David D. Tieu, MD, Anju T. Peters, MD, Roderick T

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Presentation transcript:

Evidence for diminished levels of epithelial psoriasin and calprotectin in chronic rhinosinusitis David D. Tieu, MD, Anju T. Peters, MD, Roderick T. Carter, BS, Lydia Suh, BS, David B. Conley, MD, Rakesh Chandra, MD, James Norton, MS, Leslie C. Grammer, MD, Kathleen E. Harris, BS, Atsushi Kato, PhD, Robert C. Kern, MD, Robert P. Schleimer, PhD Journal of Allergy and Clinical Immunology Volume 125, Issue 3, Pages 667-675 (March 2010) DOI: 10.1016/j.jaci.2009.11.045 Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Evaluation of expression of S100A7 by means of ELISA in sinonasal tissue extracts. A, Expression of S100A7 protein is increased in inferior turbinate tissue (IT) compared with that seen in uncinate tissue (UT) from healthy subjects. The statistical bars indicate a significant difference among the corresponding groups. B, Levels of S100A7 were increased in all CRS tissue extracts when compared with those in extracts of uncinate tissue from control subjects. The statistical bars indicate a significant difference among the corresponding groups. A P value of less than .05 is considered statistically significant. Journal of Allergy and Clinical Immunology 2010 125, 667-675DOI: (10.1016/j.jaci.2009.11.045) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Evaluation of expression of human calprotectin (S100A8/A9) and human neutrophil elastase by means of ELISA in sinonasal tissue extracts. A, Human S100A8/A9 protein levels in tissue extracts normalized to total protein. Polyp tissue from patients with CRSwNP demonstrates a significant increase in human calprotectin levels when compared with normal tissue extracts (P < .01). B, Linear regression analysis of matching S100A8/A9 and human neutrophil elastase samples demonstrates a significant correlation between the 2 proteins (P < .0001, r = 0.6577). C, Human neutrophil elastase protein levels in polyp tissue from patients with CRSwNP are significantly increased when compared with those seen in normal tissue extracts (P < .05). IT, Inferior turbinate tissue; UT, uncinate tissue. Journal of Allergy and Clinical Immunology 2010 125, 667-675DOI: (10.1016/j.jaci.2009.11.045) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Expression of S100A7 and S100A8/A9 in nasal lavage fluids and serum samples, as determined by means of ELISA. A and B, Human S100A7 (∗P < .01, ∗∗P < 0.001; Fig 3, A) and human S100A8/A9 (Fig 3, B) protein levels in nasal lavage fluid. Human S100A7 protein levels are significantly decreased in nasal lavage specimens of patients with CRS and AR when compared with those seen in control subjects (P < .05). Human S100A8/A9 protein levels are significantly decreased in nasal lavage specimens of patients with CRSwNP when compared with those seen in control subjects. C and D, Human S100A7 (P > .05; Fig 3, C) and human S100A8/A9 (P < .05; Fig 3, D) protein levels in serum. Only human S100A8/A9 protein levels are significantly decreased in patients with CRSwNP when compared with those seen in control subjects. A P value of less than .05 is considered statistically significant. Journal of Allergy and Clinical Immunology 2010 125, 667-675DOI: (10.1016/j.jaci.2009.11.045) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Immunohistochemical staining for S100A7 in representative tissue samples from the uncinate process and nasal polyps (original magnification ×400). A, Negative control of uncinate from a representative control subject did not stain. B-D, S100A7 staining of uncinate from a control subject (Fig 4, B) showed intense staining in the epithelial and glandular tissue, whereas light-to-moderate staining of S100A7 was seen in uncinate samples from a patient with CRSsNP (Fig 4, C) and a patient with CRSwNP (Fig 4, D). E, Minimal S100A7 staining in epithelial cells was seen in polyp tissue. F, Intense glandular staining for S100A7 in the uncinate tissue of a control subject. G and H, Less staining was seen in the uncinate tissue from a patient with CRSsNP (Fig 4, G) and a patient with CRSwNP (Fig 4, H). Original magnification was 400×. Journal of Allergy and Clinical Immunology 2010 125, 667-675DOI: (10.1016/j.jaci.2009.11.045) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 Immunohistochemical staining for S100A8/A9 in representative tissue samples from the uncinate process and nasal polyps (original magnification ×400). A, Negative control of uncinate tissue from a control subject did not stain. B, S100A8/A9 staining of uncinate tissue from a control subject showed moderate staining in the epithelial and glandular tissue. Also note several intensely staining leukocytes that we believe to be neutrophils (Fig 5, D). C and D, Some light staining of S100A8/A9 was seen in uncinate samples from a patient with CRSsNP (Fig 5, C) and a patient with CRSwNP (Fig 5, D). E, Minimal S100A8/A9 staining in epithelial cells was seen in polyp tissue. F, Glandular staining in the uncinate tissue of a control subject with moderate staining for S100A8/A9. G and H, Diffuse staining was seen in the uncinate tissue from a patient with CRSsNP (Fig 5, G) and a patient with CRSwNP (Fig 5, H). Original magnification was 400×. Journal of Allergy and Clinical Immunology 2010 125, 667-675DOI: (10.1016/j.jaci.2009.11.045) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions