Relationship between human semen parameters and deoxyribonucleic acid damage assessed by the neutral comet assay Ana T. Trisini, B.S., Narendra P. Singh,

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Presentation transcript:

Relationship between human semen parameters and deoxyribonucleic acid damage assessed by the neutral comet assay Ana T. Trisini, B.S., Narendra P. Singh, M.B.B.S., M.S., Susan M. Duty, M.S.N., Sc.D., Russ Hauser, M.D., Sc.D., M.P.H. Fertility and Sterility Volume 82, Issue 6, Pages 1623-1632 (December 2004) DOI: 10.1016/j.fertnstert.2004.05.087 Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions

FIGURE 1 (A) Cell with a short comet tail. (B) Considerable heterogeneity of comet tail lengths within an individual. (C) Cell with a long comet tail. (D) Comet referred to as a cell with high DNA damage (CHD), too long to measure with the image analysis software. Scale bars, 50 μm. Fertility and Sterility 2004 82, 1623-1632DOI: (10.1016/j.fertnstert.2004.05.087) Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions

FIGURE 2 Median (25th, 75th percentiles) for comet assay parameters, plotted by semen parameters dichotomized according to WHO reference valves. TDM = tail distributed moment; CHD = cells with highly damaged DNA. Fertility and Sterility 2004 82, 1623-1632DOI: (10.1016/j.fertnstert.2004.05.087) Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions