Survival and growth of human preantral follicles after cryopreservation of ovarian tissue, follicle isolation and short-term xenografting  Fernanda Paulini,

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Survival and growth of human preantral follicles after cryopreservation of ovarian tissue, follicle isolation and short-term xenografting  Fernanda Paulini, Janice M.V. Vilela, Maria Costanza Chiti, Jacques Donnez, Pascale Jadoul, Marie-Madeleine Dolmans, Christiani A. Amorim  Reproductive BioMedicine Online  Volume 33, Issue 3, Pages 425-432 (September 2016) DOI: 10.1016/j.rbmo.2016.05.003 Copyright © 2016 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Micrograph of a haematoxylin-eosin stained section of a day 0 clot showing random groups of cells and follicular structures in a fibrin clot. Reproductive BioMedicine Online 2016 33, 425-432DOI: (10.1016/j.rbmo.2016.05.003) Copyright © 2016 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 (A) Viability testing of human ovarian follicles soon after isolation. Light microscopy pictures of isolated follicles at 20× magnification; (B and C) follicle viability assessment by calcein-AM and ethidium homodimer-I. Reproductive BioMedicine Online 2016 33, 425-432DOI: (10.1016/j.rbmo.2016.05.003) Copyright © 2016 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Primary follicle and ovarian cells in a fibrin clot on day 7 stained with haematoxylin-eosin. Reproductive BioMedicine Online 2016 33, 425-432DOI: (10.1016/j.rbmo.2016.05.003) Copyright © 2016 Reproductive Healthcare Ltd. Terms and Conditions

Figure 4 Proportions of primordial, primary and secondary follicles on days 0 and 7 (error bars represent 95% confidence intervals). Reproductive BioMedicine Online 2016 33, 425-432DOI: (10.1016/j.rbmo.2016.05.003) Copyright © 2016 Reproductive Healthcare Ltd. Terms and Conditions

Figure 5 mmunohistochemical analyses. (A) Small secondary follicle with Ki67-positive granulosa cells in brown (fibrin group, day 7); (B) TUNEL assay showing 100% of granulosa cells to be viable after 7 days of grafting (fibrin group); (C) inhibin-alpha activity identified by brown staining in a preantral follicle (fibrin-hyaluronic acid group, day 7). Reproductive BioMedicine Online 2016 33, 425-432DOI: (10.1016/j.rbmo.2016.05.003) Copyright © 2016 Reproductive Healthcare Ltd. Terms and Conditions