values based on transcript length and depth of coverage. Genes represent transcripts that were previously identified to distinguish red pulp macrophages from other subsets of tissue macrophages. Data are shown as means ± SEM (*P < 0.05 and **P < 0.005, Student’s t test). ns, not significant. (D) Heat map using z scores for the identified 795 differentially expressed genes comparing CD169+ macrophages from naïve and Lm-infected mice. (E) Transcript expression (FPKM) of genes exclusively up-regulated in CD169+ macrophages 12 hours after Lm infection. Data are shown as means ± SEM (*P < 0.05 and **P < 0.005, Student’s t test). Oriana A. Perez et al. Sci. Immunol. 2017;2:eaah5520 Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works