648. DMSO Shock Yields Highly Efficient Transient Transfection by PEI/DNA Nanoparticles  Molecular Therapy  Volume 20, (May 2012) DOI: 10.1016/S1525-0016(16)36452-8 Copyright © 2012 The American Society of Gene & Cell Therapy Terms and Conditions

Figure 1 Effect of transfection conditions on transfection efficiency (percent GFP positive cells) and average GFP expression level in human Schwann cells. Bars = average + standard deviation. Average GFP expression level was significantly different between all groups (p < 0.01, n=12, REGW test). Cell metabolic activity was between 78-95% relative to the control group for all conditions, as measured by WST-1 assay. (1) Krick K et al, Curr Opin Biotechnol. 2011, 22, 741. (2) Wang AJ et al, Biomaterials 2011, 32, 5023. (3) Kunath K. et al, J. Control. Release 2003, 89, 113. (4) Kawai S and Nishizawa M., Mol. Cell. Biol. 1984, 4, 1172. (5) Lewis WH, et al, Somatic Cell Genet 1980, 6, 333. Molecular Therapy 2012 20, DOI: (10.1016/S1525-0016(16)36452-8) Copyright © 2012 The American Society of Gene & Cell Therapy Terms and Conditions