Volume 61, Issue 1, Pages (January 2002)

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Volume 61, Issue 1, Pages 186-194 (January 2002) Oxidative stress and nitric oxide synthase in rat diabetic nephropathy: Effects of ACEI and ARB Maristela Lika Onozato, Akihiro Tojo, M.D., Atsuo Goto, Toshiro Fujita, Christopher S. Wilcox Kidney International Volume 61, Issue 1, Pages 186-194 (January 2002) DOI: 10.1046/j.1523-1755.2002.00123.x Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 1 Light micrographs illustrating immunostaining for NAD(P)H oxidase p47phox in glomerulus (a, c, e, g) and inner medulla (b, d, f, h). (a, b) control rat; (c, d) diabetic rat; (e, f) diabetic treated with quinapril; (g, h) diabetic treated with candesartan. Magnification ×280. Kidney International 2002 61, 186-194DOI: (10.1046/j.1523-1755.2002.00123.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 2 Western blot for p47phox using whole kidney homogenate analyzed by densitometry (mean ± SEM from 6 rats in each group). A prominent band for p47phox is observed at 47 kD. Abbreviations are: DM, streptozotocin-induced diabetic rats; DM + QUI, diabetic rats given quinapril; DM + CAN, diabetic rats given candesartan. *P < 0.0005 vs. Control; +P < 0.005, ++P < 0.002 vs. DM. Kidney International 2002 61, 186-194DOI: (10.1046/j.1523-1755.2002.00123.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 3 Light micrographs illustrating immunostaining for neuronal nitric oxide synthase (nNOS) and endothelial nitric oxide synthase (eNOS). Immunoreactivity for nNOS is observed in macula densa and that for eNOS is in endothelial cells in glomerulus and renal artery. Magnification ×280 for nNOS, ×240 for eNOS in glomerulus and ×180 for eNOS in renal artery. Kidney International 2002 61, 186-194DOI: (10.1046/j.1523-1755.2002.00123.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 4 Western blot studies of nNOS expression in the whole kidney homogenates showing a specific band at 160 kD. The bands were quantified by densitometry and expressed as mean ± SEM values from six rats in each group. Kidney International 2002 61, 186-194DOI: (10.1046/j.1523-1755.2002.00123.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 5 Western blot of eNOS expression in the whole kidney homogenates were quantified by densitometry (mean ± SEM from 6 rats in each group). A specific band for eNOS was observed at 133 kD. *P < 0.05 vs. Control; +P < 0.001 vs. DM. Kidney International 2002 61, 186-194DOI: (10.1046/j.1523-1755.2002.00123.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 6 Western blot of nitrotyrosine in whole kidney homogenates quantified by densitometry (mean ± SEM from 6 rats in each group). There was a proximate band at 60 kD. *P < 0.05 vs. Control; +P < 0.05, ++P < 0.01 vs. DM. Kidney International 2002 61, 186-194DOI: (10.1046/j.1523-1755.2002.00123.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 7 Albumin excretion in control rats and over four weeks of diabetes mellitus alone or with two weeks of quinapril or candesartan administration (weeks 2–4). Symbols are: (▪) Control; (•) DM; (▵) DM + QUI; (⋄) DM + CAN; *P < 0.005 vs. Control; +P < 0.05, ++P < 0.02 vs. DM. Kidney International 2002 61, 186-194DOI: (10.1046/j.1523-1755.2002.00123.x) Copyright © 2002 International Society of Nephrology Terms and Conditions