Suppression of lymphocyte proliferation in vitro by bovine caseins hydrolyzed with Lactobacillus casei GG–derived enzymes Yelda Sütas, MD,a, Esa Soppi,

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Presentation transcript:

Suppression of lymphocyte proliferation in vitro by bovine caseins hydrolyzed with Lactobacillus casei GG–derived enzymes Yelda Sütas, MD,a, Esa Soppi, MD,a, Hannu Korhonen, PhD,b, Eeva-Liisa Syväoja, PhD,c, Maija Saxelin, PhD,c, Timo Rokka, MSc,d, Erika Isolauri, MDa Journal of Allergy and Clinical Immunology Volume 98, Issue 1, Pages 216-224 (July 1996) DOI: 10.1016/S0091-6749(96)70245-2 Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 1 Peptide profile (A-D) of L. casei GG–fermented milk hydrolyzed with pepsin and trypsin. Fractions B to C and subfractions BI to CIV were isolated by fast protein liquid chromatography with a Pep RP HR 16/10 column (Pharmacia) and 0.1% TFA in acetonitrile at a flow rate of 7 ml/min. Journal of Allergy and Clinical Immunology 1996 98, 216-224DOI: (10.1016/S0091-6749(96)70245-2) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 2 Mitogen-induced proliferative responses of PBMCs in vitro to milk hydrolyzed with pepsin and trypsin. The results are expressed as mean counts per minute for cultures without hydrolyzed milk (PHA + RPMI 1640) and with hydrolyzed milk (PHA + hydrolyzed milk) at three concentrations (0.1, 10, and 1000 μg/ml). Horizontal lines represent geometric means of counts per minute of the eight experiments, enumerated the same for each individual. Journal of Allergy and Clinical Immunology 1996 98, 216-224DOI: (10.1016/S0091-6749(96)70245-2) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 3 Mitogen-induced proliferative responses of PBMCs in vitro to casein (A) and casein components, β- (B), α s1 (C), and κ-casein (D) hydrolyzed with pepsin and trypsin. Results are expressed as mean counts per minute for cultures without hydrolyzed product (PHA + RPMI 1640) and with hydrolyzed product at three concentrations (0.1, 10, and 1000 μg/ml). Horizontal lines represent the geometric mean of counts per minute of the six experiments, enumerated the same for each individual. Journal of Allergy and Clinical Immunology 1996 98, 216-224DOI: (10.1016/S0091-6749(96)70245-2) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 3 Mitogen-induced proliferative responses of PBMCs in vitro to casein (A) and casein components, β- (B), α s1 (C), and κ-casein (D) hydrolyzed with pepsin and trypsin. Results are expressed as mean counts per minute for cultures without hydrolyzed product (PHA + RPMI 1640) and with hydrolyzed product at three concentrations (0.1, 10, and 1000 μg/ml). Horizontal lines represent the geometric mean of counts per minute of the six experiments, enumerated the same for each individual. Journal of Allergy and Clinical Immunology 1996 98, 216-224DOI: (10.1016/S0091-6749(96)70245-2) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 3 Mitogen-induced proliferative responses of PBMCs in vitro to casein (A) and casein components, β- (B), α s1 (C), and κ-casein (D) hydrolyzed with pepsin and trypsin. Results are expressed as mean counts per minute for cultures without hydrolyzed product (PHA + RPMI 1640) and with hydrolyzed product at three concentrations (0.1, 10, and 1000 μg/ml). Horizontal lines represent the geometric mean of counts per minute of the six experiments, enumerated the same for each individual. Journal of Allergy and Clinical Immunology 1996 98, 216-224DOI: (10.1016/S0091-6749(96)70245-2) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 3 Mitogen-induced proliferative responses of PBMCs in vitro to casein (A) and casein components, β- (B), α s1 (C), and κ-casein (D) hydrolyzed with pepsin and trypsin. Results are expressed as mean counts per minute for cultures without hydrolyzed product (PHA + RPMI 1640) and with hydrolyzed product at three concentrations (0.1, 10, and 1000 μg/ml). Horizontal lines represent the geometric mean of counts per minute of the six experiments, enumerated the same for each individual. Journal of Allergy and Clinical Immunology 1996 98, 216-224DOI: (10.1016/S0091-6749(96)70245-2) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 4 Mitogen-induced proliferative responses of PBMCs in vitro to casein (A) and casein components, β (B), α s1 (C), and κ-casein (D) additionally hydrolyzed with L. casei GG–derived enzymes. Results are expressed as the mean counts per minute for cultures without hydrolyzed product (PHA + RPMI 1640) and with hydrolyzed product at three concentrations (0.1, 10, and 1000 μg/ml). Horizontal lines represent the geometric mean of counts per minute of the six experiments, enumerated the same for each individual. Journal of Allergy and Clinical Immunology 1996 98, 216-224DOI: (10.1016/S0091-6749(96)70245-2) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 4 Mitogen-induced proliferative responses of PBMCs in vitro to casein (A) and casein components, β (B), α s1 (C), and κ-casein (D) additionally hydrolyzed with L. casei GG–derived enzymes. Results are expressed as the mean counts per minute for cultures without hydrolyzed product (PHA + RPMI 1640) and with hydrolyzed product at three concentrations (0.1, 10, and 1000 μg/ml). Horizontal lines represent the geometric mean of counts per minute of the six experiments, enumerated the same for each individual. Journal of Allergy and Clinical Immunology 1996 98, 216-224DOI: (10.1016/S0091-6749(96)70245-2) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 4 Mitogen-induced proliferative responses of PBMCs in vitro to casein (A) and casein components, β (B), α s1 (C), and κ-casein (D) additionally hydrolyzed with L. casei GG–derived enzymes. Results are expressed as the mean counts per minute for cultures without hydrolyzed product (PHA + RPMI 1640) and with hydrolyzed product at three concentrations (0.1, 10, and 1000 μg/ml). Horizontal lines represent the geometric mean of counts per minute of the six experiments, enumerated the same for each individual. Journal of Allergy and Clinical Immunology 1996 98, 216-224DOI: (10.1016/S0091-6749(96)70245-2) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 4 Mitogen-induced proliferative responses of PBMCs in vitro to casein (A) and casein components, β (B), α s1 (C), and κ-casein (D) additionally hydrolyzed with L. casei GG–derived enzymes. Results are expressed as the mean counts per minute for cultures without hydrolyzed product (PHA + RPMI 1640) and with hydrolyzed product at three concentrations (0.1, 10, and 1000 μg/ml). Horizontal lines represent the geometric mean of counts per minute of the six experiments, enumerated the same for each individual. Journal of Allergy and Clinical Immunology 1996 98, 216-224DOI: (10.1016/S0091-6749(96)70245-2) Copyright © 1996 Mosby, Inc. Terms and Conditions