Easy sperm processing technique allowing exclusive accumulation and later usage of DNA-strandbreak-free spermatozoa T. Ebner, O. Shebl, M. Moser, R.B.

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Presentation transcript:

Easy sperm processing technique allowing exclusive accumulation and later usage of DNA-strandbreak-free spermatozoa T. Ebner, O. Shebl, M. Moser, R.B. Mayer, W. Arzt, G. Tews Reproductive BioMedicine Online Volume 22, Issue 1, Pages 37-43 (January 2011) DOI: 10.1016/j.rbmo.2010.09.004 Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Schematic cross-section of the sperm selector indicating the ejaculate in the outer ring (dots) and medium in the centre well (wave lines). Cover glass is not shown. (a) Sperm selector consists of the actual chamber (glass or polyethylene) and a U-ring (top left). (b) After filling of the chamber, the U-ring is inserted and creates a capillary bridge allowing motile spermatozoa to swim to the centre well (theoretical path of migration indicated by arrow). Reproductive BioMedicine Online 2011 22, 37-43DOI: (10.1016/j.rbmo.2010.09.004) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 The Zech-selector. The outer diameter of the glass chamber is 6.3cm. The diameter of the inner circle, considered for concentration of motile spermatozoa, is 2cm. Reproductive BioMedicine Online 2011 22, 37-43DOI: (10.1016/j.rbmo.2010.09.004) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Sperm chromatin dispersion (SCD) test in a patient showing approximately 30% strandbreak-positive spermatozoa in the raw semen. Arrow heads indicate spermatozoa with no or minor halos (i.e. those with strand breaks). Bar=50μm. Reproductive BioMedicine Online 2011 22, 37-43DOI: (10.1016/j.rbmo.2010.09.004) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions