An outbreak of skin and soft tissue infection caused by Mycobacterium abscessus following acupuncture S.-J. Koh, T. Song, Y.A. Kang, J.W. Choi, K.J.

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An outbreak of skin and soft tissue infection caused by Mycobacterium abscessus following acupuncture S.-J. Koh, T. Song, Y.A. Kang, J.W. Choi, K.J. Chang, C.S. Chu, J.G. Jeong, J.-Y. Lee, M.-K. Song, H.-Y. Sung, Y.H. Kang, J.-J. Yim Clinical Microbiology and Infection Volume 16, Issue 7, Pages 895-901 (July 2010) DOI: 10.1111/j.1469-0691.2009.03026.x Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Typical skin lesions due to Mycobacterium abscessus infection after acupuncture. Clinical Microbiology and Infection 2010 16, 895-901DOI: (10.1111/j.1469-0691.2009.03026.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Comparative analysis of representative Mycobacterium abscessus isolates by randomly amplified polymorphic DNA PCR (RAPD-PCR) and pulsed-field gel electrophoresis (PFGE). (a) DNA fragments amplified by RAPD-PCR using the primer IS986-FP [18]. (b) DNA fragments amplified by RAPD-PCR using the primer INS-2 [18]. (c) PFGE patterns of genomic DNA digested with AseI. Lanes 1 and 14: size markers. Lanes 2–5: environmental isolates from the diluted glutaraldehyde solution (lane 2), the electrode of an interferential current therapy device (lane 3), the bed (lane 4) and the sink (lane 5) in the treatment room. Lanes 6–10: clinical isolates from patients who developed M. abscessus infection at different time-points during the period of the outbreak. Lanes 11–13: M. abscessus isolates from pulmonary infections unrelated to the outbreak. All of the isolates from patient and environmental samples generated identical patterns, implying genetic relatedness of the isolates. Clinical Microbiology and Infection 2010 16, 895-901DOI: (10.1111/j.1469-0691.2009.03026.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions