Expression of CD9 in frozen–thawed mouse oocytes: preliminary experience  Yan Wen, M.D., Rudy Quintero, M.D., Bertha Chen, M.D., Yimin Shu, Ph.D., Mary Lake Polan, M.D., Ph.D., Barry Behr, Ph.D.  Fertility and Sterility  Volume 88, Issue 2, Pages 526-529 (August 2007) DOI: 10.1016/j.fertnstert.2006.11.130 Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

FIGURE 1 (A) CD9 mRNA expression levels in mouse oocytes were determined by relative quantitative real time RT-PCR. Values of the CD9 gene were normalized using H2A.Z to one normalized control sample (calibrator, ovarian cDNA), which was used to generate the relative quantification to calibrator (Rel.Quant. to Cal.). Bars, mean ± SE of Rel.Quant. to Cal from 38 frozen–thawed (0.172 ± 0.081) and 40 fresh oocytes (12.47 ± 2.49). *P<.0001. (B) Western blotting analysis of CD9 protein expression in oocytes. Bars, mean ± SE of the density ratio of CD9 to actin in three experiments from frozen–thawed (0.087 ± 0.03) and fresh oocytes (0.28 ± 0.04). *P<.05. Statistical analysis was performed by using unpaired t tests. Wen. CD9 expression in frozen-thawed mouse oocytes. Fertil Steril 2007. Fertility and Sterility 2007 88, 526-529DOI: (10.1016/j.fertnstert.2006.11.130) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions