Size profile of viral small RNAs obtained from control cells and RIG-I- and MDA5-deficient (KO) cells after infection for 24 h with SINV at an MOI of 0.1, for 48 h with YFV17D at an MOI of 0.1, or for 16 h with CBV3 at an MOI of 0.01. Size profile of viral small RNAs obtained from control cells and RIG-I- and MDA5-deficient (KO) cells after infection for 24 h with SINV at an MOI of 0.1, for 48 h with YFV17D at an MOI of 0.1, or for 16 h with CBV3 at an MOI of 0.01. Small RNAs were mapped to the sense (blue bars) and antisense (red bars) strands of the viral genome, allowing 1 mismatch. Sense and antisense reads are presented on different scales, as antisense reads were very scarce. RPM, reads per million. Susan Schuster et al. mSphere 2017; doi:10.1128/mSphere.00333-17